Restriction map for
pF1KB5451
(Linear) (Six-base) MAPSORT of: KIBB5451.seq Check: 844 from: 1 to: 1116
KIBB5451.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 46 1116
Size: 46 1070
AatII G_ACGT'C
Cuts at: 0 469 1116
Size: 469 647
AccI GT'mk_AC
Cuts at: 0 140 1116
Size: 140 976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 545 1057 1116
Size: 115 430 512 59
Fragments arranged by size:
512 430 115 59
AflIII A'CryG_T
Cuts at: 0 1043 1116
Size: 1043 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1102 1116
Size: 1102 14
AlwNI CAG_nnn'CTG
Cuts at: 0 588 956 1116
Size: 588 368 160
Fragments arranged by size:
588 368 160
ApoI r'AATT_y
Cuts at: 0 176 323 513 811 1116
Size: 176 147 190 298 305
Fragments arranged by size:
305 298 190 176 147
AvaI C'yCGr_G
Cuts at: 0 231 1020 1116
Size: 231 789 96
Fragments arranged by size:
789 231 96
BanI G'GyrC_C
Cuts at: 0 130 1116
Size: 130 986
BanII G_rGCy'C
Cuts at: 0 272 847 1116
Size: 272 575 269
Fragments arranged by size:
575 272 269
BbeI G_GCGC'C
Cuts at: 0 134 1116
Size: 134 982
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 1116
Size: 551 565
BglI GCCn_nnn'nGGC
Cuts at: 0 620 1116
Size: 620 496
BmgBI CAC'GTC
Cuts at: 0 419 1116
Size: 419 697
BsaI GGTCTCn'nnnn_
Cuts at: 0 868 1116
Size: 868 248
BsaAI yAC'GTr
Cuts at: 0 1086 1116
Size: 1086 30
BsaHI Gr'CG_yC
Cuts at: 0 131 311 466 1109 1116
Size: 131 180 155 643 7
Fragments arranged by size:
643 180 155 131 7
BsaWI w'CCGG_w
Cuts at: 0 116 1116
Size: 116 1000
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 1116
Size: 280 836
BseYI C'CCAG_C
Cuts at: 0 706 943 1116
Size: 706 237 173
Fragments arranged by size:
706 237 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1116
Size: 1108 8
BsiHKAI G_wGCw'C
Cuts at: 0 272 1116
Size: 272 844
Bsp1286I G_dGCh'C
Cuts at: 0 272 847 1116
Size: 272 575 269
Fragments arranged by size:
575 272 269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 46 1116
Size: 46 1070
BsrDI GCAATG_nn'
Cuts at: 0 201 1116
Size: 201 915
BsrFI r'CCGG_y
Cuts at: 0 190 1116
Size: 190 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 956 1116
Size: 956 160
BstEII G'GTnAC_C
Cuts at: 0 984 1116
Size: 984 132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 796 1116
Size: 633 163 320
Fragments arranged by size:
633 320 163
BtgI C'CryG_G
Cuts at: 0 460 1116
Size: 460 656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 282 1116
Size: 282 834
EaeI y'GGCC_r
Cuts at: 0 188 341 1097 1116
Size: 188 153 756 19
Fragments arranged by size:
756 188 153 19
EarI CTCTTCn'nnn_
Cuts at: 0 866 1063 1116
Size: 866 197 53
Fragments arranged by size:
866 197 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 916 1116
Size: 916 200
EcoICRI GAG'CTC
Cuts at: 0 270 1116
Size: 270 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 545 1057 1116
Size: 115 430 512 59
Fragments arranged by size:
512 430 115 59
EcoO109I rG'GnC_Cy
Cuts at: 0 757 1116
Size: 757 359
EcoRI G'AATT_C
Cuts at: 0 323 1116
Size: 323 793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 883 1116
Size: 609 274 233
Fragments arranged by size:
609 274 233
HaeII r_GCGC'y
Cuts at: 0 134 1116
Size: 134 982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 281 313 1116
Size: 75 32 174 32 803
Fragments arranged by size:
803 174 75 32 32
KasI G'GCGC_C
Cuts at: 0 130 1116
Size: 130 986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 872 1116
Size: 350 522 244
Fragments arranged by size:
522 350 244
MscI TGG'CCA
Cuts at: 0 343 1099 1116
Size: 343 756 17
Fragments arranged by size:
756 343 17
MslI CAynn'nnrTG
Cuts at: 0 631 1013 1084 1116
Size: 631 382 71 32
Fragments arranged by size:
631 382 71 32
MspA1I CmG'CkG
Cuts at: 0 172 279 898 947 1116
Size: 172 107 619 49 169
Fragments arranged by size:
619 172 169 107 49
NaeI GCC'GGC
Cuts at: 0 192 1116
Size: 192 924
NarI GG'CG_CC
Cuts at: 0 131 1116
Size: 131 985
NgoMIV G'CCGG_C
Cuts at: 0 190 1116
Size: 190 926
PflMI CCAn_nnn'nTGG
Cuts at: 0 541 1116
Size: 541 575
PspXI vC'TCGA_Gb
Cuts at: 0 1020 1116
Size: 1020 96
PvuII CAG'CTG
Cuts at: 0 279 1116
Size: 279 837
SacI G_AGCT'C
Cuts at: 0 272 1116
Size: 272 844
SapI GCTCTTCn'nnn_
Cuts at: 0 1063 1116
Size: 1063 53
SfoI GGC'GCC
Cuts at: 0 132 1116
Size: 132 984
SmlI C'TyrA_G
Cuts at: 0 1020 1116
Size: 1020 96
StyI C'CwwG_G
Cuts at: 0 446 695 753 1116
Size: 446 249 58 363
Fragments arranged by size:
446 363 249 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 665 1116
Size: 665 451
TatI w'GTAC_w
Cuts at: 0 1046 1116
Size: 1046 70
XbaI T'CTAG_A
Cuts at: 0 180 1116
Size: 180 936
XhoI C'TCGA_G
Cuts at: 0 1020 1116
Size: 1020 96
ZraI GAC'GTC
Cuts at: 0 467 1116
Size: 467 649
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BglI BmgBI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstEII BstXI BtgI BtgZI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PflMI PspXI PvuII SacI SapI SfoI
SmlI StyI TaqII TatI XbaI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XcmI XmaI XmnI