(Linear) (Six-base) MAPSORT of: KIBB5448.seq  Check: 2665  from: 1  to: 579



KIBB5448.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     443     579 
   Size:         57     386     136

  Fragments arranged by size: 

                 386     136      57
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     218     251     579 
   Size:        218      33     328

  Fragments arranged by size: 

                 328     218      33
 
BanI G'GyrC_C

Cuts at:      0     566     579 
   Size:        566      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      63     579 
   Size:         63     516
 
Bme1580I G_kGCm'C

Cuts at:      0     107     332     579 
   Size:        107     225     247

  Fragments arranged by size: 

                 247     225     107
 
BmgBI CAC'GTC

Cuts at:      0     547     579 
   Size:        547      32
 
BmtI G_CTAG'C

Cuts at:      0     394     579 
   Size:        394     185
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     548     579 
   Size:        548      31
 
BsaBI GATnn'nnATC

Cuts at:      0     197     579 
   Size:        197     382
 
BsaWI w'CCGG_w

Cuts at:      0      11     579 
   Size:         11     568
 
BseYI C'CCAG_C

Cuts at:      0     181     579 
   Size:        181     398
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     579 
   Size:        377     202
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     332     377     579 
   Size:        107     225      45     202

  Fragments arranged by size: 

                 225     202     107      45
 
BspEI T'CCGG_A

Cuts at:      0      11     579 
   Size:         11     568
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     144     579 
   Size:        144     435
 
BsrFI r'CCGG_y

Cuts at:      0     411     579 
   Size:        411     168
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     293     339     579 
   Size:        293      46     240

  Fragments arranged by size: 

                 293     240      46
 
BstYI r'GATC_y

Cuts at:      0     357     579 
   Size:        357     222
 
Bsu36I CC'TnA_GG

Cuts at:      0     205     579 
   Size:        205     374
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      96     131     579 
   Size:         96      35     448

  Fragments arranged by size: 

                 448      96      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     443     579 
   Size:         57     386     136

  Fragments arranged by size: 

                 386     136      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     425     579 
   Size:        425     154
 
EcoO109I rG'GnC_Cy

Cuts at:      0     209     579 
   Size:        209     370
 
EcoRV GAT'ATC

Cuts at:      0     135     579 
   Size:        135     444
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      75     579 
   Size:         75     504
 
HaeII r_GCGC'y

Cuts at:      0     458     579 
   Size:        458     121
 
MspA1I CmG'CkG

Cuts at:      0     181     579 
   Size:        181     398
 
NheI G'CTAG_C

Cuts at:      0     390     579 
   Size:        390     189
 
NspI r_CATG'y

Cuts at:      0      59     579 
   Size:         59     520
 
PvuII CAG'CTG

Cuts at:      0     181     579 
   Size:        181     398
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     554     579 
   Size:        554      25
 

 Enzymes that do cut:

     AcuI     BaeI     BanI     BbsI Bme1580I    BmgBI     BmtI     BplI
    BsaBI    BsaWI    BseYI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI
    BstXI    BstYI   Bsu36I    CspCI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI    HaeII   MspA1I     NheI     NspI    PvuII    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI