Restriction map for
pF1KB5446
(Linear) (Six-base) MAPSORT of: KIBB5446.seq Check: 9783 from: 1 to: 1965
KIBB5446.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1048 1965
Size: 1048 917
AleI CACnn'nnGTG
Cuts at: 0 1636 1965
Size: 1636 329
AlwNI CAG_nnn'CTG
Cuts at: 0 42 707 1689 1701 1965
Size: 42 665 982 12 264
Fragments arranged by size:
982 665 264 42 12
ApaI G_GGCC'C
Cuts at: 0 540 884 1965
Size: 540 344 1081
Fragments arranged by size:
1081 540 344
ApoI r'AATT_y
Cuts at: 0 1115 1965
Size: 1115 850
AvaI C'yCGr_G
Cuts at: 0 49 1746 1965
Size: 49 1697 219
Fragments arranged by size:
1697 219 49
BamHI G'GATC_C
Cuts at: 0 1838 1965
Size: 1838 127
BanI G'GyrC_C
Cuts at: 0 127 948 1048 1965
Size: 127 821 100 917
Fragments arranged by size:
917 821 127 100
BanII G_rGCy'C
Cuts at: 0 540 656 884 1451 1617 1889 1965
Size: 540 116 228 567 166 272 76
Fragments arranged by size:
567 540 272 228 166 116 76
BbvCI CC'TCA_GC
Cuts at: 0 43 1525 1965
Size: 43 1482 440
Fragments arranged by size:
1482 440 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 1708 1742 1965
Size: 26 1682 34 223
Fragments arranged by size:
1682 223 34 26
BfrBI ATG'CAT
Cuts at: 0 507 1298 1965
Size: 507 791 667
Fragments arranged by size:
791 667 507
BglII A'GATC_T
Cuts at: 0 1359 1965
Size: 1359 606
BlpI GC'TnA_GC
Cuts at: 0 134 1965
Size: 134 1831
Bme1580I G_kGCm'C
Cuts at: 0 440 540 701 884 1965
Size: 440 100 161 183 1081
Fragments arranged by size:
1081 440 183 161 100
BmrI ACTGGGnnnn_n'
Cuts at: 0 434 468 1095 1965
Size: 434 34 627 870
Fragments arranged by size:
870 627 434 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1358 1639 1965
Size: 394 964 281 326
Fragments arranged by size:
964 394 326 281
Bpu10I CC'TnA_GC
Cuts at: 0 43 1070 1525 1965
Size: 43 1027 455 440
Fragments arranged by size:
1027 455 440 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 642 1740 1965
Size: 391 251 1098 225
Fragments arranged by size:
1098 391 251 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 1965
Size: 382 1583
BsaBI GATnn'nnATC
Cuts at: 0 901 1965
Size: 901 1064
BsaWI w'CCGG_w
Cuts at: 0 1373 1965
Size: 1373 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 932 962 1965
Size: 310 30 592 30 1003
Fragments arranged by size:
1003 592 310 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 1965
Size: 400 1565
BseYI C'CCAG_C
Cuts at: 0 414 447 700 966 1542 1787 1965
Size: 414 33 253 266 576 245 178
Fragments arranged by size:
576 414 266 253 245 178 33
BsiHKAI G_wGCw'C
Cuts at: 0 281 1355 1451 1470 1889 1965
Size: 281 1074 96 19 419 76
Fragments arranged by size:
1074 419 281 96 76 19
BsmI GAATG_Cn'
Cuts at: 0 1300 1494 1965
Size: 1300 194 471
Fragments arranged by size:
1300 471 194
Bsp1286I G_dGCh'C
Cuts at: 0 281 440 540 656 701 884 1355 1451
Size: 281 159 100 116 45 183 471 96
Cuts at: 1451 1470 1617 1889 1965
Size: 19 147 272 76
Fragments arranged by size:
471 281 272 183 159 147 116 100
96 76 45 19
BspEI T'CCGG_A
Cuts at: 0 1373 1965
Size: 1373 592
BspHI T'CATG_A
Cuts at: 0 1325 1965
Size: 1325 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1036 1965
Size: 1036 929
BsrBI CCG'CTC
Cuts at: 0 133 1929 1965
Size: 133 1796 36
Fragments arranged by size:
1796 133 36
BsrDI GCAATG_nn'
Cuts at: 0 1064 1965
Size: 1064 901
BsrFI r'CCGG_y
Cuts at: 0 192 1965
Size: 192 1773
BstEII G'GTnAC_C
Cuts at: 0 1941 1965
Size: 1941 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 1638 1737 1920 1965
Size: 447 1191 99 183 45
Fragments arranged by size:
1191 447 183 99 45
BstYI r'GATC_y
Cuts at: 0 804 1359 1376 1759 1838 1965
Size: 804 555 17 383 79 127
Fragments arranged by size:
804 555 383 127 79 17
BtgI C'CryG_G
Cuts at: 0 876 1184 1223 1730 1781 1965
Size: 876 308 39 507 51 184
Fragments arranged by size:
876 507 308 184 51 39
BtsI GCAGTG_nn'
Cuts at: 0 1470 1965
Size: 1470 495
DraIII CAC_nnn'GTG
Cuts at: 0 1817 1965
Size: 1817 148
EaeI y'GGCC_r
Cuts at: 0 17 194 632 1853 1965
Size: 17 177 438 1221 112
Fragments arranged by size:
1221 438 177 112 17
EarI CTCTTCn'nnn_
Cuts at: 0 281 1965
Size: 281 1684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 71 217 1965
Size: 71 146 1748
Fragments arranged by size:
1748 146 71
EcoICRI GAG'CTC
Cuts at: 0 1449 1887 1965
Size: 1449 438 78
Fragments arranged by size:
1449 438 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1358 1639 1965
Size: 394 964 281 326
Fragments arranged by size:
964 394 326 281
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1768 1965
Size: 1768 197
EcoO109I rG'GnC_Cy
Cuts at: 0 402 419 536 759 881 1751 1965
Size: 402 17 117 223 122 870 214
Fragments arranged by size:
870 402 223 214 122 117 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1497 1965
Size: 1497 468
HaeII r_GCGC'y
Cuts at: 0 79 1965
Size: 79 1886
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 661 693 1965
Size: 310 32 319 32 1272
Fragments arranged by size:
1272 319 310 32 32
HindIII A'AGCT_T
Cuts at: 0 739 1965
Size: 739 1226
KpnI G_GTAC'C
Cuts at: 0 1052 1965
Size: 1052 913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1570 1965
Size: 1570 395
MslI CAynn'nnrTG
Cuts at: 0 1636 1735 1965
Size: 1636 99 230
Fragments arranged by size:
1636 230 99
MspA1I CmG'CkG
Cuts at: 0 138 1524 1550 1565 1698 1965
Size: 138 1386 26 15 133 267
Fragments arranged by size:
1386 267 138 133 26 15
NcoI C'CATG_G
Cuts at: 0 876 1184 1730 1965
Size: 876 308 546 235
Fragments arranged by size:
876 546 308 235
NsiI A_TGCA'T
Cuts at: 0 509 1300 1965
Size: 509 791 665
Fragments arranged by size:
791 665 509
NspI r_CATG'y
Cuts at: 0 507 1965
Size: 507 1458
PasI CC'CwG_GG
Cuts at: 0 531 1965
Size: 531 1434
PflMI CCAn_nnn'nTGG
Cuts at: 0 1308 1965
Size: 1308 657
PfoI T'CCnGG_A
Cuts at: 0 1136 1965
Size: 1136 829
PspOMI G'GGCC_C
Cuts at: 0 536 880 1965
Size: 536 344 1085
Fragments arranged by size:
1085 536 344
PstI C_TGCA'G
Cuts at: 0 1169 1424 1697 1833 1865 1965
Size: 1169 255 273 136 32 100
Fragments arranged by size:
1169 273 255 136 100 32
PvuII CAG'CTG
Cuts at: 0 138 1524 1965
Size: 138 1386 441
Fragments arranged by size:
1386 441 138
SacI G_AGCT'C
Cuts at: 0 1451 1889 1965
Size: 1451 438 76
Fragments arranged by size:
1451 438 76
SapI GCTCTTCn'nnn_
Cuts at: 0 281 1965
Size: 281 1684
SexAI A'CCwGG_T
Cuts at: 0 1813 1965
Size: 1813 152
SfcI C'TryA_G
Cuts at: 0 1165 1420 1693 1829 1861 1965
Size: 1165 255 273 136 32 104
Fragments arranged by size:
1165 273 255 136 104 32
SmlI C'TyrA_G
Cuts at: 0 406 657 1746 1755 1965
Size: 406 251 1089 9 210
Fragments arranged by size:
1089 406 251 210 9
SphI G_CATG'C
Cuts at: 0 507 1965
Size: 507 1458
StuI AGG'CCT
Cuts at: 0 1918 1965
Size: 1918 47
StyI C'CwwG_G
Cuts at: 0 197 513 876 1184 1730 1913 1919 1965
Size: 197 316 363 308 546 183 6 46
Fragments arranged by size:
546 363 316 308 197 183 46 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 439 487 958 1965
Size: 439 48 471 1007
Fragments arranged by size:
1007 471 439 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 932 964 1965
Size: 932 32 1001
Fragments arranged by size:
1001 932 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1191 1920 1965
Size: 1191 729 45
Fragments arranged by size:
1191 729 45
XhoI C'TCGA_G
Cuts at: 0 1746 1965
Size: 1746 219
XmnI GAAnn'nnTTC
Cuts at: 0 1685 1965
Size: 1685 280
Enzymes that do cut:
Acc65I AleI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbvCI BcgI BfrBI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstEII BstXI BstYI BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HindIII KpnI MmeI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PspOMI PstI PvuII
SacI SapI SexAI SfcI SmlI SphI StuI StyI
TaqII TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BciVI BclI BglI BmgBI
BmtI BplI BsaAI BsaHI BsgI BsiEI BsiWI BsmBI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI
FspI FspAI HincII HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XmaI ZraI