Restriction map for
pF1KB5444
(Linear) (Six-base) MAPSORT of: KIBB5444.seq Check: 5731 from: 1 to: 2172
KIBB5444.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1467 2172
Size: 1467 705
AccI GT'mk_AC
Cuts at: 0 260 2172
Size: 260 1912
Acc65I G'GTAC_C
Cuts at: 0 532 2172
Size: 532 1640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 2172
Size: 334 1838
AlwNI CAG_nnn'CTG
Cuts at: 0 2113 2172
Size: 2113 59
ApaLI G'TGCA_C
Cuts at: 0 13 2172
Size: 13 2159
ApoI r'AATT_y
Cuts at: 0 907 982 1282 1579 2172
Size: 907 75 300 297 593
Fragments arranged by size:
907 593 300 297 75
AvaI C'yCGr_G
Cuts at: 0 1172 2131 2172
Size: 1172 959 41
Fragments arranged by size:
1172 959 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 524 557 1099 1132 2172
Size: 524 33 542 33 1040
Fragments arranged by size:
1040 542 524 33 33
BamHI G'GATC_C
Cuts at: 0 2013 2172
Size: 2013 159
BanI G'GyrC_C
Cuts at: 0 532 2172
Size: 532 1640
BanII G_rGCy'C
Cuts at: 0 521 1017 1244 1547 2172
Size: 521 496 227 303 625
Fragments arranged by size:
625 521 496 303 227
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 944 1268 1334 1748 2172
Size: 467 477 324 66 414 424
Fragments arranged by size:
477 467 424 414 324 66
BbvCI CC'TCA_GC
Cuts at: 0 1925 2172
Size: 1925 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2128 2162 2172
Size: 2128 34 10
Fragments arranged by size:
2128 34 10
BclI T'GATC_A
Cuts at: 0 434 831 2048 2172
Size: 434 397 1217 124
Fragments arranged by size:
1217 434 397 124
BglI GCCn_nnn'nGGC
Cuts at: 0 52 2172
Size: 52 2120
Bme1580I G_kGCm'C
Cuts at: 0 17 2172
Size: 17 2155
BmrI ACTGGGnnnn_n'
Cuts at: 0 942 1374 2172
Size: 942 432 798
Fragments arranged by size:
942 798 432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 1198 1263 1314 2172
Size: 147 1051 65 51 858
Fragments arranged by size:
1051 858 147 65 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1168 1406 1642 1897 2172
Size: 503 665 238 236 255 275
Fragments arranged by size:
665 503 275 255 238 236
Bpu10I CC'TnA_GC
Cuts at: 0 1925 2172
Size: 1925 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1294 1558 2029 2172
Size: 1258 36 264 471 143
Fragments arranged by size:
1258 471 264 143 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 1605 2172
Size: 1605 567
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1315 1345 2172
Size: 1315 30 827
Fragments arranged by size:
1315 827 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 43 784 910 1654 1657 2172
Size: 40 3 741 126 744 3 515
Fragments arranged by size:
744 741 515 126 40 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1585 2172
Size: 380 1205 587
Fragments arranged by size:
1205 587 380
BsiHKAI G_wGCw'C
Cuts at: 0 17 1244 2172
Size: 17 1227 928
Fragments arranged by size:
1227 928 17
BsmI GAATG_Cn'
Cuts at: 0 334 612 1294 2172
Size: 334 278 682 878
Fragments arranged by size:
878 682 334 278
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1900 2156 2172
Size: 1900 256 16
Fragments arranged by size:
1900 256 16
Bsp1286I G_dGCh'C
Cuts at: 0 17 521 1017 1244 1547 2172
Size: 17 504 496 227 303 625
Fragments arranged by size:
625 504 496 303 227 17
BspHI T'CATG_A
Cuts at: 0 1697 1814 2172
Size: 1697 117 358
Fragments arranged by size:
1697 358 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1467 2172
Size: 1467 705
BsrBI CCG'CTC
Cuts at: 0 1809 2172
Size: 1809 363
BsrDI GCAATG_nn'
Cuts at: 0 311 991 2172
Size: 311 680 1181
Fragments arranged by size:
1181 680 311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 61 2172
Size: 61 2111
BstBI TT'CG_AA
Cuts at: 0 187 2172
Size: 187 1985
BstYI r'GATC_y
Cuts at: 0 1152 2013 2120 2172
Size: 1152 861 107 52
Fragments arranged by size:
1152 861 107 52
Bsu36I CC'TnA_GG
Cuts at: 0 5 236 1631 2172
Size: 5 231 1395 541
Fragments arranged by size:
1395 541 231 5
BtgI C'CryG_G
Cuts at: 0 18 510 1131 1847 2172
Size: 18 492 621 716 325
Fragments arranged by size:
716 621 492 325 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2153 2172
Size: 2153 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 1404 1440 2172
Size: 1404 36 732
Fragments arranged by size:
1404 732 36
EaeI y'GGCC_r
Cuts at: 0 1481 1862 2172
Size: 1481 381 310
Fragments arranged by size:
1481 381 310
EarI CTCTTCn'nnn_
Cuts at: 0 353 575 689 707 1249 1585 1655 2172
Size: 353 222 114 18 542 336 70 517
Fragments arranged by size:
542 517 353 336 222 114 70 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1060 2172
Size: 1060 1112
EcoICRI GAG'CTC
Cuts at: 0 1242 2172
Size: 1242 930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 503 1168 1406 1642 1897 2172
Size: 334 169 665 238 236 255 275
Fragments arranged by size:
665 334 275 255 238 236 169
EcoO109I rG'GnC_Cy
Cuts at: 0 1956 2172
Size: 1956 216
EcoRI G'AATT_C
Cuts at: 0 907 982 2172
Size: 907 75 1190
Fragments arranged by size:
1190 907 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 534 566 1384 1386 1416 1418 2112 2134
Size: 534 32 818 2 30 2 694 22
Cuts at: 2134 2144 2166 2172
Size: 10 22 6
Fragments arranged by size:
818 694 534 32 30 22 22 10
6 2 2
HincII GTy'rAC
Cuts at: 0 1143 2172
Size: 1143 1029
HindIII A'AGCT_T
Cuts at: 0 1312 2172
Size: 1312 860
KpnI G_GTAC'C
Cuts at: 0 536 2172
Size: 536 1636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 173 959 2054 2172
Size: 125 48 786 1095 118
Fragments arranged by size:
1095 786 125 118 48
MscI TGG'CCA
Cuts at: 0 1483 1864 2172
Size: 1483 381 308
Fragments arranged by size:
1483 381 308
MslI CAynn'nnrTG
Cuts at: 0 274 370 449 2172
Size: 274 96 79 1723
Fragments arranged by size:
1723 274 96 79
MspA1I CmG'CkG
Cuts at: 0 782 1094 2172
Size: 782 312 1078
Fragments arranged by size:
1078 782 312
NcoI C'CATG_G
Cuts at: 0 18 510 1847 2172
Size: 18 492 1337 325
Fragments arranged by size:
1337 492 325 18
PpuMI rG'GwC_Cy
Cuts at: 0 1956 2172
Size: 1956 216
PshAI GACnn'nnGTC
Cuts at: 0 1752 2172
Size: 1752 420
PstI C_TGCA'G
Cuts at: 0 2109 2172
Size: 2109 63
PvuII CAG'CTG
Cuts at: 0 1094 2172
Size: 1094 1078
SacI G_AGCT'C
Cuts at: 0 1244 2172
Size: 1244 928
SapI GCTCTTCn'nnn_
Cuts at: 0 353 1249 1585 2172
Size: 353 896 336 587
Fragments arranged by size:
896 587 353 336
ScaI AGT'ACT
Cuts at: 0 1558 2172
Size: 1558 614
SexAI A'CCwGG_T
Cuts at: 0 1958 2172
Size: 1958 214
SfcI C'TryA_G
Cuts at: 0 965 2105 2172
Size: 965 1140 67
Fragments arranged by size:
1140 965 67
SmlI C'TyrA_G
Cuts at: 0 1237 1309 1573 2008 2131 2172
Size: 1237 72 264 435 123 41
Fragments arranged by size:
1237 435 264 123 72 41
StyI C'CwwG_G
Cuts at: 0 18 149 510 1610 1847 2172
Size: 18 131 361 1100 237 325
Fragments arranged by size:
1100 361 325 237 131 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 1555 2172
Size: 904 651 617
Fragments arranged by size:
904 651 617
TatI w'GTAC_w
Cuts at: 0 1556 2172
Size: 1556 616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1315 1347 2172
Size: 1315 32 825
Fragments arranged by size:
1315 825 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 898 1617 1872 2172
Size: 898 719 255 300
Fragments arranged by size:
898 719 300 255
XhoI C'TCGA_G
Cuts at: 0 2131 2172
Size: 2131 41
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BclI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I HincII HindIII KpnI MmeI MscI MslI
MspA1I NcoI PpuMI PshAI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI StyI TaqII TatI TstI
XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BbeI
BciVI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BspEI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstZ17I BtsI ClaI CspCI
DraI DraIII EagI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI
XmnI ZraI