(Linear) (Six-base) MAPSORT of: KIBB5430.seq  Check: 2725  from: 1  to: 453



KIBB5430.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     453 
   Size:         86     367
 
ApaI G_GGCC'C

Cuts at:      0     336     453 
   Size:        336     117
 
AvaI C'yCGr_G

Cuts at:      0     363     453 
   Size:        363      90
 
BanI G'GyrC_C

Cuts at:      0       3     453 
   Size:          3     450
 
BanII G_rGCy'C

Cuts at:      0     336     354     453 
   Size:        336      18      99

  Fragments arranged by size: 

                 336      99      18
 
BglI GCCn_nnn'nGGC

Cuts at:      0     340     453 
   Size:        340     113
 
Bme1580I G_kGCm'C

Cuts at:      0     336     453 
   Size:        336     117
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     330     453 
   Size:        330     123
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      62      92     453 
   Size:         62      30     361

  Fragments arranged by size: 

                 361      62      30
 
BseYI C'CCAG_C

Cuts at:      0     261     453 
   Size:        261     192
 
Bsp1286I G_dGCh'C

Cuts at:      0     336     354     453 
   Size:        336      18      99

  Fragments arranged by size: 

                 336      99      18
 
BsrBI CCG'CTC

Cuts at:      0     362     453 
   Size:        362      91
 
Bsu36I CC'TnA_GG

Cuts at:      0      56     453 
   Size:         56     397
 
BtgI C'CryG_G

Cuts at:      0     438     453 
   Size:        438      15
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     121     453 
   Size:        121     332
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     396     453 
   Size:        396      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     453 
   Size:         86     367
 
EcoO109I rG'GnC_Cy

Cuts at:      0     332     453 
   Size:        332     121
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     453 
   Size:        190      32     231

  Fragments arranged by size: 

                 231     190      32
 
NdeI CA'TA_TG

Cuts at:      0     213     453 
   Size:        213     240
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240     453 
   Size:        240     213
 
PspOMI G'GGCC_C

Cuts at:      0     332     453 
   Size:        332     121
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     340     453 
   Size:        340     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     209     453 
   Size:        209     244
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      44      76     453 
   Size:         44      32     377

  Fragments arranged by size: 

                 377      44      32
 

 Enzymes that do cut:

     AcuI     ApaI     AvaI     BanI    BanII     BglI Bme1580I     BmrI
    BsaXI    BseYI Bsp1286I    BsrBI   Bsu36I     BtgI     DrdI     EciI
  Eco57MI EcoO109I    Hin4I     NdeI    PflMI   PspOMI     SfiI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI