Restriction map for
pF1KB5427
(Linear) (Six-base) MAPSORT of: KIBB5427.seq Check: 6877 from: 1 to: 1134
KIBB5427.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 558 1134
Size: 558 576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 812 1134
Size: 40 772 322
Fragments arranged by size:
772 322 40
AlwNI CAG_nnn'CTG
Cuts at: 0 33 975 1125 1134
Size: 33 942 150 9
Fragments arranged by size:
942 150 33 9
AvaI C'yCGr_G
Cuts at: 0 496 575 1134
Size: 496 79 559
Fragments arranged by size:
559 496 79
BamHI G'GATC_C
Cuts at: 0 770 1134
Size: 770 364
BanI G'GyrC_C
Cuts at: 0 874 897 1134
Size: 874 23 237
Fragments arranged by size:
874 237 23
BanII G_rGCy'C
Cuts at: 0 352 1093 1134
Size: 352 741 41
Fragments arranged by size:
741 352 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 417 788 800 992 1023 1134
Size: 39 378 371 12 192 31 111
Fragments arranged by size:
378 371 192 111 39 31 12
BclI T'GATC_A
Cuts at: 0 986 1134
Size: 986 148
BglI GCCn_nnn'nGGC
Cuts at: 0 906 1134
Size: 906 228
Bme1580I G_kGCm'C
Cuts at: 0 211 532 877 902 1134
Size: 211 321 345 25 232
Fragments arranged by size:
345 321 232 211 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 336 1134
Size: 214 122 798
Fragments arranged by size:
798 214 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 232 583 672 1134
Size: 156 76 351 89 462
Fragments arranged by size:
462 351 156 89 76
BsaHI Gr'CG_yC
Cuts at: 0 889 1134
Size: 889 245
BsaWI w'CCGG_w
Cuts at: 0 137 1134
Size: 137 997
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 1134
Size: 187 30 917
Fragments arranged by size:
917 187 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 100 660 871 1134
Size: 97 3 560 211 263
Fragments arranged by size:
560 263 211 97 3
BseYI C'CCAG_C
Cuts at: 0 720 1134
Size: 720 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1134
Size: 805 329
BsiHKAI G_wGCw'C
Cuts at: 0 811 956 1134
Size: 811 145 178
Fragments arranged by size:
811 178 145
BsmI GAATG_Cn'
Cuts at: 0 220 1134
Size: 220 914
BsmBI CGTCTCn'nnnn_
Cuts at: 0 880 1134
Size: 880 254
Bsp1286I G_dGCh'C
Cuts at: 0 211 352 532 811 877 902 956 1093
Size: 211 141 180 279 66 25 54 137
Cuts at: 1093 1134
Size: 41
Fragments arranged by size:
279 211 180 141 137 66 54 41
25
BspEI T'CCGG_A
Cuts at: 0 137 1134
Size: 137 997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 558 1134
Size: 558 576
BsrBI CCG'CTC
Cuts at: 0 995 1134
Size: 995 139
BsrGI T'GTAC_A
Cuts at: 0 682 1134
Size: 682 452
BssHII G'CGCG_C
Cuts at: 0 237 1134
Size: 237 897
BssSI C'ACGA_G
Cuts at: 0 922 1134
Size: 922 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 215 1134
Size: 215 919
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1134
Size: 360 774
BstYI r'GATC_y
Cuts at: 0 770 1134
Size: 770 364
Bsu36I CC'TnA_GG
Cuts at: 0 69 1134
Size: 69 1065
BtgI C'CryG_G
Cuts at: 0 893 1134
Size: 893 241
BtsI GCAGTG_nn'
Cuts at: 0 715 1134
Size: 715 419
EaeI y'GGCC_r
Cuts at: 0 362 1013 1134
Size: 362 651 121
Fragments arranged by size:
651 362 121
EarI CTCTTCn'nnn_
Cuts at: 0 103 139 1134
Size: 103 36 995
Fragments arranged by size:
995 103 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 214 336 812 1134
Size: 40 174 122 476 322
Fragments arranged by size:
476 322 174 122 40
EcoO109I rG'GnC_Cy
Cuts at: 0 357 1134
Size: 357 777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 864 1134
Size: 150 714 270
Fragments arranged by size:
714 270 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 793 825 1075 1107 1134
Size: 187 32 574 32 250 32 27
Fragments arranged by size:
574 250 187 32 32 32 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1134
Size: 736 398
MscI TGG'CCA
Cuts at: 0 364 1015 1134
Size: 364 651 119
Fragments arranged by size:
651 364 119
MslI CAynn'nnrTG
Cuts at: 0 418 568 640 1134
Size: 418 150 72 494
Fragments arranged by size:
494 418 150 72
MspA1I CmG'CkG
Cuts at: 0 180 670 1134
Size: 180 490 464
Fragments arranged by size:
490 464 180
NcoI C'CATG_G
Cuts at: 0 893 1134
Size: 893 241
NspI r_CATG'y
Cuts at: 0 567 1134
Size: 567 567
PflMI CCAn_nnn'nTGG
Cuts at: 0 975 1134
Size: 975 159
PfoI T'CCnGG_A
Cuts at: 0 485 1134
Size: 485 649
PpuMI rG'GwC_Cy
Cuts at: 0 357 1134
Size: 357 777
PstI C_TGCA'G
Cuts at: 0 154 1134
Size: 154 980
PvuII CAG'CTG
Cuts at: 0 180 670 1134
Size: 180 490 464
Fragments arranged by size:
490 464 180
SapI GCTCTTCn'nnn_
Cuts at: 0 103 139 1134
Size: 103 36 995
Fragments arranged by size:
995 103 36
ScaI AGT'ACT
Cuts at: 0 862 1134
Size: 862 272
SexAI A'CCwGG_T
Cuts at: 0 41 1134
Size: 41 1093
SfcI C'TryA_G
Cuts at: 0 150 864 1134
Size: 150 714 270
Fragments arranged by size:
714 270 150
SmaI CCC'GGG
Cuts at: 0 577 1134
Size: 577 557
SmlI C'TyrA_G
Cuts at: 0 171 247 598 651 1134
Size: 171 76 351 53 483
Fragments arranged by size:
483 351 171 76 53
SphI G_CATG'C
Cuts at: 0 567 1134
Size: 567 567
SrfI GCCC'GGGC
Cuts at: 0 577 1134
Size: 577 557
StyI C'CwwG_G
Cuts at: 0 353 581 623 893 1134
Size: 353 228 42 270 241
Fragments arranged by size:
353 270 241 228 42
TatI w'GTAC_w
Cuts at: 0 682 860 1134
Size: 682 178 274
Fragments arranged by size:
682 274 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 1134
Size: 1053 32 49
Fragments arranged by size:
1053 49 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 395 1134
Size: 372 23 739
Fragments arranged by size:
739 372 23
XmaI C'CCGG_G
Cuts at: 0 575 1134
Size: 575 559
Enzymes that do cut:
AarI AcuI AlwNI AvaI BamHI BanI BanII BbsI
BclI BglI Bme1580I BpmI BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoO109I FalI
Hin4I MmeI MscI MslI MspA1I NcoI NspI PflMI
PfoI PpuMI PstI PvuII SapI ScaI SexAI SfcI
SmaI SmlI SphI SrfI StyI TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsiEI BsiWI BspHI BsrDI BsrFI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SspI StuI SwaI TaqII Tth111I XbaI
XhoI XmnI ZraI