(Linear) (Six-base) MAPSORT of: KIBB5425.seq  Check: 9844  from: 1  to: 390



KIBB5425.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     390 
   Size:        280     110
 
AleI CACnn'nnGTG

Cuts at:      0     232     390 
   Size:        232     158
 
AlwNI CAG_nnn'CTG

Cuts at:      0     131     390 
   Size:        131     259
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      92     390 
   Size:         92     298
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     313     390 
   Size:        313      77
 
BglII A'GATC_T

Cuts at:      0      54     203     263     390 
   Size:         54     149      60     127

  Fragments arranged by size: 

                 149     127      60      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     302     390 
   Size:        302      88
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     390 
   Size:        166     224
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     390 
   Size:         52     338
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     163     193     390 
   Size:        163      30     197

  Fragments arranged by size: 

                 197     163      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     286     390 
   Size:        124     162     104

  Fragments arranged by size: 

                 162     124     104
 
BseYI C'CCAG_C

Cuts at:      0      94     390 
   Size:         94     296
 
BsiHKAI G_wGCw'C

Cuts at:      0     194     390 
   Size:        194     196
 
Bsp1286I G_dGCh'C

Cuts at:      0     194     390 
   Size:        194     196
 
BstYI r'GATC_y

Cuts at:      0      54     203     263     390 
   Size:         54     149      60     127

  Fragments arranged by size: 

                 149     127      60      54
 
BtsI GCAGTG_nn'

Cuts at:      0     132     390 
   Size:        132     258
 
EarI CTCTTCn'nnn_

Cuts at:      0     347     390 
   Size:        347      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     280     390 
   Size:        166     114     110

  Fragments arranged by size: 

                 166     114     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     390 
   Size:         14     376
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     163     195     390 
   Size:        163      32     195

  Fragments arranged by size: 

                 195     163      32
 
HincII GTy'rAC

Cuts at:      0     240     390 
   Size:        240     150
 
MslI CAynn'nnrTG

Cuts at:      0     232     390 
   Size:        232     158
 
PfoI T'CCnGG_A

Cuts at:      0     143     390 
   Size:        143     247
 
PpuMI rG'GwC_Cy

Cuts at:      0      14     390 
   Size:         14     376
 
SanDI GG'GwC_CC

Cuts at:      0      14     390 
   Size:         14     376
 
SfcI C'TryA_G

Cuts at:      0     243     390 
   Size:        243     147
 
SmlI C'TyrA_G

Cuts at:      0      67     390 
   Size:         67     323
 
TatI w'GTAC_w

Cuts at:      0     320     390 
   Size:        320      70
 
XbaI T'CTAG_A

Cuts at:      0      57     390 
   Size:         57     333
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     BbsI    BciVI    BglII     BmrI     BpmI
    BpuEI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BstYI     BtsI
     EarI  Eco57MI EcoO109I    Hin4I   HincII     MslI     PfoI    PpuMI
    SanDI     SfcI     SmlI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI