Restriction map for
pF1KB5422
(Linear) (Six-base) MAPSORT of: KIBB5422.seq Check: 1994 from: 1 to: 672
KIBB5422.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 672
Size: 281 391
AleI CACnn'nnGTG
Cuts at: 0 58 672
Size: 58 614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 672
Size: 169 503
AlwNI CAG_nnn'CTG
Cuts at: 0 78 107 415 672
Size: 78 29 308 257
Fragments arranged by size:
308 257 78 29
ApaI G_GGCC'C
Cuts at: 0 630 672
Size: 630 42
ApoI r'AATT_y
Cuts at: 0 346 672
Size: 346 326
AvaI C'yCGr_G
Cuts at: 0 28 329 595 672
Size: 28 301 266 77
Fragments arranged by size:
301 266 77 28
BanII G_rGCy'C
Cuts at: 0 630 656 672
Size: 630 26 16
Fragments arranged by size:
630 26 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 638 672
Size: 604 34 34
Fragments arranged by size:
604 34 34
BglII A'GATC_T
Cuts at: 0 206 672
Size: 206 466
Bme1580I G_kGCm'C
Cuts at: 0 65 630 672
Size: 65 565 42
Fragments arranged by size:
565 65 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 586 672
Size: 189 397 86
Fragments arranged by size:
397 189 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 342 672
Size: 342 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 300 672
Size: 64 236 372
Fragments arranged by size:
372 236 64
Bpu10I CC'TnA_GC
Cuts at: 0 650 672
Size: 650 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 326 505 672
Size: 326 179 167
Fragments arranged by size:
326 179 167
BsaAI yAC'GTr
Cuts at: 0 60 672
Size: 60 612
BsaHI Gr'CG_yC
Cuts at: 0 531 672
Size: 531 141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 497 527 672
Size: 497 30 145
Fragments arranged by size:
497 145 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 229 672
Size: 214 15 443
Fragments arranged by size:
443 214 15
Bsp1286I G_dGCh'C
Cuts at: 0 65 630 656 672
Size: 65 565 26 16
Fragments arranged by size:
565 65 26 16
BsrFI r'CCGG_y
Cuts at: 0 581 672
Size: 581 91
BssSI C'ACGA_G
Cuts at: 0 127 672
Size: 127 545
BstEII G'GTnAC_C
Cuts at: 0 601 672
Size: 601 71
BstYI r'GATC_y
Cuts at: 0 206 321 672
Size: 206 115 351
Fragments arranged by size:
351 206 115
BtgI C'CryG_G
Cuts at: 0 191 672
Size: 191 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 317 495 672
Size: 317 178 177
Fragments arranged by size:
317 178 177
EaeI y'GGCC_r
Cuts at: 0 194 672
Size: 194 478
EarI CTCTTCn'nnn_
Cuts at: 0 257 470 672
Size: 257 213 202
Fragments arranged by size:
257 213 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 281 300 672
Size: 64 217 19 372
Fragments arranged by size:
372 217 64 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 672
Size: 157 515
EcoO109I rG'GnC_Cy
Cuts at: 0 114 441 627 672
Size: 114 327 186 45
Fragments arranged by size:
327 186 114 45
EcoRI G'AATT_C
Cuts at: 0 346 672
Size: 346 326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 643 672
Size: 371 32 240 29
Fragments arranged by size:
371 240 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 672
Size: 540 132
MslI CAynn'nnrTG
Cuts at: 0 58 672
Size: 58 614
MspA1I CmG'CkG
Cuts at: 0 75 400 634 672
Size: 75 325 234 38
Fragments arranged by size:
325 234 75 38
NaeI GCC'GGC
Cuts at: 0 583 672
Size: 583 89
NgoMIV G'CCGG_C
Cuts at: 0 581 672
Size: 581 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 672
Size: 192 480
PmlI CAC'GTG
Cuts at: 0 60 672
Size: 60 612
PpuMI rG'GwC_Cy
Cuts at: 0 114 441 672
Size: 114 327 231
Fragments arranged by size:
327 231 114
PspOMI G'GGCC_C
Cuts at: 0 626 672
Size: 626 46
PstI C_TGCA'G
Cuts at: 0 103 672
Size: 103 569
PvuII CAG'CTG
Cuts at: 0 75 400 672
Size: 75 325 272
Fragments arranged by size:
325 272 75
SapI GCTCTTCn'nnn_
Cuts at: 0 470 672
Size: 470 202
SexAI A'CCwGG_T
Cuts at: 0 359 672
Size: 359 313
SfcI C'TryA_G
Cuts at: 0 99 672
Size: 99 573
SmlI C'TyrA_G
Cuts at: 0 329 672
Size: 329 343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 672
Size: 497 32 143
Fragments arranged by size:
497 143 32
XhoI C'TCGA_G
Cuts at: 0 329 672
Size: 329 343
XmnI GAAnn'nnTTC
Cuts at: 0 208 346 672
Size: 208 138 326
Fragments arranged by size:
326 208 138
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApoI AvaI BanII
BcgI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaXI BsgI Bsp1286I BsrFI BssSI BstEII
BstYI BtgI BtgZI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI Hin4I MmeI MslI MspA1I NaeI NgoMIV PflMI
PmlI PpuMI PspOMI PstI PvuII SapI SexAI SfcI
SmlI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BpuEI BsaBI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XmaI ZraI