Restriction map for
pF1KB5420
(Linear) (Six-base) MAPSORT of: KIBB5420.seq Check: 9041 from: 1 to: 1302
KIBB5420.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 443 516 595 700 738 1045 1148
Size: 75 368 73 79 105 38 307 103
Cuts at: 1148 1266 1302
Size: 118 36
Fragments arranged by size:
368 307 118 105 103 79 75 73
38 36
AfeI AGC'GCT
Cuts at: 0 1207 1302
Size: 1207 95
AhdI GACnn_n'nnGTC
Cuts at: 0 783 1302
Size: 783 519
AleI CACnn'nnGTG
Cuts at: 0 616 1302
Size: 616 686
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 988 1020 1302
Size: 988 32 282
Fragments arranged by size:
988 282 32
ApaLI G'TGCA_C
Cuts at: 0 1164 1302
Size: 1164 138
BanI G'GyrC_C
Cuts at: 0 364 1186 1302
Size: 364 822 116
Fragments arranged by size:
822 364 116
BanII G_rGCy'C
Cuts at: 0 146 1302
Size: 146 1156
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 1302
Size: 688 614
BglII A'GATC_T
Cuts at: 0 29 1302
Size: 29 1273
Bme1580I G_kGCm'C
Cuts at: 0 107 1168 1191 1302
Size: 107 1061 23 111
Fragments arranged by size:
1061 111 107 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 508 856 909 1178 1302
Size: 101 407 348 53 269 124
Fragments arranged by size:
407 348 269 124 101 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 691 1302
Size: 673 18 611
Fragments arranged by size:
673 611 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 1302
Size: 91 1211
BsaHI Gr'CG_yC
Cuts at: 0 1097 1302
Size: 1097 205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 1302
Size: 545 30 727
Fragments arranged by size:
727 545 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1219 1258 1302
Size: 1219 39 44
Fragments arranged by size:
1219 44 39
BseYI C'CCAG_C
Cuts at: 0 799 1248 1302
Size: 799 449 54
Fragments arranged by size:
799 449 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 950 1302
Size: 542 408 352
Fragments arranged by size:
542 408 352
BsiHKAI G_wGCw'C
Cuts at: 0 146 203 1168 1302
Size: 146 57 965 134
Fragments arranged by size:
965 146 134 57
Bsp1286I G_dGCh'C
Cuts at: 0 107 146 203 1168 1191 1302
Size: 107 39 57 965 23 111
Fragments arranged by size:
965 111 107 57 39 23
BspHI T'CATG_A
Cuts at: 0 503 1302
Size: 503 799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 794 1302
Size: 794 508
BsrFI r'CCGG_y
Cuts at: 0 1270 1302
Size: 1270 32
BsrGI T'GTAC_A
Cuts at: 0 836 1302
Size: 836 466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 651 1087 1302
Size: 618 33 436 215
Fragments arranged by size:
618 436 215 33
BstYI r'GATC_y
Cuts at: 0 14 29 879 1302
Size: 14 15 850 423
Fragments arranged by size:
850 423 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 1125 1302
Size: 384 741 177
Fragments arranged by size:
741 384 177
EaeI y'GGCC_r
Cuts at: 0 488 1211 1295 1302
Size: 488 723 84 7
Fragments arranged by size:
723 488 84 7
EarI CTCTTCn'nnn_
Cuts at: 0 76 94 1064 1235 1302
Size: 76 18 970 171 67
Fragments arranged by size:
970 171 76 67 18
EcoICRI GAG'CTC
Cuts at: 0 144 1302
Size: 144 1158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 443 516 595 673 691 700 738
Size: 75 368 73 79 78 18 9 38
Cuts at: 738 1045 1148 1266 1302
Size: 307 103 118 36
Fragments arranged by size:
368 307 118 103 79 78 75 73
38 36 18 9
EcoO109I rG'GnC_Cy
Cuts at: 0 135 381 1302
Size: 135 246 921
Fragments arranged by size:
921 246 135
FspI TGC'GCA
Cuts at: 0 547 1302
Size: 547 755
FspAI rTGC'GCAy
Cuts at: 0 547 1302
Size: 547 755
HaeII r_GCGC'y
Cuts at: 0 1209 1302
Size: 1209 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 769 801 1041 1073 1302
Size: 769 32 240 32 229
Fragments arranged by size:
769 240 229 32 32
HincII GTy'rAC
Cuts at: 0 1032 1302
Size: 1032 270
MscI TGG'CCA
Cuts at: 0 490 1213 1297 1302
Size: 490 723 84 5
Fragments arranged by size:
723 490 84 5
MslI CAynn'nnrTG
Cuts at: 0 616 727 1302
Size: 616 111 575
Fragments arranged by size:
616 575 111
MspA1I CmG'CkG
Cuts at: 0 825 1302
Size: 825 477
NaeI GCC'GGC
Cuts at: 0 1272 1302
Size: 1272 30
NgoMIV G'CCGG_C
Cuts at: 0 1270 1302
Size: 1270 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1091 1302
Size: 945 146 211
Fragments arranged by size:
945 211 146
PfoI T'CCnGG_A
Cuts at: 0 146 650 1302
Size: 146 504 652
Fragments arranged by size:
652 504 146
PpuMI rG'GwC_Cy
Cuts at: 0 381 1302
Size: 381 921
PvuII CAG'CTG
Cuts at: 0 825 1302
Size: 825 477
SacI G_AGCT'C
Cuts at: 0 146 1302
Size: 146 1156
SanDI GG'GwC_CC
Cuts at: 0 381 1302
Size: 381 921
SexAI A'CCwGG_T
Cuts at: 0 1148 1302
Size: 1148 154
SmlI C'TyrA_G
Cuts at: 0 10 1302
Size: 10 1292
StuI AGG'CCT
Cuts at: 0 668 1302
Size: 668 634
StyI C'CwwG_G
Cuts at: 0 986 1292 1302
Size: 986 306 10
Fragments arranged by size:
986 306 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1066 1302
Size: 1066 236
TatI w'GTAC_w
Cuts at: 0 836 1217 1302
Size: 836 381 85
Fragments arranged by size:
836 381 85
Tth111I GACn'n_nGTC
Cuts at: 0 1053 1302
Size: 1053 249
Enzymes that do cut:
AcuI AfeI AhdI AleI AloI ApaLI BanI BanII
BbsI BglII Bme1580I BmrI BpmI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BsrGI BstXI BstYI BtgZI EaeI EarI EcoICRI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I HincII MscI MslI
MspA1I NaeI NgoMIV PflMI PfoI PpuMI PvuII SacI
SanDI SexAI SmlI StuI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AlfI AlwNI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XcmI
XhoI XmaI XmnI ZraI