Restriction map for
pF1KB5413
(Linear) (Six-base) MAPSORT of: KIBB5413.seq Check: 6470 from: 1 to: 639
KIBB5413.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 639
Size: 574 65
AlwNI CAG_nnn'CTG
Cuts at: 0 314 639
Size: 314 325
AvaI C'yCGr_G
Cuts at: 0 408 639
Size: 408 231
BanII G_rGCy'C
Cuts at: 0 449 639
Size: 449 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 51 639
Size: 51 588
BclI T'GATC_A
Cuts at: 0 581 639
Size: 581 58
BlpI GC'TnA_GC
Cuts at: 0 124 639
Size: 124 515
Bme1580I G_kGCm'C
Cuts at: 0 32 639
Size: 32 607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 434 639
Size: 434 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 639
Size: 284 355
BsaWI w'CCGG_w
Cuts at: 0 384 515 639
Size: 384 131 124
Fragments arranged by size:
384 131 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 639
Size: 205 30 404
Fragments arranged by size:
404 205 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 451 639
Size: 99 352 188
Fragments arranged by size:
352 188 99
BseYI C'CCAG_C
Cuts at: 0 436 639
Size: 436 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 639
Size: 304 335
BsiEI CG_ry'CG
Cuts at: 0 590 639
Size: 590 49
BsiHKAI G_wGCw'C
Cuts at: 0 449 639
Size: 449 190
Bsp1286I G_dGCh'C
Cuts at: 0 32 449 639
Size: 32 417 190
Fragments arranged by size:
417 190 32
BspEI T'CCGG_A
Cuts at: 0 515 639
Size: 515 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 639
Size: 128 511
BstYI r'GATC_y
Cuts at: 0 395 639
Size: 395 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 528 639
Size: 528 111
BtsI GCAGTG_nn'
Cuts at: 0 354 533 639
Size: 354 179 106
Fragments arranged by size:
354 179 106
DraIII CAC_nnn'GTG
Cuts at: 0 230 431 639
Size: 230 201 208
Fragments arranged by size:
230 208 201
EaeI y'GGCC_r
Cuts at: 0 305 587 639
Size: 305 282 52
Fragments arranged by size:
305 282 52
EagI C'GGCC_G
Cuts at: 0 587 639
Size: 587 52
EarI CTCTTCn'nnn_
Cuts at: 0 454 639
Size: 454 185
EcoICRI GAG'CTC
Cuts at: 0 447 639
Size: 447 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 574 639
Size: 284 290 65
Fragments arranged by size:
290 284 65
EcoO109I rG'GnC_Cy
Cuts at: 0 487 639
Size: 487 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 639
Size: 205 32 402
Fragments arranged by size:
402 205 32
HincII GTy'rAC
Cuts at: 0 243 639
Size: 243 396
MscI TGG'CCA
Cuts at: 0 307 639
Size: 307 332
MspA1I CmG'CkG
Cuts at: 0 128 218 639
Size: 128 90 421
Fragments arranged by size:
421 128 90
NspI r_CATG'y
Cuts at: 0 207 639
Size: 207 432
PflMI CCAn_nnn'nTGG
Cuts at: 0 314 639
Size: 314 325
PfoI T'CCnGG_A
Cuts at: 0 376 639
Size: 376 263
PpuMI rG'GwC_Cy
Cuts at: 0 487 639
Size: 487 152
PspXI vC'TCGA_Gb
Cuts at: 0 408 639
Size: 408 231
RsrII CG'GwC_CG
Cuts at: 0 213 639
Size: 213 426
SacI G_AGCT'C
Cuts at: 0 449 639
Size: 449 190
SapI GCTCTTCn'nnn_
Cuts at: 0 454 639
Size: 454 185
SmlI C'TyrA_G
Cuts at: 0 408 639
Size: 408 231
StuI AGG'CCT
Cuts at: 0 414 639
Size: 414 225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 639
Size: 234 405
XhoI C'TCGA_G
Cuts at: 0 408 639
Size: 408 231
Enzymes that do cut:
AcuI AlwNI AvaI BanII BbsI BclI BlpI Bme1580I
BplI BpmI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BstYI BtgZI BtsI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII
MscI MspA1I NspI PflMI PfoI PpuMI PspXI RsrII
SacI SapI SmlI StuI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XmaI XmnI ZraI