Restriction map for
pF1KB5412
(Linear) (Six-base) MAPSORT of: KIBB5412.seq Check: 4065 from: 1 to: 1056
KIBB5412.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 1056
Size: 574 32 450
Fragments arranged by size:
574 450 32
AlwNI CAG_nnn'CTG
Cuts at: 0 229 997 1056
Size: 229 768 59
Fragments arranged by size:
768 229 59
ApoI r'AATT_y
Cuts at: 0 328 416 444 794 1056
Size: 328 88 28 350 262
Fragments arranged by size:
350 328 262 88 28
AvaI C'yCGr_G
Cuts at: 0 79 1056
Size: 79 977
BanII G_rGCy'C
Cuts at: 0 149 1056
Size: 149 907
BclI T'GATC_A
Cuts at: 0 492 1056
Size: 492 564
BglII A'GATC_T
Cuts at: 0 864 1056
Size: 864 192
BlpI GC'TnA_GC
Cuts at: 0 541 1056
Size: 541 515
Bme1580I G_kGCm'C
Cuts at: 0 374 1056
Size: 374 682
BmrI ACTGGGnnnn_n'
Cuts at: 0 1026 1056
Size: 1026 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 615 1056
Size: 508 107 441
Fragments arranged by size:
508 441 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 761 1056
Size: 33 728 295
Fragments arranged by size:
728 295 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 131 1056
Size: 131 925
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 1056
Size: 601 30 425
Fragments arranged by size:
601 425 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 133 139 697 925 1056
Size: 130 3 6 558 228 131
Fragments arranged by size:
558 228 131 130 6 3
BseYI C'CCAG_C
Cuts at: 0 638 1056
Size: 638 418
BsiHKAI G_wGCw'C
Cuts at: 0 149 1056
Size: 149 907
BsmBI CGTCTCn'nnnn_
Cuts at: 0 88 621 1056
Size: 88 533 435
Fragments arranged by size:
533 435 88
Bsp1286I G_dGCh'C
Cuts at: 0 149 374 1056
Size: 149 225 682
Fragments arranged by size:
682 225 149
BsrDI GCAATG_nn'
Cuts at: 0 699 1056
Size: 699 357
BsrFI r'CCGG_y
Cuts at: 0 39 1056
Size: 39 1017
BstAPI GCAn_nnn'nTGC
Cuts at: 0 199 711 891 1056
Size: 199 512 180 165
Fragments arranged by size:
512 199 180 165
BstYI r'GATC_y
Cuts at: 0 864 1056
Size: 864 192
BtgI C'CryG_G
Cuts at: 0 376 506 1056
Size: 376 130 550
Fragments arranged by size:
550 376 130
EarI CTCTTCn'nnn_
Cuts at: 0 332 682 923 1056
Size: 332 350 241 133
Fragments arranged by size:
350 332 241 133
EcoICRI GAG'CTC
Cuts at: 0 147 1056
Size: 147 909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 615 1056
Size: 508 107 441
Fragments arranged by size:
508 441 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 856 1056
Size: 154 702 200
Fragments arranged by size:
702 200 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 545 577 1056
Size: 73 32 440 32 479
Fragments arranged by size:
479 440 73 32 32
HincII GTy'rAC
Cuts at: 0 384 1056
Size: 384 672
MspA1I CmG'CkG
Cuts at: 0 367 433 717 1056
Size: 367 66 284 339
Fragments arranged by size:
367 339 284 66
NcoI C'CATG_G
Cuts at: 0 376 1056
Size: 376 680
PfoI T'CCnGG_A
Cuts at: 0 524 1056
Size: 524 532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 592 624 1056
Size: 592 32 432
Fragments arranged by size:
592 432 32
PstI C_TGCA'G
Cuts at: 0 128 942 962 1056
Size: 128 814 20 94
Fragments arranged by size:
814 128 94 20
PvuII CAG'CTG
Cuts at: 0 367 433 1056
Size: 367 66 623
Fragments arranged by size:
623 367 66
SacI G_AGCT'C
Cuts at: 0 149 1056
Size: 149 907
SapI GCTCTTCn'nnn_
Cuts at: 0 332 682 1056
Size: 332 350 374
Fragments arranged by size:
374 350 332
SfcI C'TryA_G
Cuts at: 0 124 938 958 1056
Size: 124 814 20 98
Fragments arranged by size:
814 124 98 20
SmlI C'TyrA_G
Cuts at: 0 12 776 1056
Size: 12 764 280
Fragments arranged by size:
764 280 12
SpeI A'CTAG_T
Cuts at: 0 888 1056
Size: 888 168
StyI C'CwwG_G
Cuts at: 0 376 1056
Size: 376 680
XmnI GAAnn'nnTTC
Cuts at: 0 404 1056
Size: 404 652
Enzymes that do cut:
AloI AlwNI ApoI AvaI BanII BclI BglII BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BstAPI BstYI BtgI
EarI EcoICRI Eco57MI EcoNI Hin4I HincII MspA1I NcoI
PfoI PsrI PstI PvuII SacI SapI SfcI SmlI
SpeI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI