Restriction map for
pF1KB5411
(Linear) (Six-base) MAPSORT of: KIBB5411.seq Check: 4662 from: 1 to: 1785
KIBB5411.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 335 643 1333 1381 1785
Size: 15 320 308 690 48 404
Fragments arranged by size:
690 404 320 308 48 15
AhdI GACnn_n'nnGTC
Cuts at: 0 1512 1785
Size: 1512 273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 709 1618 1785
Size: 709 909 167
Fragments arranged by size:
909 709 167
AlwNI CAG_nnn'CTG
Cuts at: 0 639 1213 1371 1785
Size: 639 574 158 414
Fragments arranged by size:
639 574 414 158
ApoI r'AATT_y
Cuts at: 0 295 1785
Size: 295 1490
AvaI C'yCGr_G
Cuts at: 0 1019 1760 1785
Size: 1019 741 25
Fragments arranged by size:
1019 741 25
BanII G_rGCy'C
Cuts at: 0 167 1784 1785
Size: 167 1617 1
Fragments arranged by size:
1617 167 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 837 1785
Size: 837 948
BbvCI CC'TCA_GC
Cuts at: 0 1322 1785
Size: 1322 463
BglI GCCn_nnn'nGGC
Cuts at: 0 439 1201 1210 1348 1785
Size: 439 762 9 138 437
Fragments arranged by size:
762 439 437 138 9
BglII A'GATC_T
Cuts at: 0 389 758 1785
Size: 389 369 1027
Fragments arranged by size:
1027 389 369
BlpI GC'TnA_GC
Cuts at: 0 1214 1785
Size: 1214 571
Bme1580I G_kGCm'C
Cuts at: 0 688 1785
Size: 688 1097
BmrI ACTGGGnnnn_n'
Cuts at: 0 458 1785
Size: 458 1327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 874 1438 1785
Size: 661 213 564 347
Fragments arranged by size:
661 564 347 213
Bpu10I CC'TnA_GC
Cuts at: 0 632 1322 1785
Size: 632 690 463
Fragments arranged by size:
690 632 463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1609 1785
Size: 25 1584 176
Fragments arranged by size:
1584 176 25
BsaAI yAC'GTr
Cuts at: 0 1439 1785
Size: 1439 346
BsaHI Gr'CG_yC
Cuts at: 0 89 1785
Size: 89 1696
BsaWI w'CCGG_w
Cuts at: 0 540 671 1785
Size: 540 131 1114
Fragments arranged by size:
1114 540 131
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 568 849 939 994 1054 1086 1147
Size: 334 234 281 90 55 60 32 61
Cuts at: 1147 1189 1192 1273 1276 1339 1350 1785
Size: 42 3 81 3 63 11 435
Fragments arranged by size:
435 334 281 234 90 81 63 61
60 55 42 32 11 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1666 1785
Size: 1666 119
BsiHKAI G_wGCw'C
Cuts at: 0 419 584 1784 1785
Size: 419 165 1200 1
Fragments arranged by size:
1200 419 165 1
BsmI GAATG_Cn'
Cuts at: 0 315 1785
Size: 315 1470
Bsp1286I G_dGCh'C
Cuts at: 0 167 419 584 688 1784 1785
Size: 167 252 165 104 1096 1
Fragments arranged by size:
1096 252 167 165 104 1
BspEI T'CCGG_A
Cuts at: 0 540 671 1785
Size: 540 131 1114
Fragments arranged by size:
1114 540 131
BspHI T'CATG_A
Cuts at: 0 249 1785
Size: 249 1536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1379 1785
Size: 1379 406
BsrBI CCG'CTC
Cuts at: 0 1005 1785
Size: 1005 780
BssSI C'ACGA_G
Cuts at: 0 1053 1785
Size: 1053 732
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1224 1287 1371 1785
Size: 1224 63 84 414
Fragments arranged by size:
1224 414 84 63
BstYI r'GATC_y
Cuts at: 0 389 758 1785
Size: 389 369 1027
Fragments arranged by size:
1027 389 369
Bsu36I CC'TnA_GG
Cuts at: 0 404 1785
Size: 404 1381
BtgI C'CryG_G
Cuts at: 0 83 769 1553 1785
Size: 83 686 784 232
Fragments arranged by size:
784 686 232 83
EaeI y'GGCC_r
Cuts at: 0 2 290 1106 1785
Size: 2 288 816 679
Fragments arranged by size:
816 679 288 2
EarI CTCTTCn'nnn_
Cuts at: 0 311 1392 1772 1785
Size: 311 1081 380 13
Fragments arranged by size:
1081 380 311 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1405 1785
Size: 1405 380
EcoICRI GAG'CTC
Cuts at: 0 1782 1785
Size: 1782 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 335 643 661 874 1333 1381 1438
Size: 15 320 308 18 213 459 48 57
Cuts at: 1438 1785
Size: 347
Fragments arranged by size:
459 347 320 308 213 57 48 18
15
EcoO109I rG'GnC_Cy
Cuts at: 0 132 636 1368 1785
Size: 132 504 732 417
Fragments arranged by size:
732 504 417 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 153 780 1758 1785
Size: 153 627 978 27
Fragments arranged by size:
978 627 153 27
HaeII r_GCGC'y
Cuts at: 0 1014 1272 1754 1785
Size: 1014 258 482 31
Fragments arranged by size:
1014 482 258 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1498 1530 1584 1616 1785
Size: 1498 32 54 32 169
Fragments arranged by size:
1498 169 54 32 32
HindIII A'AGCT_T
Cuts at: 0 865 1488 1785
Size: 865 623 297
Fragments arranged by size:
865 623 297
MfeI C'AATT_G
Cuts at: 0 105 1785
Size: 105 1680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1721 1785
Size: 1046 675 64
Fragments arranged by size:
1046 675 64
MscI TGG'CCA
Cuts at: 0 292 1108 1785
Size: 292 816 677
Fragments arranged by size:
816 677 292
MslI CAynn'nnrTG
Cuts at: 0 728 1785
Size: 728 1057
MspA1I CmG'CkG
Cuts at: 0 1141 1359 1785
Size: 1141 218 426
Fragments arranged by size:
1141 426 218
NcoI C'CATG_G
Cuts at: 0 83 1553 1785
Size: 83 1470 232
Fragments arranged by size:
1470 232 83
PasI CC'CwG_GG
Cuts at: 0 714 1785
Size: 714 1071
PfoI T'CCnGG_A
Cuts at: 0 1415 1785
Size: 1415 370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1785
Size: 85 32 1668
Fragments arranged by size:
1668 85 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 636 1368 1785
Size: 132 504 732 417
Fragments arranged by size:
732 504 417 132
PstI C_TGCA'G
Cuts at: 0 194 1085 1376 1607 1785
Size: 194 891 291 231 178
Fragments arranged by size:
891 291 231 194 178
PvuII CAG'CTG
Cuts at: 0 1141 1359 1785
Size: 1141 218 426
Fragments arranged by size:
1141 426 218
SacI G_AGCT'C
Cuts at: 0 1784 1785
Size: 1784 1
SapI GCTCTTCn'nnn_
Cuts at: 0 1392 1772 1785
Size: 1392 380 13
Fragments arranged by size:
1392 380 13
SbfI CC_TGCA'GG
Cuts at: 0 1376 1785
Size: 1376 409
SexAI A'CCwGG_T
Cuts at: 0 175 1785
Size: 175 1610
SfcI C'TryA_G
Cuts at: 0 190 1081 1372 1603 1785
Size: 190 891 291 231 182
Fragments arranged by size:
891 291 231 190 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1201 1785
Size: 1201 584
SmlI C'TyrA_G
Cuts at: 0 40 1019 1624 1785
Size: 40 979 605 161
Fragments arranged by size:
979 605 161 40
StuI AGG'CCT
Cuts at: 0 1642 1785
Size: 1642 143
StyI C'CwwG_G
Cuts at: 0 83 1553 1785
Size: 83 1470 232
Fragments arranged by size:
1470 232 83
TatI w'GTAC_w
Cuts at: 0 194 1785
Size: 194 1591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 445 1761 1785
Size: 445 1316 24
Fragments arranged by size:
1316 445 24
XhoI C'TCGA_G
Cuts at: 0 1019 1785
Size: 1019 766
XmnI GAAnn'nnTTC
Cuts at: 0 503 1785
Size: 503 1282
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApoI AvaI BanII BbsI
BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BssSI BstAPI BstYI
Bsu36I BtgI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NcoI PasI PfoI PpiI PpuMI PstI PvuII
SacI SapI SbfI SexAI SfcI SfiI SmlI StuI
StyI TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BmgBI BmtI BplI BsaI BsaBI BsaXI BseYI
BsiEI BsiWI BsmBI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XmaI ZraI