Restriction map for
pF1KB5406
(Linear) (Six-base) MAPSORT of: KIBB5406.seq Check: 1975 from: 1 to: 993
KIBB5406.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 156 993
Size: 156 837
AccI GT'mk_AC
Cuts at: 0 85 993
Size: 85 908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 538 993
Size: 2 536 455
Fragments arranged by size:
536 455 2
AlwNI CAG_nnn'CTG
Cuts at: 0 963 993
Size: 963 30
AseI AT'TA_AT
Cuts at: 0 433 993
Size: 433 560
BanI G'GyrC_C
Cuts at: 0 595 993
Size: 595 398
BglII A'GATC_T
Cuts at: 0 314 993
Size: 314 679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 874 993
Size: 349 525 119
Fragments arranged by size:
525 349 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 814 993
Size: 38 776 179
Fragments arranged by size:
776 179 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 850 993
Size: 52 798 143
Fragments arranged by size:
798 143 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 706 736 993
Size: 706 30 257
Fragments arranged by size:
706 257 30
BseYI C'CCAG_C
Cuts at: 0 30 993
Size: 30 963
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 993
Size: 268 725
BsiHKAI G_wGCw'C
Cuts at: 0 971 993
Size: 971 22
BsmI GAATG_Cn'
Cuts at: 0 512 993
Size: 512 481
Bsp1286I G_dGCh'C
Cuts at: 0 971 993
Size: 971 22
BspHI T'CATG_A
Cuts at: 0 95 993
Size: 95 898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 848 993
Size: 156 692 145
Fragments arranged by size:
692 156 145
BsrGI T'GTAC_A
Cuts at: 0 491 660 993
Size: 491 169 333
Fragments arranged by size:
491 333 169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 993
Size: 576 417
BstYI r'GATC_y
Cuts at: 0 314 446 993
Size: 314 132 547
Fragments arranged by size:
547 314 132
BstZ17I GTA'TAC
Cuts at: 0 86 993
Size: 86 907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 91 993
Size: 91 902
ClaI AT'CG_AT
Cuts at: 0 804 993
Size: 804 189
DraI TTT'AAA
Cuts at: 0 378 993
Size: 378 615
EarI CTCTTCn'nnn_
Cuts at: 0 432 575 993
Size: 432 143 418
Fragments arranged by size:
432 418 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 349 538 874 993
Size: 2 347 189 336 119
Fragments arranged by size:
347 336 189 119 2
EcoO109I rG'GnC_Cy
Cuts at: 0 960 993
Size: 960 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 460 492 550 582 706 738
Size: 409 32 19 32 58 32 124 32
Cuts at: 738 993
Size: 255
Fragments arranged by size:
409 255 124 58 32 32 32 32
19
HindIII A'AGCT_T
Cuts at: 0 176 993
Size: 176 817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 993
Size: 95 898
MspA1I CmG'CkG
Cuts at: 0 34 993
Size: 34 959
NspI r_CATG'y
Cuts at: 0 255 993
Size: 255 738
PfoI T'CCnGG_A
Cuts at: 0 365 993
Size: 365 628
PstI C_TGCA'G
Cuts at: 0 167 959 993
Size: 167 792 34
Fragments arranged by size:
792 167 34
PvuII CAG'CTG
Cuts at: 0 34 993
Size: 34 959
SapI GCTCTTCn'nnn_
Cuts at: 0 575 993
Size: 575 418
SbfI CC_TGCA'GG
Cuts at: 0 167 959 993
Size: 167 792 34
Fragments arranged by size:
792 167 34
SfcI C'TryA_G
Cuts at: 0 163 586 955 993
Size: 163 423 369 38
Fragments arranged by size:
423 369 163 38
SmlI C'TyrA_G
Cuts at: 0 53 829 993
Size: 53 776 164
Fragments arranged by size:
776 164 53
SphI G_CATG'C
Cuts at: 0 255 993
Size: 255 738
SspI AAT'ATT
Cuts at: 0 609 993
Size: 609 384
StyI C'CwwG_G
Cuts at: 0 305 993
Size: 305 688
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 517 993
Size: 517 476
TatI w'GTAC_w
Cuts at: 0 491 660 993
Size: 491 169 333
Fragments arranged by size:
491 333 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 993
Size: 612 381
Enzymes that do cut:
AarI AccI AcuI AlwNI AseI BanI BglII BpmI
BpuEI BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BstXI BstYI BstZ17I BtgZI ClaI
DraI EarI Eco57MI EcoO109I Hin4I HindIII MmeI MspA1I
NspI PfoI PstI PvuII SapI SbfI SfcI SmlI
SphI SspI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI