Restriction map for
pF1KB5397
(Linear) (Six-base) MAPSORT of: KIBB5397.seq Check: 4889 from: 1 to: 927
KIBB5397.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 814 927
Size: 385 429 113
Fragments arranged by size:
429 385 113
AvaI C'yCGr_G
Cuts at: 0 57 927
Size: 57 870
BamHI G'GATC_C
Cuts at: 0 892 927
Size: 892 35
BanII G_rGCy'C
Cuts at: 0 315 927
Size: 315 612
BbvCI CC'TCA_GC
Cuts at: 0 13 927
Size: 13 914
BciVI GTATCCnnnnn_n'
Cuts at: 0 448 927
Size: 448 479
BglI GCCn_nnn'nGGC
Cuts at: 0 319 927
Size: 319 608
Bme1580I G_kGCm'C
Cuts at: 0 212 379 927
Size: 212 167 548
Fragments arranged by size:
548 212 167
BmgBI CAC'GTC
Cuts at: 0 438 446 927
Size: 438 8 481
Fragments arranged by size:
481 438 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 498 927
Size: 498 429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 696 927
Size: 696 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 640 927
Size: 553 87 287
Fragments arranged by size:
553 287 87
Bpu10I CC'TnA_GC
Cuts at: 0 13 927
Size: 13 914
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 378 408 532 562 927
Size: 378 30 124 30 365
Fragments arranged by size:
378 365 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 404 927
Size: 19 385 523
Fragments arranged by size:
523 385 19
BseYI C'CCAG_C
Cuts at: 0 24 314 630 702 927
Size: 24 290 316 72 225
Fragments arranged by size:
316 290 225 72 24
BsiHKAI G_wGCw'C
Cuts at: 0 269 927
Size: 269 658
BsmI GAATG_Cn'
Cuts at: 0 515 927
Size: 515 412
Bsp1286I G_dGCh'C
Cuts at: 0 212 269 315 379 927
Size: 212 57 46 64 548
Fragments arranged by size:
548 212 64 57 46
BspHI T'CATG_A
Cuts at: 0 908 927
Size: 908 19
BsrFI r'CCGG_y
Cuts at: 0 687 927
Size: 687 240
BstYI r'GATC_y
Cuts at: 0 596 892 927
Size: 596 296 35
Fragments arranged by size:
596 296 35
BtgI C'CryG_G
Cuts at: 0 47 642 789 927
Size: 47 595 147 138
Fragments arranged by size:
595 147 138 47
EaeI y'GGCC_r
Cuts at: 0 320 717 927
Size: 320 397 210
Fragments arranged by size:
397 320 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 151 771 927
Size: 151 620 156
Fragments arranged by size:
620 156 151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 553 640 814 927
Size: 385 168 87 174 113
Fragments arranged by size:
385 174 168 113 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 367 463 927
Size: 367 96 464
Fragments arranged by size:
464 367 96
EcoO109I rG'GnC_Cy
Cuts at: 0 564 648 807 927
Size: 564 84 159 120
Fragments arranged by size:
564 159 120 84
HaeII r_GCGC'y
Cuts at: 0 828 927
Size: 828 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 927
Size: 532 32 363
Fragments arranged by size:
532 363 32
HindIII A'AGCT_T
Cuts at: 0 304 346 927
Size: 304 42 581
Fragments arranged by size:
581 304 42
MslI CAynn'nnrTG
Cuts at: 0 274 927
Size: 274 653
MspA1I CmG'CkG
Cuts at: 0 318 927
Size: 318 609
NspI r_CATG'y
Cuts at: 0 753 927
Size: 753 174
PpuMI rG'GwC_Cy
Cuts at: 0 648 927
Size: 648 279
PstI C_TGCA'G
Cuts at: 0 34 590 927
Size: 34 556 337
Fragments arranged by size:
556 337 34
PvuII CAG'CTG
Cuts at: 0 318 927
Size: 318 609
SfcI C'TryA_G
Cuts at: 0 30 586 927
Size: 30 556 341
Fragments arranged by size:
556 341 30
StyI C'CwwG_G
Cuts at: 0 131 803 927
Size: 131 672 124
Fragments arranged by size:
672 131 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 378 410 422 454 927
Size: 378 32 12 32 473
Fragments arranged by size:
473 378 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 0 490 927
Size: 490 437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 927
Size: 225 702
Enzymes that do cut:
AcuI AvaI BamHI BanII BbvCI BciVI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BsrFI BstYI BtgI EaeI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MslI
MspA1I NspI PpuMI PstI PvuII SfcI StyI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbsI BcgI BclI BfrBI BglII BlpI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI XbaI XhoI XmaI
XmnI ZraI