Restriction map for
pF1KB5389
(Linear) (Six-base) MAPSORT of: KIBB5389.seq Check: 6413 from: 1 to: 1437
KIBB5389.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AclI AA'CG_TT
Cuts at: 0 878 1437
Size: 878 559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 910 1437
Size: 595 315 527
Fragments arranged by size:
595 527 315
AfeI AGC'GCT
Cuts at: 0 1132 1437
Size: 1132 305
AflII C'TTAA_G
Cuts at: 0 73 1437
Size: 73 1364
AflIII A'CryG_T
Cuts at: 0 1172 1437
Size: 1172 265
AleI CACnn'nnGTG
Cuts at: 0 1171 1437
Size: 1171 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 59 1437
Size: 59 1378
AlwNI CAG_nnn'CTG
Cuts at: 0 800 810 1437
Size: 800 10 627
Fragments arranged by size:
800 627 10
ApaI G_GGCC'C
Cuts at: 0 21 1437
Size: 21 1416
ApoI r'AATT_y
Cuts at: 0 559 1437
Size: 559 878
BamHI G'GATC_C
Cuts at: 0 246 1437
Size: 246 1191
BanI G'GyrC_C
Cuts at: 0 646 1437
Size: 646 791
BanII G_rGCy'C
Cuts at: 0 21 389 521 1058 1437
Size: 21 368 132 537 379
Fragments arranged by size:
537 379 368 132 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 839 1437
Size: 145 694 598
Fragments arranged by size:
694 598 145
BclI T'GATC_A
Cuts at: 0 194 1437
Size: 194 1243
BglI GCCn_nnn'nGGC
Cuts at: 0 1298 1437
Size: 1298 139
BlpI GC'TnA_GC
Cuts at: 0 383 457 1437
Size: 383 74 980
Fragments arranged by size:
980 383 74
Bme1580I G_kGCm'C
Cuts at: 0 21 1182 1437
Size: 21 1161 255
Fragments arranged by size:
1161 255 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 168 483 1437
Size: 168 315 954
Fragments arranged by size:
954 315 168
BmtI G_CTAG'C
Cuts at: 0 583 1437
Size: 583 854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 986 1437
Size: 667 319 451
Fragments arranged by size:
667 451 319
BsaI GGTCTCn'nnnn_
Cuts at: 0 898 1255 1437
Size: 898 357 182
Fragments arranged by size:
898 357 182
BsaWI w'CCGG_w
Cuts at: 0 1274 1437
Size: 1274 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 293 323 658 688 1437
Size: 293 30 335 30 749
Fragments arranged by size:
749 335 293 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 960 1255 1437
Size: 960 295 182
Fragments arranged by size:
960 295 182
BsiHKAI G_wGCw'C
Cuts at: 0 521 676 1058 1437
Size: 521 155 382 379
Fragments arranged by size:
521 382 379 155
BsmBI CGTCTCn'nnnn_
Cuts at: 0 944 1437
Size: 944 493
Bsp1286I G_dGCh'C
Cuts at: 0 21 389 521 676 1058 1182 1437
Size: 21 368 132 155 382 124 255
Fragments arranged by size:
382 368 255 155 132 124 21
BspHI T'CATG_A
Cuts at: 0 737 1437
Size: 737 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 480 1277 1437
Size: 480 797 160
Fragments arranged by size:
797 480 160
BsrDI GCAATG_nn'
Cuts at: 0 364 995 1437
Size: 364 631 442
Fragments arranged by size:
631 442 364
BstEII G'GTnAC_C
Cuts at: 0 1161 1437
Size: 1161 276
BstYI r'GATC_y
Cuts at: 0 246 1437
Size: 246 1191
BtgI C'CryG_G
Cuts at: 0 326 1182 1232 1437
Size: 326 856 50 205
Fragments arranged by size:
856 326 205 50
BtsI GCAGTG_nn'
Cuts at: 0 1127 1322 1437
Size: 1127 195 115
Fragments arranged by size:
1127 195 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 209 244 1437
Size: 209 35 1193
Fragments arranged by size:
1193 209 35
DraIII CAC_nnn'GTG
Cuts at: 0 1077 1437
Size: 1077 360
EaeI y'GGCC_r
Cuts at: 0 2 449 1437
Size: 2 447 988
Fragments arranged by size:
988 447 2
EarI CTCTTCn'nnn_
Cuts at: 0 74 146 278 896 1437
Size: 74 72 132 618 541
Fragments arranged by size:
618 541 132 74 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 810 1437
Size: 810 627
EcoICRI GAG'CTC
Cuts at: 0 519 1056 1437
Size: 519 537 381
Fragments arranged by size:
537 519 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 910 1437
Size: 595 315 527
Fragments arranged by size:
595 527 315
EcoO109I rG'GnC_Cy
Cuts at: 0 18 1308 1437
Size: 18 1290 129
Fragments arranged by size:
1290 129 18
HaeII r_GCGC'y
Cuts at: 0 1134 1437
Size: 1134 303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 658 690 732 764 1437
Size: 238 32 388 32 42 32 673
Fragments arranged by size:
673 388 238 42 32 32 32
MscI TGG'CCA
Cuts at: 0 4 451 1437
Size: 4 447 986
Fragments arranged by size:
986 447 4
MslI CAynn'nnrTG
Cuts at: 0 1171 1437
Size: 1171 266
MspA1I CmG'CkG
Cuts at: 0 243 436 1184 1437
Size: 243 193 748 253
Fragments arranged by size:
748 253 243 193
NcoI C'CATG_G
Cuts at: 0 326 1437
Size: 326 1111
NheI G'CTAG_C
Cuts at: 0 579 1437
Size: 579 858
PasI CC'CwG_GG
Cuts at: 0 652 1437
Size: 652 785
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 1077 1437
Size: 861 216 360
Fragments arranged by size:
861 360 216
PfoI T'CCnGG_A
Cuts at: 0 1364 1437
Size: 1364 73
PspOMI G'GGCC_C
Cuts at: 0 17 1437
Size: 17 1420
PstI C_TGCA'G
Cuts at: 0 242 1122 1437
Size: 242 880 315
Fragments arranged by size:
880 315 242
PvuII CAG'CTG
Cuts at: 0 436 1437
Size: 436 1001
SacI G_AGCT'C
Cuts at: 0 521 1058 1437
Size: 521 537 379
Fragments arranged by size:
537 521 379
SacII CC_GC'GG
Cuts at: 0 1185 1437
Size: 1185 252
SapI GCTCTTCn'nnn_
Cuts at: 0 278 1437
Size: 278 1159
ScaI AGT'ACT
Cuts at: 0 928 1437
Size: 928 509
SexAI A'CCwGG_T
Cuts at: 0 1073 1437
Size: 1073 364
SfcI C'TryA_G
Cuts at: 0 238 1118 1437
Size: 238 880 319
Fragments arranged by size:
880 319 238
SmlI C'TyrA_G
Cuts at: 0 73 682 1001 1437
Size: 73 609 319 436
Fragments arranged by size:
609 436 319 73
StuI AGG'CCT
Cuts at: 0 967 1437
Size: 967 470
StyI C'CwwG_G
Cuts at: 0 312 326 1437
Size: 312 14 1111
Fragments arranged by size:
1111 312 14
TatI w'GTAC_w
Cuts at: 0 263 926 1437
Size: 263 663 511
Fragments arranged by size:
663 511 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 293 325 1437
Size: 293 32 1112
Fragments arranged by size:
1112 293 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 415 1437
Size: 415 1022
XmnI GAAnn'nnTTC
Cuts at: 0 287 1437
Size: 287 1150
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AleI AlfI AlwNI
ApaI ApoI BamHI BanI BanII BbsI BclI BglI
BlpI Bme1580I BmrI BmtI BpuEI BsaI BsaWI BsaXI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BstEII
BstYI BtgI BtsI CspCI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I MscI MslI MspA1I
NcoI NheI PasI PflMI PfoI PspOMI PstI PvuII
SacI SacII SapI ScaI SexAI SfcI SmlI StuI
StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BciVI BfrBI BglII BmgBI BplI BpmI Bpu10I
BsaAI BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI XmaI ZraI