Restriction map for
pF1KB5387
(Linear) (Six-base) MAPSORT of: KIBB5387.seq Check: 4714 from: 1 to: 1629
KIBB5387.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AfeI AGC'GCT
Cuts at: 0 1519 1629
Size: 1519 110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1616 1629
Size: 1616 13
AlwNI CAG_nnn'CTG
Cuts at: 0 642 1629
Size: 642 987
ApaI G_GGCC'C
Cuts at: 0 771 1629
Size: 771 858
ApaLI G'TGCA_C
Cuts at: 0 1090 1629
Size: 1090 539
ApoI r'AATT_y
Cuts at: 0 163 1629
Size: 163 1466
AvaI C'yCGr_G
Cuts at: 0 569 1629
Size: 569 1060
BamHI G'GATC_C
Cuts at: 0 615 1629
Size: 615 1014
BanI G'GyrC_C
Cuts at: 0 201 663 1188 1629
Size: 201 462 525 441
Fragments arranged by size:
525 462 441 201
BanII G_rGCy'C
Cuts at: 0 151 428 697 771 1583 1629
Size: 151 277 269 74 812 46
Fragments arranged by size:
812 277 269 151 74 46
BbeI G_GCGC'C
Cuts at: 0 667 1629
Size: 667 962
BbvCI CC'TCA_GC
Cuts at: 0 442 824 1629
Size: 442 382 805
Fragments arranged by size:
805 442 382
BclI T'GATC_A
Cuts at: 0 80 195 1629
Size: 80 115 1434
Fragments arranged by size:
1434 115 80
Bme1580I G_kGCm'C
Cuts at: 0 91 771 1094 1629
Size: 91 680 323 535
Fragments arranged by size:
680 535 323 91
BmgBI CAC'GTC
Cuts at: 0 282 1324 1629
Size: 282 1042 305
Fragments arranged by size:
1042 305 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 1113 1542 1629
Size: 329 784 429 87
Fragments arranged by size:
784 429 329 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 293 440 994 1629
Size: 135 158 147 554 635
Fragments arranged by size:
635 554 158 147 135
Bpu10I CC'TnA_GC
Cuts at: 0 117 442 824 1629
Size: 117 325 382 805
Fragments arranged by size:
805 382 325 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 269 1431 1482 1629
Size: 34 235 1162 51 147
Fragments arranged by size:
1162 235 147 51 34
BsaAI yAC'GTr
Cuts at: 0 1227 1629
Size: 1227 402
BsaHI Gr'CG_yC
Cuts at: 0 664 1629
Size: 664 965
BsaWI w'CCGG_w
Cuts at: 0 1496 1629
Size: 1496 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 521 551 1629
Size: 266 30 225 30 1078
Fragments arranged by size:
1078 266 225 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 472 557 687 1012 1591 1629
Size: 451 21 85 130 325 579 38
Fragments arranged by size:
579 451 325 130 85 38 21
BseYI C'CCAG_C
Cuts at: 0 266 1073 1629
Size: 266 807 556
Fragments arranged by size:
807 556 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1629
Size: 983 646
BsiEI CG_ry'CG
Cuts at: 0 834 1050 1629
Size: 834 216 579
Fragments arranged by size:
834 579 216
BsiHKAI G_wGCw'C
Cuts at: 0 428 1094 1583 1629
Size: 428 666 489 46
Fragments arranged by size:
666 489 428 46
BsmI GAATG_Cn'
Cuts at: 0 890 1629
Size: 890 739
Bsp1286I G_dGCh'C
Cuts at: 0 91 151 428 697 771 1094 1583 1629
Size: 91 60 277 269 74 323 489 46
Fragments arranged by size:
489 323 277 269 91 74 60 46
BspHI T'CATG_A
Cuts at: 0 1145 1629
Size: 1145 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1002 1584 1629
Size: 1002 582 45
Fragments arranged by size:
1002 582 45
BsrBI CCG'CTC
Cuts at: 0 116 1629
Size: 116 1513
BsrDI GCAATG_nn'
Cuts at: 0 875 1629
Size: 875 754
BsrFI r'CCGG_y
Cuts at: 0 886 1040 1103 1629
Size: 886 154 63 526
Fragments arranged by size:
886 526 154 63
BssSI C'ACGA_G
Cuts at: 0 847 1529 1629
Size: 847 682 100
Fragments arranged by size:
847 682 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1317 1629
Size: 1317 312
BstYI r'GATC_y
Cuts at: 0 615 1629
Size: 615 1014
Bsu36I CC'TnA_GG
Cuts at: 0 699 1629
Size: 699 930
BtgI C'CryG_G
Cuts at: 0 1184 1629
Size: 1184 445
BtsI GCAGTG_nn'
Cuts at: 0 529 1000 1310 1629
Size: 529 471 310 319
Fragments arranged by size:
529 471 319 310
EaeI y'GGCC_r
Cuts at: 0 8 626 1629
Size: 8 618 1003
Fragments arranged by size:
1003 618 8
EarI CTCTTCn'nnn_
Cuts at: 0 1406 1629
Size: 1406 223
EcoICRI GAG'CTC
Cuts at: 0 426 1581 1629
Size: 426 1155 48
Fragments arranged by size:
1155 426 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 293 440 994 1629
Size: 135 158 147 554 635
Fragments arranged by size:
635 554 158 147 135
EcoO109I rG'GnC_Cy
Cuts at: 0 767 768 1629
Size: 767 1 861
Fragments arranged by size:
861 767 1
FspI TGC'GCA
Cuts at: 0 316 1629
Size: 316 1313
HaeII r_GCGC'y
Cuts at: 0 667 954 1521 1629
Size: 667 287 567 108
Fragments arranged by size:
667 567 287 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 266 298 394 426 600 632
Size: 45 32 189 32 96 32 174 32
Cuts at: 632 1629
Size: 997
Fragments arranged by size:
997 189 174 96 45 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 663 1629
Size: 663 966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1629
Size: 351 1278
MscI TGG'CCA
Cuts at: 0 10 628 1629
Size: 10 618 1001
Fragments arranged by size:
1001 618 10
MslI CAynn'nnrTG
Cuts at: 0 70 515 1162 1629
Size: 70 445 647 467
Fragments arranged by size:
647 467 445 70
MspA1I CmG'CkG
Cuts at: 0 721 914 1629
Size: 721 193 715
Fragments arranged by size:
721 715 193
NaeI GCC'GGC
Cuts at: 0 888 1105 1629
Size: 888 217 524
Fragments arranged by size:
888 524 217
NarI GG'CG_CC
Cuts at: 0 664 1629
Size: 664 965
NcoI C'CATG_G
Cuts at: 0 1184 1629
Size: 1184 445
NgoMIV G'CCGG_C
Cuts at: 0 886 1103 1629
Size: 886 217 526
Fragments arranged by size:
886 526 217
PfoI T'CCnGG_A
Cuts at: 0 167 1606 1629
Size: 167 1439 23
Fragments arranged by size:
1439 167 23
PspOMI G'GGCC_C
Cuts at: 0 767 1629
Size: 767 862
PspXI vC'TCGA_Gb
Cuts at: 0 569 1629
Size: 569 1060
PstI C_TGCA'G
Cuts at: 0 140 1629
Size: 140 1489
PvuII CAG'CTG
Cuts at: 0 721 1629
Size: 721 908
SacI G_AGCT'C
Cuts at: 0 428 1583 1629
Size: 428 1155 46
Fragments arranged by size:
1155 428 46
SfcI C'TryA_G
Cuts at: 0 136 1629
Size: 136 1493
SfoI GGC'GCC
Cuts at: 0 665 1629
Size: 665 964
SmlI C'TyrA_G
Cuts at: 0 569 1629
Size: 569 1060
StyI C'CwwG_G
Cuts at: 0 881 1184 1629
Size: 881 303 445
Fragments arranged by size:
881 445 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1064 1629
Size: 1064 565
TatI w'GTAC_w
Cuts at: 0 677 1629
Size: 677 952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 521 553 1629
Size: 521 32 1076
Fragments arranged by size:
1076 521 32
Tth111I GACn'n_nGTC
Cuts at: 0 280 1629
Size: 280 1349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1377 1629
Size: 1377 252
XhoI C'TCGA_G
Cuts at: 0 569 1629
Size: 569 1060
Enzymes that do cut:
AfeI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbvCI BclI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PfoI PspOMI PspXI PstI PvuII SacI
SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I
XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI AscI AseI AsiSI AvrII
BaeI BbsI BcgI BciVI BfrBI BglI BglII BlpI
BmtI BplI BpuEI BsaBI BsiWI BsmBI BspEI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XmaI
XmnI ZraI