(Linear) (Six-base) MAPSORT of: KIBB5384.seq  Check: 1510  from: 1  to: 618



KIBB5384.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     444     618 
   Size:        444     174
 
ApoI r'AATT_y

Cuts at:      0     228     247     618 
   Size:        228      19     371

  Fragments arranged by size: 

                 371     228      19
 
AvaI C'yCGr_G

Cuts at:      0     422     618 
   Size:        422     196
 
AvrII C'CTAG_G

Cuts at:      0     240     618 
   Size:        240     378
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      14     618 
   Size:         14     604
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     492     526     618 
   Size:        492      34      92

  Fragments arranged by size: 

                 492      92      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     618 
   Size:         52     566
 
BsaBI GATnn'nnATC

Cuts at:      0     382     618 
   Size:        382     236
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     618 
   Size:         49     569
 
BseYI C'CCAG_C

Cuts at:      0     606     618 
   Size:        606      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     444     618 
   Size:        444     174
 
BsrBI CCG'CTC

Cuts at:      0     461     618 
   Size:        461     157
 
BsrDI GCAATG_nn'

Cuts at:      0      30     510     618 
   Size:         30     480     108

  Fragments arranged by size: 

                 480     108      30
 
BssSI C'ACGA_G

Cuts at:      0     353     618 
   Size:        353     265
 
ClaI AT'CG_AT

Cuts at:      0     512     618 
   Size:        512     106
 
EaeI y'GGCC_r

Cuts at:      0       2     338     380     618 
   Size:          2     336      42     238

  Fragments arranged by size: 

                 336     238      42       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     618 
   Size:        104     514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     618 
   Size:         52     566
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     451     618 
   Size:        451     167
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     314     618 
   Size:        314     304
 
FspI TGC'GCA

Cuts at:      0      22     618 
   Size:         22     596
 
HindIII A'AGCT_T

Cuts at:      0     280     618 
   Size:        280     338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     584     618 
   Size:        128     456      34

  Fragments arranged by size: 

                 456     128      34
 
MscI TGG'CCA

Cuts at:      0     340     382     618 
   Size:        340      42     236

  Fragments arranged by size: 

                 340     236      42
 
NspI r_CATG'y

Cuts at:      0      21     618 
   Size:         21     597
 
StyI C'CwwG_G

Cuts at:      0     240     618 
   Size:        240     378
 

 Enzymes that do cut:

     AarI     ApoI     AvaI    AvrII     BbsI     BcgI     BpmI    BsaBI
    BseRI    BseYI    BspMI    BsrBI    BsrDI    BssSI     ClaI     EaeI
     EarI  Eco57MI    EcoNI     FalI     FspI  HindIII     MmeI     MscI
     NspI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FseI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI