Restriction map for
pF1KB5378
(Linear) (Six-base) MAPSORT of: KIBB5378.seq Check: 5863 from: 1 to: 2217
KIBB5378.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1029 2217
Size: 1029 1188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 482 668 842 1268 1826 2217
Size: 58 424 186 174 426 558 391
Fragments arranged by size:
558 426 424 391 186 174 58
AflIII A'CryG_T
Cuts at: 0 134 212 2217
Size: 134 78 2005
Fragments arranged by size:
2005 134 78
AleI CACnn'nnGTG
Cuts at: 0 1072 1198 1687 2217
Size: 1072 126 489 530
Fragments arranged by size:
1072 530 489 126
AlwNI CAG_nnn'CTG
Cuts at: 0 1266 2217
Size: 1266 951
ApaLI G'TGCA_C
Cuts at: 0 216 2217
Size: 216 2001
ApoI r'AATT_y
Cuts at: 0 554 1346 1951 2217
Size: 554 792 605 266
Fragments arranged by size:
792 605 554 266
BamHI G'GATC_C
Cuts at: 0 513 1638 2217
Size: 513 1125 579
Fragments arranged by size:
1125 579 513
BanI G'GyrC_C
Cuts at: 0 472 1689 2217
Size: 472 1217 528
Fragments arranged by size:
1217 528 472
BanII G_rGCy'C
Cuts at: 0 875 974 1851 2217
Size: 875 99 877 366
Fragments arranged by size:
877 875 366 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 1165 2217
Size: 1165 1052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1046 1080 2217
Size: 1046 34 1137
Fragments arranged by size:
1137 1046 34
BfrBI ATG'CAT
Cuts at: 0 441 2217
Size: 441 1776
BglI GCCn_nnn'nGGC
Cuts at: 0 1714 2217
Size: 1714 503
BglII A'GATC_T
Cuts at: 0 240 905 2120 2217
Size: 240 665 1215 97
Fragments arranged by size:
1215 665 240 97
BlpI GC'TnA_GC
Cuts at: 0 1906 2217
Size: 1906 311
Bme1580I G_kGCm'C
Cuts at: 0 220 477 2217
Size: 220 257 1740
Fragments arranged by size:
1740 257 220
BmgBI CAC'GTC
Cuts at: 0 24 2217
Size: 24 2193
BmrI ACTGGGnnnn_n'
Cuts at: 0 1722 2217
Size: 1722 495
BmtI G_CTAG'C
Cuts at: 0 847 2217
Size: 847 1370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1865 1954 2217
Size: 1865 89 263
Fragments arranged by size:
1865 263 89
Bpu10I CC'TnA_GC
Cuts at: 0 2054 2217
Size: 2054 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 558 715 2217
Size: 107 451 157 1502
Fragments arranged by size:
1502 451 157 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 548 1211 1549 2030 2217
Size: 548 663 338 481 187
Fragments arranged by size:
663 548 481 338 187
BsaAI yAC'GTr
Cuts at: 0 804 2217
Size: 804 1413
BsaWI w'CCGG_w
Cuts at: 0 86 2217
Size: 86 2131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 210 240 312 342 563 593 991
Size: 28 182 30 72 30 221 30 398
Cuts at: 991 1021 1817 1847 2217
Size: 30 796 30 370
Fragments arranged by size:
796 398 370 221 182 72 30 30
30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1063 1552 2217
Size: 1063 489 665
Fragments arranged by size:
1063 665 489
BseYI C'CCAG_C
Cuts at: 0 1167 1664 2020 2217
Size: 1167 497 356 197
Fragments arranged by size:
1167 497 356 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1050 1054 1225 2217
Size: 370 680 4 171 992
Fragments arranged by size:
992 680 370 171 4
BsiHKAI G_wGCw'C
Cuts at: 0 220 875 1187 2055 2217
Size: 220 655 312 868 162
Fragments arranged by size:
868 655 312 220 162
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2146 2217
Size: 2146 71
Bsp1286I G_dGCh'C
Cuts at: 0 220 477 875 974 1187 1851 2055 2217
Size: 220 257 398 99 213 664 204 162
Fragments arranged by size:
664 398 257 220 213 204 162 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1029 1470 2217
Size: 1029 441 747
Fragments arranged by size:
1029 747 441
BsrDI GCAATG_nn'
Cuts at: 0 593 1118 2015 2217
Size: 593 525 897 202
Fragments arranged by size:
897 593 525 202
BsrGI T'GTAC_A
Cuts at: 0 209 2217
Size: 209 2008
BstAPI GCAn_nnn'nTGC
Cuts at: 0 278 1041 1096 1935 2217
Size: 278 763 55 839 282
Fragments arranged by size:
839 763 282 278 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 1200 2217
Size: 297 903 1017
Fragments arranged by size:
1017 903 297
BstYI r'GATC_y
Cuts at: 0 240 513 905 1638 2120 2217
Size: 240 273 392 733 482 97
Fragments arranged by size:
733 482 392 273 240 97
Bsu36I CC'TnA_GG
Cuts at: 0 334 2217
Size: 334 1883
BtgI C'CryG_G
Cuts at: 0 1335 2032 2217
Size: 1335 697 185
Fragments arranged by size:
1335 697 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1989 2217
Size: 1989 228
BtsI GCAGTG_nn'
Cuts at: 0 690 1430 2217
Size: 690 740 787
Fragments arranged by size:
787 740 690
EaeI y'GGCC_r
Cuts at: 0 254 2029 2217
Size: 254 1775 188
Fragments arranged by size:
1775 254 188
EarI CTCTTCn'nnn_
Cuts at: 0 1274 1552 1802 1985 2217
Size: 1274 278 250 183 232
Fragments arranged by size:
1274 278 250 232 183
EcoICRI GAG'CTC
Cuts at: 0 873 2217
Size: 873 1344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 482 668 842 1268 1826 1865 1954
Size: 58 424 186 174 426 558 39 89
Cuts at: 1954 2217
Size: 263
Fragments arranged by size:
558 426 424 263 186 174 89 58
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 405 2217
Size: 405 1812
EcoO109I rG'GnC_Cy
Cuts at: 0 835 1263 1792 2217
Size: 835 428 529 425
Fragments arranged by size:
835 529 428 425
EcoRI G'AATT_C
Cuts at: 0 1346 1951 2217
Size: 1346 605 266
Fragments arranged by size:
1346 605 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 429 436 461 563 595 2217
Size: 404 25 7 25 102 32 1622
Fragments arranged by size:
1622 404 102 32 25 25 7
MfeI C'AATT_G
Cuts at: 0 518 2198 2217
Size: 518 1680 19
Fragments arranged by size:
1680 518 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1376 2217
Size: 698 678 841
Fragments arranged by size:
841 698 678
MscI TGG'CCA
Cuts at: 0 256 2217
Size: 256 1961
MslI CAynn'nnrTG
Cuts at: 0 1072 1198 1687 2008 2217
Size: 1072 126 489 321 209
Fragments arranged by size:
1072 489 321 209 126
MspA1I CmG'CkG
Cuts at: 0 1411 2217
Size: 1411 806
NcoI C'CATG_G
Cuts at: 0 1335 2032 2217
Size: 1335 697 185
Fragments arranged by size:
1335 697 185
NdeI CA'TA_TG
Cuts at: 0 222 2217
Size: 222 1995
NheI G'CTAG_C
Cuts at: 0 843 2217
Size: 843 1374
NsiI A_TGCA'T
Cuts at: 0 443 2217
Size: 443 1774
NspI r_CATG'y
Cuts at: 0 138 216 327 1482 2217
Size: 138 78 111 1155 735
Fragments arranged by size:
1155 735 138 111 78
PciI A'CATG_T
Cuts at: 0 134 212 2217
Size: 134 78 2005
Fragments arranged by size:
2005 134 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 1699 2217
Size: 1699 518
PpuMI rG'GwC_Cy
Cuts at: 0 835 1792 2217
Size: 835 957 425
Fragments arranged by size:
957 835 425
PshAI GACnn'nnGTC
Cuts at: 0 1541 2217
Size: 1541 676
PvuII CAG'CTG
Cuts at: 0 1411 2217
Size: 1411 806
SacI G_AGCT'C
Cuts at: 0 875 2217
Size: 875 1342
SapI GCTCTTCn'nnn_
Cuts at: 0 1985 2217
Size: 1985 232
SexAI A'CCwGG_T
Cuts at: 0 921 1220 2217
Size: 921 299 997
Fragments arranged by size:
997 921 299
SfcI C'TryA_G
Cuts at: 0 176 988 2217
Size: 176 812 1229
Fragments arranged by size:
1229 812 176
SmlI C'TyrA_G
Cuts at: 0 122 537 730 2217
Size: 122 415 193 1487
Fragments arranged by size:
1487 415 193 122
StyI C'CwwG_G
Cuts at: 0 9 423 1335 1939 2032 2217
Size: 9 414 912 604 93 185
Fragments arranged by size:
912 604 414 185 93 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1553 2217
Size: 1553 664
TatI w'GTAC_w
Cuts at: 0 209 1243 2217
Size: 209 1034 974
Fragments arranged by size:
1034 974 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 210 242 330 362 991 1023 2217
Size: 210 32 88 32 629 32 1194
Fragments arranged by size:
1194 629 210 88 32 32 32
XbaI T'CTAG_A
Cuts at: 0 546 2217
Size: 546 1671
XmnI GAAnn'nnTTC
Cuts at: 0 1346 1403 2217
Size: 1346 57 814
Fragments arranged by size:
1346 814 57
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaLI ApoI BamHI
BanI BanII BbsI BcgI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I MfeI MmeI MscI MslI MspA1I
NcoI NdeI NheI NsiI NspI PciI PflMI PpuMI
PshAI PvuII SacI SapI SexAI SfcI SmlI StyI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BciVI BclI BplI BsaBI BsaHI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI NaeI
NarI NgoMIV NotI NruI PacI PasI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XcmI XhoI XmaI ZraI