Restriction map for
pF1KB5377
(Linear) (Six-base) MAPSORT of: KIBB5377.seq Check: 6786 from: 1 to: 1020
KIBB5377.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 259 1020
Size: 259 761
AleI CACnn'nnGTG
Cuts at: 0 427 1020
Size: 427 593
AlwNI CAG_nnn'CTG
Cuts at: 0 84 533 1020
Size: 84 449 487
Fragments arranged by size:
487 449 84
AvaI C'yCGr_G
Cuts at: 0 922 1020
Size: 922 98
BanI G'GyrC_C
Cuts at: 0 118 250 259 1020
Size: 118 132 9 761
Fragments arranged by size:
761 132 118 9
BanII G_rGCy'C
Cuts at: 0 116 464 1020
Size: 116 348 556
Fragments arranged by size:
556 348 116
BbeI G_GCGC'C
Cuts at: 0 122 1020
Size: 122 898
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1020
Size: 605 415
BbvCI CC'TCA_GC
Cuts at: 0 146 1020
Size: 146 874
BglI GCCn_nnn'nGGC
Cuts at: 0 233 1020
Size: 233 787
BglII A'GATC_T
Cuts at: 0 185 521 1020
Size: 185 336 499
Fragments arranged by size:
499 336 185
BlpI GC'TnA_GC
Cuts at: 0 163 1020
Size: 163 857
BmgBI CAC'GTC
Cuts at: 0 159 1020
Size: 159 861
BmrI ACTGGGnnnn_n'
Cuts at: 0 224 481 1020
Size: 224 257 539
Fragments arranged by size:
539 257 224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 919 1020
Size: 919 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1020
Size: 914 106
Bpu10I CC'TnA_GC
Cuts at: 0 146 1020
Size: 146 874
BsaI GGTCTCn'nnnn_
Cuts at: 0 281 665 1020
Size: 281 384 355
Fragments arranged by size:
384 355 281
BsaBI GATnn'nnATC
Cuts at: 0 550 1020
Size: 550 470
BsaHI Gr'CG_yC
Cuts at: 0 119 1020
Size: 119 901
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 781 811 1020
Size: 664 30 87 30 209
Fragments arranged by size:
664 209 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 139 1020
Size: 67 72 881
Fragments arranged by size:
881 72 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 1020
Size: 789 231
BsiEI CG_ry'CG
Cuts at: 0 195 1020
Size: 195 825
BsiHKAI G_wGCw'C
Cuts at: 0 164 1020
Size: 164 856
BsmI GAATG_Cn'
Cuts at: 0 587 1020
Size: 587 433
Bsp1286I G_dGCh'C
Cuts at: 0 116 164 464 1020
Size: 116 48 300 556
Fragments arranged by size:
556 300 116 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 996 1020
Size: 996 24
BsrBI CCG'CTC
Cuts at: 0 443 449 1020
Size: 443 6 571
Fragments arranged by size:
571 443 6
BstBI TT'CG_AA
Cuts at: 0 591 1020
Size: 591 429
BstYI r'GATC_y
Cuts at: 0 185 480 521 1020
Size: 185 295 41 499
Fragments arranged by size:
499 295 185 41
BtgI C'CryG_G
Cuts at: 0 36 282 1020
Size: 36 246 738
Fragments arranged by size:
738 246 36
DraI TTT'AAA
Cuts at: 0 716 1020
Size: 716 304
DrdI GACnn_nn'nnGTC
Cuts at: 0 505 1020
Size: 505 515
EaeI y'GGCC_r
Cuts at: 0 438 1020
Size: 438 582
EarI CTCTTCn'nnn_
Cuts at: 0 512 1020
Size: 512 508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1020
Size: 914 106
EcoNI CCTnn'n_nnAGG
Cuts at: 0 787 896 1020
Size: 787 109 124
Fragments arranged by size:
787 124 109
EcoO109I rG'GnC_Cy
Cuts at: 0 936 1020
Size: 936 84
EcoRV GAT'ATC
Cuts at: 0 702 1020
Size: 702 318
HaeII r_GCGC'y
Cuts at: 0 85 122 1020
Size: 85 37 898
Fragments arranged by size:
898 85 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 664 696 1020
Size: 376 32 256 32 324
Fragments arranged by size:
376 324 256 32 32
KasI G'GCGC_C
Cuts at: 0 118 1020
Size: 118 902
KpnI G_GTAC'C
Cuts at: 0 263 1020
Size: 263 757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 707 1020
Size: 26 681 313
Fragments arranged by size:
681 313 26
MslI CAynn'nnrTG
Cuts at: 0 427 619 1020
Size: 427 192 401
Fragments arranged by size:
427 401 192
MspA1I CmG'CkG
Cuts at: 0 38 354 530 697 1020
Size: 38 316 176 167 323
Fragments arranged by size:
323 316 176 167 38
NarI GG'CG_CC
Cuts at: 0 119 1020
Size: 119 901
NcoI C'CATG_G
Cuts at: 0 282 1020
Size: 282 738
NspI r_CATG'y
Cuts at: 0 879 1020
Size: 879 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 24 394 1020
Size: 24 370 626
Fragments arranged by size:
626 370 24
PfoI T'CCnGG_A
Cuts at: 0 785 891 1020
Size: 785 106 129
Fragments arranged by size:
785 129 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 1020
Size: 115 32 873
Fragments arranged by size:
873 115 32
PpuMI rG'GwC_Cy
Cuts at: 0 936 1020
Size: 936 84
PvuII CAG'CTG
Cuts at: 0 697 1020
Size: 697 323
SacII CC_GC'GG
Cuts at: 0 39 1020
Size: 39 981
SfcI C'TryA_G
Cuts at: 0 525 1020
Size: 525 495
SfoI GGC'GCC
Cuts at: 0 120 1020
Size: 120 900
SmlI C'TyrA_G
Cuts at: 0 1000 1020
Size: 1000 20
SphI G_CATG'C
Cuts at: 0 879 1020
Size: 879 141
SspI AAT'ATT
Cuts at: 0 608 1020
Size: 608 412
StuI AGG'CCT
Cuts at: 0 151 212 870 902 1020
Size: 151 61 658 32 118
Fragments arranged by size:
658 151 118 61 32
StyI C'CwwG_G
Cuts at: 0 282 662 1020
Size: 282 380 358
Fragments arranged by size:
380 358 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 508 1020
Size: 403 105 512
Fragments arranged by size:
512 403 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 1020
Size: 781 32 207
Fragments arranged by size:
781 207 32
XmnI GAAnn'nnTTC
Cuts at: 0 974 1020
Size: 974 46
Enzymes that do cut:
Acc65I AleI AlwNI AvaI BanI BanII BbeI BbsI
BbvCI BglI BglII BlpI BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BstBI BstYI BtgI
DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KasI KpnI MmeI MslI MspA1I NarI
NcoI NspI PflMI PfoI PpiI PpuMI PvuII SacII
SfcI SfoI SmlI SphI SspI StuI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI Bme1580I BmtI BpuEI BsaAI BsaWI BseYI BsiWI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraIII EagI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI