Restriction map for
pF1KB5361
(Linear) (Six-base) MAPSORT of: KIBB5361.seq Check: 4952 from: 1 to: 1287
KIBB5361.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
Acc65I G'GTAC_C
Cuts at: 0 32 1287
Size: 32 1255
AhdI GACnn_n'nnGTC
Cuts at: 0 1268 1287
Size: 1268 19
AlwNI CAG_nnn'CTG
Cuts at: 0 547 1143 1287
Size: 547 596 144
Fragments arranged by size:
596 547 144
ApaLI G'TGCA_C
Cuts at: 0 178 1287
Size: 178 1109
AvaI C'yCGr_G
Cuts at: 0 901 1287
Size: 901 386
BamHI G'GATC_C
Cuts at: 0 817 1287
Size: 817 470
BanI G'GyrC_C
Cuts at: 0 32 1287
Size: 32 1255
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 1287
Size: 509 778
BglI GCCn_nnn'nGGC
Cuts at: 0 997 1287
Size: 997 290
BglII A'GATC_T
Cuts at: 0 633 1287
Size: 633 654
BlpI GC'TnA_GC
Cuts at: 0 965 1136 1287
Size: 965 171 151
Fragments arranged by size:
965 171 151
Bme1580I G_kGCm'C
Cuts at: 0 182 1287
Size: 182 1105
BmgBI CAC'GTC
Cuts at: 0 24 1287
Size: 24 1263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1287
Size: 82 1205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 827 1287
Size: 206 621 460
Fragments arranged by size:
621 460 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 1287
Size: 289 998
BsaBI GATnn'nnATC
Cuts at: 0 632 1287
Size: 632 655
BsaHI Gr'CG_yC
Cuts at: 0 826 1287
Size: 826 461
BsaWI w'CCGG_w
Cuts at: 0 744 1287
Size: 744 543
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 1287
Size: 79 1208
BseYI C'CCAG_C
Cuts at: 0 321 892 1070 1169 1180 1287
Size: 321 571 178 99 11 107
Fragments arranged by size:
571 321 178 107 99 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1287
Size: 104 1183
BsiHKAI G_wGCw'C
Cuts at: 0 182 1287
Size: 182 1105
BsmI GAATG_Cn'
Cuts at: 0 143 1287
Size: 143 1144
Bsp1286I G_dGCh'C
Cuts at: 0 182 1287
Size: 182 1105
BspEI T'CCGG_A
Cuts at: 0 744 1287
Size: 744 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 1287
Size: 62 1225
BsrBI CCG'CTC
Cuts at: 0 865 1287
Size: 865 422
BsrDI GCAATG_nn'
Cuts at: 0 590 854 1153 1287
Size: 590 264 299 134
Fragments arranged by size:
590 299 264 134
BsrFI r'CCGG_y
Cuts at: 0 712 1287
Size: 712 575
BstAPI GCAn_nnn'nTGC
Cuts at: 0 709 1287
Size: 709 578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1078 1287
Size: 1078 209
BstYI r'GATC_y
Cuts at: 0 633 817 1287
Size: 633 184 470
Fragments arranged by size:
633 470 184
BtgI C'CryG_G
Cuts at: 0 995 1287
Size: 995 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 1287
Size: 123 1164
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1287
Size: 9 1278
EaeI y'GGCC_r
Cuts at: 0 47 998 1287
Size: 47 951 289
Fragments arranged by size:
951 289 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1253 1287
Size: 1253 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1287
Size: 82 1205
EcoNI CCTnn'n_nnAGG
Cuts at: 0 622 1287
Size: 622 665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 304 1287
Size: 304 983
HaeII r_GCGC'y
Cuts at: 0 547 1287
Size: 547 740
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 861 893 1254 1286 1287
Size: 79 32 750 32 361 32 1
Fragments arranged by size:
750 361 79 32 32 32 1
HincII GTy'rAC
Cuts at: 0 204 1287
Size: 204 1083
HpaI GTT'AAC
Cuts at: 0 204 1287
Size: 204 1083
KpnI G_GTAC'C
Cuts at: 0 36 1287
Size: 36 1251
MscI TGG'CCA
Cuts at: 0 49 1000 1287
Size: 49 951 287
Fragments arranged by size:
951 287 49
MslI CAynn'nnrTG
Cuts at: 0 130 1000 1287
Size: 130 870 287
Fragments arranged by size:
870 287 130
MspA1I CmG'CkG
Cuts at: 0 325 1143 1287
Size: 325 818 144
Fragments arranged by size:
818 325 144
NaeI GCC'GGC
Cuts at: 0 714 1287
Size: 714 573
NcoI C'CATG_G
Cuts at: 0 995 1287
Size: 995 292
NgoMIV G'CCGG_C
Cuts at: 0 712 1287
Size: 712 575
NspI r_CATG'y
Cuts at: 0 243 1029 1287
Size: 243 786 258
Fragments arranged by size:
786 258 243
PflMI CCAn_nnn'nTGG
Cuts at: 0 1176 1287
Size: 1176 111
PshAI GACnn'nnGTC
Cuts at: 0 377 1287
Size: 377 910
PstI C_TGCA'G
Cuts at: 0 705 1011 1287
Size: 705 306 276
Fragments arranged by size:
705 306 276
PvuII CAG'CTG
Cuts at: 0 325 1143 1287
Size: 325 818 144
Fragments arranged by size:
818 325 144
SbfI CC_TGCA'GG
Cuts at: 0 1011 1287
Size: 1011 276
SfcI C'TryA_G
Cuts at: 0 701 1007 1287
Size: 701 306 280
Fragments arranged by size:
701 306 280
SmaI CCC'GGG
Cuts at: 0 903 1287
Size: 903 384
SmlI C'TyrA_G
Cuts at: 0 221 842 1287
Size: 221 621 445
Fragments arranged by size:
621 445 221
SphI G_CATG'C
Cuts at: 0 243 1029 1287
Size: 243 786 258
Fragments arranged by size:
786 258 243
StyI C'CwwG_G
Cuts at: 0 995 1287
Size: 995 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 389 1009 1033 1084 1287
Size: 389 620 24 51 203
Fragments arranged by size:
620 389 203 51 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 114 146 1287
Size: 114 32 1141
Fragments arranged by size:
1141 114 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 1117 1177 1287
Size: 57 1060 60 110
Fragments arranged by size:
1060 110 60 57
XmaI C'CCGG_G
Cuts at: 0 901 1287
Size: 901 386
XmnI GAAnn'nnTTC
Cuts at: 0 230 440 1287
Size: 230 210 847
Fragments arranged by size:
847 230 210
Enzymes that do cut:
Acc65I AhdI AlwNI ApaLI AvaI BamHI BanI BbsI
BglI BglII BlpI Bme1580I BmgBI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI BtgI BtgZI DrdI EaeI EciI Eco57MI EcoNI
FalI HaeII Hin4I HincII HpaI KpnI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PflMI PshAI PstI
PvuII SbfI SfcI SmaI SmlI SphI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BmrI BmtI BplI Bpu10I BsaAI BsaXI
BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
EagI EarI EcoICRI EcoO109I EcoRI EcoRV FseI FspI
FspAI HindIII KasI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI