(Linear) (Six-base) MAPSORT of: KIBB5353.seq  Check: 8916  from: 1  to: 1173



KIBB5353.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     932     968    1173 
   Size:        598     334      36     205

  Fragments arranged by size: 

                 598     334     205      36
 
AlwNI CAG_nnn'CTG

Cuts at:      0     616    1173 
   Size:        616     557
 
ApaLI G'TGCA_C

Cuts at:      0     263    1173 
   Size:        263     910
 
ApoI r'AATT_y

Cuts at:      0     280     497     505     643    1173 
   Size:        280     217       8     138     530

  Fragments arranged by size: 

                 530     280     217     138       8
 
AseI AT'TA_AT

Cuts at:      0     362    1173 
   Size:        362     811
 
AvrII C'CTAG_G

Cuts at:      0     211    1173 
   Size:        211     962
 
BanI G'GyrC_C

Cuts at:      0     770    1173 
   Size:        770     403
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     491    1173 
   Size:        491     682
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     187    1173 
   Size:        187     986
 
BclI T'GATC_A

Cuts at:      0      36    1173 
   Size:         36    1137
 
BglII A'GATC_T

Cuts at:      0     930    1173 
   Size:        930     243
 
Bme1580I G_kGCm'C

Cuts at:      0     267    1173 
   Size:        267     906
 
BmgBI CAC'GTC

Cuts at:      0     106     844    1173 
   Size:        106     738     329

  Fragments arranged by size: 

                 738     329     106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1049    1173 
   Size:       1049     124
 
BsaAI yAC'GTr

Cuts at:      0     991    1173 
   Size:        991     182
 
BsaBI GATnn'nnATC

Cuts at:      0     725    1173 
   Size:        725     448
 
BseYI C'CCAG_C

Cuts at:      0    1152    1173 
   Size:       1152      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     267    1173 
   Size:        267     906
 
BsmI GAATG_Cn'

Cuts at:      0     240    1173 
   Size:        240     933
 
Bsp1286I G_dGCh'C

Cuts at:      0     267    1173 
   Size:        267     906
 
BspHI T'CATG_A

Cuts at:      0     297    1173 
   Size:        297     876
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     257    1173 
   Size:        257     916
 
BsrGI T'GTAC_A

Cuts at:      0     763    1173 
   Size:        763     410
 
BstBI TT'CG_AA

Cuts at:      0     971    1173 
   Size:        971     202
 
BstYI r'GATC_y

Cuts at:      0     930    1173 
   Size:        930     243
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     825     860    1173 
   Size:        825      35     313

  Fragments arranged by size: 

                 825     313      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     170    1173 
   Size:        170    1003
 
EaeI y'GGCC_r

Cuts at:      0     618    1173 
   Size:        618     555
 
EarI CTCTTCn'nnn_

Cuts at:      0      65    1173 
   Size:         65    1108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     932     968    1049    1173 
   Size:        598     334      36      81     124

  Fragments arranged by size: 

                 598     334     124      81      36
 
EcoRI G'AATT_C

Cuts at:      0     497    1173 
   Size:        497     676
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     181     973    1173 
   Size:        181     792     200

  Fragments arranged by size: 

                 792     200     181
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     117     149     915     947    1173 
   Size:        117      32     766      32     226

  Fragments arranged by size: 

                 766     226     117      32      32
 
HincII GTy'rAC

Cuts at:      0     515     534    1173 
   Size:        515      19     639

  Fragments arranged by size: 

                 639     515      19
 
HpaI GTT'AAC

Cuts at:      0     515    1173 
   Size:        515     658
 
MscI TGG'CCA

Cuts at:      0     620    1173 
   Size:        620     553
 
PstI C_TGCA'G

Cuts at:      0     966    1173 
   Size:        966     207
 
SfcI C'TryA_G

Cuts at:      0     962    1173 
   Size:        962     211
 
SspI AAT'ATT

Cuts at:      0     667    1173 
   Size:        667     506
 
StyI C'CwwG_G

Cuts at:      0     211    1173 
   Size:        211     962
 
TatI w'GTAC_w

Cuts at:      0     763    1173 
   Size:        763     410
 
XmnI GAAnn'nnTTC

Cuts at:      0     572    1173 
   Size:        572     601
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI     AseI    AvrII     BanI     BbsI
    BciVI     BclI    BglII Bme1580I    BmgBI     BpmI    BsaAI    BsaBI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrGI    BstBI
    BstYI    CspCI     DrdI     EaeI     EarI  Eco57MI    EcoRI     FalI
    Hin4I   HincII     HpaI     MscI     PstI     SfcI     SspI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI    AsiSI
     AvaI     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BfrBI
     BglI     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI