(Linear) (Six-base) MAPSORT of: KIBB5352.seq  Check: 9336  from: 1  to: 1335



KIBB5352.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     152     170    1335 
   Size:        152      18    1165

  Fragments arranged by size: 

                1165     152      18
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     598     607    1335 
   Size:        235     363       9     728

  Fragments arranged by size: 

                 728     363     235       9
 
AflIII A'CryG_T

Cuts at:      0    1061    1335 
   Size:       1061     274
 
AhdI GACnn_n'nnGTC

Cuts at:      0     746    1031    1335 
   Size:        746     285     304

  Fragments arranged by size: 

                 746     304     285
 
ApoI r'AATT_y

Cuts at:      0      70     406     880    1116    1335 
   Size:         70     336     474     236     219

  Fragments arranged by size: 

                 474     336     236     219      70
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1032    1065    1335 
   Size:       1032      33     270

  Fragments arranged by size: 

                1032     270      33
 
BanII G_rGCy'C

Cuts at:      0     924    1335 
   Size:        924     411
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     566    1335 
   Size:        566     769
 
BbvCI CC'TCA_GC

Cuts at:      0     934    1335 
   Size:        934     401
 
BglII A'GATC_T

Cuts at:      0     825    1335 
   Size:        825     510
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1317    1335 
   Size:       1317      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1073    1335 
   Size:       1073     262
 
Bpu10I CC'TnA_GC

Cuts at:      0     934    1335 
   Size:        934     401
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1301    1335 
   Size:       1006     295      34

  Fragments arranged by size: 

                1006     295      34
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     574     656    1335 
   Size:        574      82     679

  Fragments arranged by size: 

                 679     574      82
 
BsaBI GATnn'nnATC

Cuts at:      0      47    1335 
   Size:         47    1288
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1015    1335 
   Size:       1015     320
 
Bsp1286I G_dGCh'C

Cuts at:      0     924    1335 
   Size:        924     411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     804    1280    1335 
   Size:        804     476      55

  Fragments arranged by size: 

                 804     476      55
 
BstBI TT'CG_AA

Cuts at:      0     801    1335 
   Size:        801     534
 
BstYI r'GATC_y

Cuts at:      0     288     825    1335 
   Size:        288     537     510

  Fragments arranged by size: 

                 537     510     288
 
BstZ17I GTA'TAC

Cuts at:      0     171    1335 
   Size:        171    1164
 
BtgI C'CryG_G

Cuts at:      0     554    1335 
   Size:        554     781
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      12    1335 
   Size:         12    1323
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     213     248    1335 
   Size:        213      35    1087

  Fragments arranged by size: 

                1087     213      35
 
EaeI y'GGCC_r

Cuts at:      0    1253    1335 
   Size:       1253      82
 
EarI CTCTTCn'nnn_

Cuts at:      0     626     709    1018    1335 
   Size:        626      83     309     317

  Fragments arranged by size: 

                 626     317     309      83
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     367    1335 
   Size:         19     348     968

  Fragments arranged by size: 

                 968     348      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     598     607    1073    1335 
   Size:        235     363       9     466     262

  Fragments arranged by size: 

                 466     363     262     235       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     732     764    1017    1049    1335 
   Size:        732      32     253      32     286

  Fragments arranged by size: 

                 732     286     253      32      32
 
HincII GTy'rAC

Cuts at:      0     317     903    1335 
   Size:        317     586     432

  Fragments arranged by size: 

                 586     432     317
 
HpaI GTT'AAC

Cuts at:      0     903    1335 
   Size:        903     432
 
MfeI C'AATT_G

Cuts at:      0     269    1335 
   Size:        269    1066
 
MscI TGG'CCA

Cuts at:      0    1255    1335 
   Size:       1255      80
 
MslI CAynn'nnrTG

Cuts at:      0    1060    1335 
   Size:       1060     275
 
NcoI C'CATG_G

Cuts at:      0     554    1335 
   Size:        554     781
 
NruI TCG'CGA

Cuts at:      0     686    1335 
   Size:        686     649
 
NspI r_CATG'y

Cuts at:      0    1065    1335 
   Size:       1065     270
 
PciI A'CATG_T

Cuts at:      0    1061    1335 
   Size:       1061     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1251    1335 
   Size:       1251      84
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     455     487    1335 
   Size:        455      32     848

  Fragments arranged by size: 

                 848     455      32
 
SfcI C'TryA_G

Cuts at:      0     393     773    1335 
   Size:        393     380     562

  Fragments arranged by size: 

                 562     393     380
 
SmlI C'TyrA_G

Cuts at:      0     985    1316    1335 
   Size:        985     331      19

  Fragments arranged by size: 

                 985     331      19
 
SspI AAT'ATT

Cuts at:      0     379     693    1263    1335 
   Size:        379     314     570      72

  Fragments arranged by size: 

                 570     379     314      72
 
StyI C'CwwG_G

Cuts at:      0     554    1335 
   Size:        554     781
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     418    1335 
   Size:        418     917
 
XmnI GAAnn'nnTTC

Cuts at:      0     964    1335 
   Size:        964     371
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     ApoI     BaeI    BanII     BbsI
    BbvCI    BglII     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsmBI Bsp1286I    BspMI    BstBI    BstYI  BstZ17I     BtgI    BtgZI
    CspCI     EaeI     EarI     EciI  Eco57MI    Hin4I   HincII     HpaI
     MfeI     MscI     MslI     NcoI     NruI     NspI     PciI    PflMI
     PpiI     SfcI     SmlI     SspI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI   Bsu36I     BtsI     ClaI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NsiI     PacI     PasI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI