Restriction map for
pF1KB5332
(Linear) (Six-base) MAPSORT of: KIBB5332.seq Check: 4554 from: 1 to: 825
KIBB5332.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AflIII A'CryG_T
Cuts at: 0 265 339 825
Size: 265 74 486
Fragments arranged by size:
486 265 74
AlwNI CAG_nnn'CTG
Cuts at: 0 474 825
Size: 474 351
ApoI r'AATT_y
Cuts at: 0 184 825
Size: 184 641
AseI AT'TA_AT
Cuts at: 0 208 825
Size: 208 617
AvaI C'yCGr_G
Cuts at: 0 254 283 350 374 598 825
Size: 254 29 67 24 224 227
Fragments arranged by size:
254 227 224 67 29 24
BanII G_rGCy'C
Cuts at: 0 41 825
Size: 41 784
BbsI GAAGACnn'nnnn_
Cuts at: 0 102 332 825
Size: 102 230 493
Fragments arranged by size:
493 230 102
BbvCI CC'TCA_GC
Cuts at: 0 119 825
Size: 119 706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 269 825
Size: 235 34 556
Fragments arranged by size:
556 235 34
BmgBI CAC'GTC
Cuts at: 0 347 825
Size: 347 478
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 825
Size: 153 672
Bpu10I CC'TnA_GC
Cuts at: 0 119 825
Size: 119 706
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 825
Size: 347 478
BsaAI yAC'GTr
Cuts at: 0 426 825
Size: 426 399
BsaWI w'CCGG_w
Cuts at: 0 384 825
Size: 384 441
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 825
Size: 346 30 449
Fragments arranged by size:
449 346 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 825
Size: 286 539
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 825
Size: 246 579
BsiHKAI G_wGCw'C
Cuts at: 0 41 292 825
Size: 41 251 533
Fragments arranged by size:
533 251 41
BsiWI C'GTAC_G
Cuts at: 0 5 825
Size: 5 820
BsmBI CGTCTCn'nnnn_
Cuts at: 0 353 825
Size: 353 472
Bsp1286I G_dGCh'C
Cuts at: 0 41 292 825
Size: 41 251 533
Fragments arranged by size:
533 251 41
BspHI T'CATG_A
Cuts at: 0 177 825
Size: 177 648
BsrDI GCAATG_nn'
Cuts at: 0 188 327 825
Size: 188 139 498
Fragments arranged by size:
498 188 139
BssSI C'ACGA_G
Cuts at: 0 793 825
Size: 793 32
BstYI r'GATC_y
Cuts at: 0 797 825
Size: 797 28
Bsu36I CC'TnA_GG
Cuts at: 0 574 825
Size: 574 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 99 825
Size: 99 726
DraIII CAC_nnn'GTG
Cuts at: 0 585 825
Size: 585 240
DrdI GACnn_nn'nnGTC
Cuts at: 0 52 825
Size: 52 773
EaeI y'GGCC_r
Cuts at: 0 89 607 825
Size: 89 518 218
Fragments arranged by size:
518 218 89
EcoICRI GAG'CTC
Cuts at: 0 39 825
Size: 39 786
EcoNI CCTnn'n_nnAGG
Cuts at: 0 536 825
Size: 536 289
EcoRI G'AATT_C
Cuts at: 0 184 825
Size: 184 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 344 346 376 378 628 660 825
Size: 344 2 30 2 250 32 165
Fragments arranged by size:
344 250 165 32 30 2 2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 825
Size: 389 436
MscI TGG'CCA
Cuts at: 0 91 609 825
Size: 91 518 216
Fragments arranged by size:
518 216 91
MslI CAynn'nnrTG
Cuts at: 0 385 825
Size: 385 440
NspI r_CATG'y
Cuts at: 0 269 343 520 825
Size: 269 74 177 305
Fragments arranged by size:
305 269 177 74
PciI A'CATG_T
Cuts at: 0 265 339 825
Size: 265 74 486
Fragments arranged by size:
486 265 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 143 825
Size: 143 682
PfoI T'CCnGG_A
Cuts at: 0 394 825
Size: 394 431
PstI C_TGCA'G
Cuts at: 0 248 540 825
Size: 248 292 285
Fragments arranged by size:
292 285 248
SacI G_AGCT'C
Cuts at: 0 41 825
Size: 41 784
ScaI AGT'ACT
Cuts at: 0 250 622 825
Size: 250 372 203
Fragments arranged by size:
372 250 203
SfcI C'TryA_G
Cuts at: 0 244 536 825
Size: 244 292 289
Fragments arranged by size:
292 289 244
SmaI CCC'GGG
Cuts at: 0 376 825
Size: 376 449
SmlI C'TyrA_G
Cuts at: 0 350 825
Size: 350 475
SphI G_CATG'C
Cuts at: 0 520 825
Size: 520 305
TatI w'GTAC_w
Cuts at: 0 248 620 825
Size: 248 372 205
Fragments arranged by size:
372 248 205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 825
Size: 423 402
XhoI C'TCGA_G
Cuts at: 0 350 825
Size: 350 475
XmaI C'CCGG_G
Cuts at: 0 374 825
Size: 374 451
Enzymes that do cut:
AflIII AlwNI ApoI AseI AvaI BanII BbsI BbvCI
BcgI BmgBI BmrI Bpu10I BsaI BsaAI BsaWI BsaXI
BseRI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BsrDI
BssSI BstYI Bsu36I BtgZI DraIII DrdI EaeI EcoICRI
EcoNI EcoRI Hin4I MmeI MscI MslI NspI PciI
PflMI PfoI PstI SacI ScaI SfcI SmaI SmlI
SphI TatI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BanI BbeI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpmI
BpuEI BsaBI BsaHI BseYI BsiEI BsmI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgI BtsI ClaI CspCI DraI EagI EarI
EciI Eco57MI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XmnI ZraI