Restriction map for
pF1KB5331
(Linear) (Six-base) MAPSORT of: KIBB5331.seq Check: 4878 from: 1 to: 1245
KIBB5331.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 1070 1245
Size: 1070 175
AccI GT'mk_AC
Cuts at: 0 1085 1245
Size: 1085 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1245
Size: 814 431
AflIII A'CryG_T
Cuts at: 0 566 707 1245
Size: 566 141 538
Fragments arranged by size:
566 538 141
AgeI A'CCGG_T
Cuts at: 0 211 1245
Size: 211 1034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 98 958 1245
Size: 98 860 287
Fragments arranged by size:
860 287 98
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1245
Size: 976 32 237
Fragments arranged by size:
976 237 32
AlwNI CAG_nnn'CTG
Cuts at: 0 93 1245
Size: 93 1152
ApaI G_GGCC'C
Cuts at: 0 123 1214 1245
Size: 123 1091 31
Fragments arranged by size:
1091 123 31
AvrII C'CTAG_G
Cuts at: 0 837 1245
Size: 837 408
BanII G_rGCy'C
Cuts at: 0 123 596 848 1214 1245
Size: 123 473 252 366 31
Fragments arranged by size:
473 366 252 123 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 1062 1169 1245
Size: 1062 107 76
Fragments arranged by size:
1062 107 76
BciVI GTATCCnnnnn_n'
Cuts at: 0 1185 1245
Size: 1185 60
BlpI GC'TnA_GC
Cuts at: 0 479 1245
Size: 479 766
Bme1580I G_kGCm'C
Cuts at: 0 123 1214 1245
Size: 123 1091 31
Fragments arranged by size:
1091 123 31
BmgBI CAC'GTC
Cuts at: 0 567 765 978 1245
Size: 567 198 213 267
Fragments arranged by size:
567 267 213 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 1245
Size: 117 1128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 967 1245
Size: 487 480 278
Fragments arranged by size:
487 480 278
Bpu10I CC'TnA_GC
Cuts at: 0 415 1245
Size: 415 830
BsaAI yAC'GTr
Cuts at: 0 710 1245
Size: 710 535
BsaBI GATnn'nnATC
Cuts at: 0 100 1245
Size: 100 1145
BsaHI Gr'CG_yC
Cuts at: 0 1067 1245
Size: 1067 178
BsaWI w'CCGG_w
Cuts at: 0 211 1245
Size: 211 1034
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 1245
Size: 556 30 659
Fragments arranged by size:
659 556 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 128 475 1141 1159 1162 1165 1168 1171
Size: 128 347 666 18 3 3 3 3
Cuts at: 1171 1245
Size: 74
Fragments arranged by size:
666 347 128 74 18 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 168 596 1174 1207 1245
Size: 168 428 578 33 38
Fragments arranged by size:
578 428 168 38 33
BsiHKAI G_wGCw'C
Cuts at: 0 865 1245
Size: 865 380
BsmI GAATG_Cn'
Cuts at: 0 662 1245
Size: 662 583
Bsp1286I G_dGCh'C
Cuts at: 0 123 596 848 865 1214 1245
Size: 123 473 252 17 349 31
Fragments arranged by size:
473 349 252 123 31 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1245
Size: 215 1030
BsrBI CCG'CTC
Cuts at: 0 471 1245
Size: 471 774
BsrDI GCAATG_nn'
Cuts at: 0 441 830 1245
Size: 441 389 415
Fragments arranged by size:
441 415 389
BsrFI r'CCGG_y
Cuts at: 0 211 368 865 1245
Size: 211 157 497 380
Fragments arranged by size:
497 380 211 157
BssHII G'CGCG_C
Cuts at: 0 910 912 1245
Size: 910 2 333
Fragments arranged by size:
910 333 2
BssSI C'ACGA_G
Cuts at: 0 1009 1196 1245
Size: 1009 187 49
Fragments arranged by size:
1009 187 49
BstEII G'GTnAC_C
Cuts at: 0 66 288 1245
Size: 66 222 957
Fragments arranged by size:
957 222 66
BstYI r'GATC_y
Cuts at: 0 699 1245
Size: 699 546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 215 1245
Size: 215 1030
BtsI GCAGTG_nn'
Cuts at: 0 1055 1245
Size: 1055 190
EarI CTCTTCn'nnn_
Cuts at: 0 23 1245
Size: 23 1222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 1245
Size: 331 914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 814 967 1245
Size: 487 327 153 278
Fragments arranged by size:
487 327 278 153
EcoNI CCTnn'n_nnAGG
Cuts at: 0 76 1120 1245
Size: 76 1044 125
Fragments arranged by size:
1044 125 76
EcoO109I rG'GnC_Cy
Cuts at: 0 119 1211 1245
Size: 119 1092 34
Fragments arranged by size:
1092 119 34
FspI TGC'GCA
Cuts at: 0 934 1245
Size: 934 311
HaeII r_GCGC'y
Cuts at: 0 1008 1245
Size: 1008 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 766 798 1245
Size: 556 32 178 32 447
Fragments arranged by size:
556 447 178 32 32
MspA1I CmG'CkG
Cuts at: 0 196 478 604 827 999 1174 1245
Size: 196 282 126 223 172 175 71
Fragments arranged by size:
282 223 196 175 172 126 71
NspI r_CATG'y
Cuts at: 0 336 1245
Size: 336 909
PfoI T'CCnGG_A
Cuts at: 0 728 980 1245
Size: 728 252 265
Fragments arranged by size:
728 265 252
PspOMI G'GGCC_C
Cuts at: 0 119 1210 1245
Size: 119 1091 35
Fragments arranged by size:
1091 119 35
PstI C_TGCA'G
Cuts at: 0 77 314 1004 1245
Size: 77 237 690 241
Fragments arranged by size:
690 241 237 77
PvuII CAG'CTG
Cuts at: 0 196 604 827 999 1174 1245
Size: 196 408 223 172 175 71
Fragments arranged by size:
408 223 196 175 172 71
RsrII CG'GwC_CG
Cuts at: 0 473 1245
Size: 473 772
ScaI AGT'ACT
Cuts at: 0 127 1030 1245
Size: 127 903 215
Fragments arranged by size:
903 215 127
SfcI C'TryA_G
Cuts at: 0 73 310 1000 1245
Size: 73 237 690 245
Fragments arranged by size:
690 245 237 73
SgrAI Cr'CCGG_yG
Cuts at: 0 211 1245
Size: 211 1034
StyI C'CwwG_G
Cuts at: 0 176 837 1214 1245
Size: 176 661 377 31
Fragments arranged by size:
661 377 176 31
TatI w'GTAC_w
Cuts at: 0 125 1028 1245
Size: 125 903 217
Fragments arranged by size:
903 217 125
ZraI GAC'GTC
Cuts at: 0 1068 1245
Size: 1068 177
Enzymes that do cut:
AatII AccI AcuI AflIII AgeI AlfI AloI AlwNI
ApaI AvrII BanII BbsI BciVI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstEII BstYI BtgZI BtsI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MspA1I
NspI PfoI PspOMI PstI PvuII RsrII ScaI SfcI
SgrAI StyI TatI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AhdI AleI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BmtI
BplI BpuEI BsaI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrGI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI