Restriction map for
pF1KB5326
(Linear) (Six-base) MAPSORT of: KIBB5326.seq Check: 2577 from: 1 to: 1350
KIBB5326.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 134 1350
Size: 134 1216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1350
Size: 613 737
AfeI AGC'GCT
Cuts at: 0 617 1350
Size: 617 733
AflII C'TTAA_G
Cuts at: 0 667 1350
Size: 667 683
AleI CACnn'nnGTG
Cuts at: 0 677 1350
Size: 677 673
AlwNI CAG_nnn'CTG
Cuts at: 0 73 1350
Size: 73 1277
ApoI r'AATT_y
Cuts at: 0 325 403 1192 1307 1350
Size: 325 78 789 115 43
Fragments arranged by size:
789 325 115 78 43
AvaI C'yCGr_G
Cuts at: 0 22 1350
Size: 22 1328
BamHI G'GATC_C
Cuts at: 0 438 1350
Size: 438 912
BanI G'GyrC_C
Cuts at: 0 9 42 1350
Size: 9 33 1308
Fragments arranged by size:
1308 33 9
BanII G_rGCy'C
Cuts at: 0 64 127 1350
Size: 64 63 1223
Fragments arranged by size:
1223 64 63
BbeI G_GCGC'C
Cuts at: 0 46 1350
Size: 46 1304
BfrBI ATG'CAT
Cuts at: 0 859 1350
Size: 859 491
BmrI ACTGGGnnnn_n'
Cuts at: 0 590 1049 1350
Size: 590 459 301
Fragments arranged by size:
590 459 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 588 1085 1350
Size: 19 569 497 265
Fragments arranged by size:
569 497 265 19
Bpu10I CC'TnA_GC
Cuts at: 0 995 1328 1350
Size: 995 333 22
Fragments arranged by size:
995 333 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 1350
Size: 507 843
BsaBI GATnn'nnATC
Cuts at: 0 175 1350
Size: 175 1175
BsaHI Gr'CG_yC
Cuts at: 0 43 1350
Size: 43 1307
BsaWI w'CCGG_w
Cuts at: 0 15 1350
Size: 15 1335
BseYI C'CCAG_C
Cuts at: 0 57 73 78 1350
Size: 57 16 5 1272
Fragments arranged by size:
1272 57 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1350
Size: 972 378
BsiEI CG_ry'CG
Cuts at: 0 92 1350
Size: 92 1258
BsiHKAI G_wGCw'C
Cuts at: 0 127 1350
Size: 127 1223
BsmI GAATG_Cn'
Cuts at: 0 1339 1350
Size: 1339 11
Bsp1286I G_dGCh'C
Cuts at: 0 64 127 1350
Size: 64 63 1223
Fragments arranged by size:
1223 64 63
BspEI T'CCGG_A
Cuts at: 0 15 1350
Size: 15 1335
BsrBI CCG'CTC
Cuts at: 0 27 1350
Size: 27 1323
BsrDI GCAATG_nn'
Cuts at: 0 937 1350
Size: 937 413
BsrFI r'CCGG_y
Cuts at: 0 39 1350
Size: 39 1311
BstYI r'GATC_y
Cuts at: 0 438 1350
Size: 438 912
BtsI GCAGTG_nn'
Cuts at: 0 1027 1350
Size: 1027 323
EarI CTCTTCn'nnn_
Cuts at: 0 113 1350
Size: 113 1237
EcoICRI GAG'CTC
Cuts at: 0 125 1350
Size: 125 1225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 588 613 1085 1350
Size: 19 569 25 472 265
Fragments arranged by size:
569 472 265 25 19
EcoO109I rG'GnC_Cy
Cuts at: 0 1260 1350
Size: 1260 90
EcoRI G'AATT_C
Cuts at: 0 1307 1350
Size: 1307 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 937 1350
Size: 937 413
HaeII r_GCGC'y
Cuts at: 0 46 619 1350
Size: 46 573 731
Fragments arranged by size:
731 573 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 822 854 978 991 1010 1023
Size: 509 32 281 32 124 13 19 13
Cuts at: 1023 1350
Size: 327
Fragments arranged by size:
509 327 281 124 32 32 19 13
13
HincII GTy'rAC
Cuts at: 0 135 1350
Size: 135 1215
KasI G'GCGC_C
Cuts at: 0 42 1350
Size: 42 1308
MslI CAynn'nnrTG
Cuts at: 0 223 278 677 848 1350
Size: 223 55 399 171 502
Fragments arranged by size:
502 399 223 171 55
MspA1I CmG'CkG
Cuts at: 0 57 591 1350
Size: 57 534 759
Fragments arranged by size:
759 534 57
NaeI GCC'GGC
Cuts at: 0 41 1350
Size: 41 1309
NarI GG'CG_CC
Cuts at: 0 43 1350
Size: 43 1307
NdeI CA'TA_TG
Cuts at: 0 845 1350
Size: 845 505
NgoMIV G'CCGG_C
Cuts at: 0 39 1350
Size: 39 1311
NsiI A_TGCA'T
Cuts at: 0 861 1350
Size: 861 489
NspI r_CATG'y
Cuts at: 0 228 1350
Size: 228 1122
PflMI CCAn_nnn'nTGG
Cuts at: 0 73 1350
Size: 73 1277
PstI C_TGCA'G
Cuts at: 0 692 830 1350
Size: 692 138 520
Fragments arranged by size:
692 520 138
PvuII CAG'CTG
Cuts at: 0 591 1350
Size: 591 759
SacI G_AGCT'C
Cuts at: 0 127 1350
Size: 127 1223
SalI G'TCGA_C
Cuts at: 0 133 1350
Size: 133 1217
SfcI C'TryA_G
Cuts at: 0 675 688 826 1350
Size: 675 13 138 524
Fragments arranged by size:
675 524 138 13
SfoI GGC'GCC
Cuts at: 0 44 1350
Size: 44 1306
SmlI C'TyrA_G
Cuts at: 0 486 667 1350
Size: 486 181 683
Fragments arranged by size:
683 486 181
StyI C'CwwG_G
Cuts at: 0 47 1350
Size: 47 1303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1350
Size: 805 545
TatI w'GTAC_w
Cuts at: 0 759 1350
Size: 759 591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1350
Size: 491 32 827
Fragments arranged by size:
827 491 32
XmnI GAAnn'nnTTC
Cuts at: 0 479 1350
Size: 479 871
Enzymes that do cut:
AccI AcuI AfeI AflII AleI AlwNI ApoI AvaI
BamHI BanI BanII BbeI BfrBI BmrI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BstYI BtsI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PflMI PstI PvuII SacI SalI SfcI
SfoI SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaXI BseRI
BsiWI BsmBI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI