(Linear) (Six-base) MAPSORT of: KIBB5319.seq  Check: 5955  from: 1  to: 1278



KIBB5319.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AccI GT'mk_AC

Cuts at:      0     707    1278 
   Size:        707     571
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     274    1278 
   Size:        142     132    1004

  Fragments arranged by size: 

                1004     142     132
 
AleI CACnn'nnGTG

Cuts at:      0     760    1278 
   Size:        760     518
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     961    1278 
   Size:        961     317
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     866     898    1278 
   Size:        866      32     380

  Fragments arranged by size: 

                 866     380      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1221    1278 
   Size:       1221      57
 
ApoI r'AATT_y

Cuts at:      0     840    1025    1042    1278 
   Size:        840     185      17     236

  Fragments arranged by size: 

                 840     236     185      17
 
BanI G'GyrC_C

Cuts at:      0     136    1278 
   Size:        136    1142
 
BanII G_rGCy'C

Cuts at:      0     719    1278 
   Size:        719     559
 
BbvCI CC'TCA_GC

Cuts at:      0     443    1278 
   Size:        443     835
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1198    1278 
   Size:       1198      80
 
Bpu10I CC'TnA_GC

Cuts at:      0     443    1278 
   Size:        443     835
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183    1278 
   Size:        183    1095
 
BsaAI yAC'GTr

Cuts at:      0    1120    1278 
   Size:       1120     158
 
BsaWI w'CCGG_w

Cuts at:      0     236     836    1278 
   Size:        236     600     442

  Fragments arranged by size: 

                 600     442     236
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     893     923    1278 
   Size:        893      30     355

  Fragments arranged by size: 

                 893     355      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     741    1278 
   Size:        741     537
 
BseYI C'CCAG_C

Cuts at:      0    1139    1278 
   Size:       1139     139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1186    1278 
   Size:       1186      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     700     719    1278 
   Size:        700      19     559

  Fragments arranged by size: 

                 700     559      19
 
BsmI GAATG_Cn'

Cuts at:      0     325     925    1278 
   Size:        325     600     353

  Fragments arranged by size: 

                 600     353     325
 
Bsp1286I G_dGCh'C

Cuts at:      0     700     719    1278 
   Size:        700      19     559

  Fragments arranged by size: 

                 700     559      19
 
BsrBI CCG'CTC

Cuts at:      0     206    1278 
   Size:        206    1072
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1181    1278 
   Size:       1181      97
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     692    1278 
   Size:        692     586
 
BstYI r'GATC_y

Cuts at:      0    1001    1278 
   Size:       1001     277
 
BtgI C'CryG_G

Cuts at:      0     140    1074    1278 
   Size:        140     934     204

  Fragments arranged by size: 

                 934     204     140
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      83    1278 
   Size:         83    1195
 
EarI CTCTTCn'nnn_

Cuts at:      0      37    1278 
   Size:         37    1241
 
EcoICRI GAG'CTC

Cuts at:      0     717    1278 
   Size:        717     561
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     274    1198    1278 
   Size:        142     132     924      80

  Fragments arranged by size: 

                 924     142     132      80
 
EcoRI G'AATT_C

Cuts at:      0     840    1278 
   Size:        840     438
 
HaeII r_GCGC'y

Cuts at:      0     279    1278 
   Size:        279     999
 
HincII GTy'rAC

Cuts at:      0     834    1278 
   Size:        834     444
 
HpaI GTT'AAC

Cuts at:      0     834    1278 
   Size:        834     444
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     815     879     934    1278 
   Size:        815      64      55     344

  Fragments arranged by size: 

                 815     344      64      55
 
MslI CAynn'nnrTG

Cuts at:      0     318     760     943    1278 
   Size:        318     442     183     335

  Fragments arranged by size: 

                 442     335     318     183
 
MspA1I CmG'CkG

Cuts at:      0      37    1278 
   Size:         37    1241
 
NcoI C'CATG_G

Cuts at:      0     140    1074    1278 
   Size:        140     934     204

  Fragments arranged by size: 

                 934     204     140
 
NspI r_CATG'y

Cuts at:      0     498    1278 
   Size:        498     780
 
PasI CC'CwG_GG

Cuts at:      0     172    1278 
   Size:        172    1106
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1221    1278 
   Size:       1221      57
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     949     981    1278 
   Size:        949      32     297

  Fragments arranged by size: 

                 949     297      32
 
PvuII CAG'CTG

Cuts at:      0      37    1278 
   Size:         37    1241
 
SacI G_AGCT'C

Cuts at:      0     719    1278 
   Size:        719     559
 
SmlI C'TyrA_G

Cuts at:      0     162    1278 
   Size:        162    1116
 
SnaBI TAC'GTA

Cuts at:      0    1120    1278 
   Size:       1120     158
 
StuI AGG'CCT

Cuts at:      0     160    1278 
   Size:        160    1118
 
StyI C'CwwG_G

Cuts at:      0     140     813    1074    1278 
   Size:        140     673     261     204

  Fragments arranged by size: 

                 673     261     204     140
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      44     721    1001    1278 
   Size:         44     677     280     277

  Fragments arranged by size: 

                 677     280     277      44
 
TatI w'GTAC_w

Cuts at:      0     399    1111    1278 
   Size:        399     712     167

  Fragments arranged by size: 

                 712     399     167
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     893     925    1278 
   Size:        893      32     353

  Fragments arranged by size: 

                 893     353      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     147     762    1278 
   Size:        147     615     516

  Fragments arranged by size: 

                 615     516     147
 
XmnI GAAnn'nnTTC

Cuts at:      0     805    1278 
   Size:        805     473
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI     AloI    AlwNI     ApoI     BanI
    BanII    BbvCI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     EarI  EcoICRI  Eco57MI    EcoRI
    HaeII   HincII     HpaI     MmeI     MslI   MspA1I     NcoI     NspI
     PasI    PflMI     PsrI    PvuII     SacI     SmlI    SnaBI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI