Restriction map for
pF1KB5302
(Linear) (Six-base) MAPSORT of: KIBB5302.seq Check: 3468 from: 1 to: 927
KIBB5302.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 495 927
Size: 495 432
AflII C'TTAA_G
Cuts at: 0 307 927
Size: 307 620
AflIII A'CryG_T
Cuts at: 0 894 927
Size: 894 33
AleI CACnn'nnGTG
Cuts at: 0 706 927
Size: 706 221
AlwNI CAG_nnn'CTG
Cuts at: 0 157 927
Size: 157 770
AvaI C'yCGr_G
Cuts at: 0 638 927
Size: 638 289
BanI G'GyrC_C
Cuts at: 0 749 927
Size: 749 178
BanII G_rGCy'C
Cuts at: 0 720 927
Size: 720 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 158 927
Size: 124 34 769
Fragments arranged by size:
769 124 34
BglII A'GATC_T
Cuts at: 0 441 485 927
Size: 441 44 442
Fragments arranged by size:
442 441 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 511 927
Size: 511 416
BsaWI w'CCGG_w
Cuts at: 0 758 927
Size: 758 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 242 272 927
Size: 242 30 655
Fragments arranged by size:
655 242 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 626 656 927
Size: 626 30 271
Fragments arranged by size:
626 271 30
BseYI C'CCAG_C
Cuts at: 0 870 927
Size: 870 57
BsiHKAI G_wGCw'C
Cuts at: 0 546 720 786 927
Size: 546 174 66 141
Fragments arranged by size:
546 174 141 66
Bsp1286I G_dGCh'C
Cuts at: 0 546 720 786 927
Size: 546 174 66 141
Fragments arranged by size:
546 174 141 66
BspHI T'CATG_A
Cuts at: 0 186 351 927
Size: 186 165 576
Fragments arranged by size:
576 186 165
BstEII G'GTnAC_C
Cuts at: 0 808 927
Size: 808 119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 927
Size: 27 900
BstYI r'GATC_y
Cuts at: 0 441 485 603 927
Size: 441 44 118 324
Fragments arranged by size:
441 324 118 44
BtgI C'CryG_G
Cuts at: 0 615 927
Size: 615 312
BtsI GCAGTG_nn'
Cuts at: 0 292 464 927
Size: 292 172 463
Fragments arranged by size:
463 292 172
EcoICRI GAG'CTC
Cuts at: 0 718 927
Size: 718 209
EcoNI CCTnn'n_nnAGG
Cuts at: 0 881 927
Size: 881 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 669 701 927
Size: 477 32 160 32 226
Fragments arranged by size:
477 226 160 32 32
HincII GTy'rAC
Cuts at: 0 261 927
Size: 261 666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 927
Size: 152 775
MslI CAynn'nnrTG
Cuts at: 0 692 706 927
Size: 692 14 221
Fragments arranged by size:
692 221 14
MspA1I CmG'CkG
Cuts at: 0 48 66 927
Size: 48 18 861
Fragments arranged by size:
861 48 18
NspI r_CATG'y
Cuts at: 0 898 927
Size: 898 29
PciI A'CATG_T
Cuts at: 0 894 927
Size: 894 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 927
Size: 30 897
PspXI vC'TCGA_Gb
Cuts at: 0 638 927
Size: 638 289
PvuII CAG'CTG
Cuts at: 0 48 66 927
Size: 48 18 861
Fragments arranged by size:
861 48 18
SacI G_AGCT'C
Cuts at: 0 720 927
Size: 720 207
SfcI C'TryA_G
Cuts at: 0 518 927
Size: 518 409
SmlI C'TyrA_G
Cuts at: 0 307 638 927
Size: 307 331 289
Fragments arranged by size:
331 307 289
SspI AAT'ATT
Cuts at: 0 433 927
Size: 433 494
StyI C'CwwG_G
Cuts at: 0 20 470 927
Size: 20 450 457
Fragments arranged by size:
457 450 20
XbaI T'CTAG_A
Cuts at: 0 712 927
Size: 712 215
XhoI C'TCGA_G
Cuts at: 0 638 927
Size: 638 289
XmnI GAAnn'nnTTC
Cuts at: 0 13 927
Size: 13 914
Enzymes that do cut:
AccI AflII AflIII AleI AlwNI AvaI BanI BanII
BcgI BglII BsaI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspHI BstEII BstXI BstYI BtgI BtsI EcoICRI
EcoNI Hin4I HincII MmeI MslI MspA1I NspI PciI
PpiI PspXI PvuII SacI SfcI SmlI SspI StyI
XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TatI TstI Tth111I XcmI XmaI
ZraI