(Linear) (Six-base) MAPSORT of: KIBB5297.seq  Check: 7909  from: 1  to: 900



KIBB5297.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     275     900 
   Size:        275     625
 
AflIII A'CryG_T

Cuts at:      0     525     640     900 
   Size:        525     115     260

  Fragments arranged by size: 

                 525     260     115
 
AlwNI CAG_nnn'CTG

Cuts at:      0     130     900 
   Size:        130     770
 
ApoI r'AATT_y

Cuts at:      0       4     163     900 
   Size:          4     159     737

  Fragments arranged by size: 

                 737     159       4
 
BamHI G'GATC_C

Cuts at:      0     812     900 
   Size:        812      88
 
BanI G'GyrC_C

Cuts at:      0     250     900 
   Size:        250     650
 
BfrBI ATG'CAT

Cuts at:      0     741     900 
   Size:        741     159
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     256     900 
   Size:        256     644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     647     900 
   Size:        238     409     253

  Fragments arranged by size: 

                 409     253     238
 
Bpu10I CC'TnA_GC

Cuts at:      0     821     900 
   Size:        821      79
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     102     900 
   Size:        102     798
 
BseYI C'CCAG_C

Cuts at:      0     130     900 
   Size:        130     770
 
BsrDI GCAATG_nn'

Cuts at:      0     479     900 
   Size:        479     421
 
BsrFI r'CCGG_y

Cuts at:      0     103     900 
   Size:        103     797
 
BstYI r'GATC_y

Cuts at:      0     399     812     900 
   Size:        399     413      88

  Fragments arranged by size: 

                 413     399      88
 
Bsu36I CC'TnA_GG

Cuts at:      0     716     900 
   Size:        716     184
 
BtgI C'CryG_G

Cuts at:      0     489     900 
   Size:        489     411
 
DraI TTT'AAA

Cuts at:      0     842     900 
   Size:        842      58
 
EaeI y'GGCC_r

Cuts at:      0     492     900 
   Size:        492     408
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     647     900 
   Size:        238     409     253

  Fragments arranged by size: 

                 409     253     238
 
EcoO109I rG'GnC_Cy

Cuts at:      0     700     900 
   Size:        700     200
 
HindIII A'AGCT_T

Cuts at:      0      70     900 
   Size:         70     830
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     558     900 
   Size:        558     342
 
MscI TGG'CCA

Cuts at:      0     494     900 
   Size:        494     406
 
MslI CAynn'nnrTG

Cuts at:      0     645     900 
   Size:        645     255
 
MspA1I CmG'CkG

Cuts at:      0     229     520     900 
   Size:        229     291     380

  Fragments arranged by size: 

                 380     291     229
 
NcoI C'CATG_G

Cuts at:      0     489     900 
   Size:        489     411
 
NsiI A_TGCA'T

Cuts at:      0     743     900 
   Size:        743     157
 
NspI r_CATG'y

Cuts at:      0     529     644     900 
   Size:        529     115     256

  Fragments arranged by size: 

                 529     256     115
 
PciI A'CATG_T

Cuts at:      0     525     640     900 
   Size:        525     115     260

  Fragments arranged by size: 

                 525     260     115
 
PpuMI rG'GwC_Cy

Cuts at:      0     700     900 
   Size:        700     200
 
PstI C_TGCA'G

Cuts at:      0     283     900 
   Size:        283     617
 
PvuII CAG'CTG

Cuts at:      0     229     520     900 
   Size:        229     291     380

  Fragments arranged by size: 

                 380     291     229
 
SanDI GG'GwC_CC

Cuts at:      0     700     900 
   Size:        700     200
 
SfcI C'TryA_G

Cuts at:      0     279     785     900 
   Size:        279     506     115

  Fragments arranged by size: 

                 506     279     115
 
SgrAI Cr'CCGG_yG

Cuts at:      0     103     900 
   Size:        103     797
 
StyI C'CwwG_G

Cuts at:      0     254     489     900 
   Size:        254     235     411

  Fragments arranged by size: 

                 411     254     235
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     238     522     900 
   Size:        238     284     378

  Fragments arranged by size: 

                 378     284     238
 
TatI w'GTAC_w

Cuts at:      0     432     688     900 
   Size:        432     256     212

  Fragments arranged by size: 

                 432     256     212
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApoI    BamHI     BanI    BfrBI     BmrI
     BpmI   Bpu10I    BseRI    BseYI    BsrDI    BsrFI    BstYI   Bsu36I
     BtgI     DraI     EaeI  Eco57MI EcoO109I  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI     NspI     PciI    PpuMI     PstI
    PvuII    SanDI     SfcI    SgrAI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI