(Linear) (Six-base) MAPSORT of: KIBB5291.seq  Check: 6665  from: 1  to: 507



KIBB5291.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AccI GT'mk_AC

Cuts at:      0     278     507 
   Size:        278     229
 
AlwNI CAG_nnn'CTG

Cuts at:      0      14     507 
   Size:         14     493
 
ApaI G_GGCC'C

Cuts at:      0     206     507 
   Size:        206     301
 
ApoI r'AATT_y

Cuts at:      0      29     507 
   Size:         29     478
 
AvaI C'yCGr_G

Cuts at:      0     175     507 
   Size:        175     332
 
BanI G'GyrC_C

Cuts at:      0       3      17     507 
   Size:          3      14     490

  Fragments arranged by size: 

                 490      14       3
 
BanII G_rGCy'C

Cuts at:      0     206     507 
   Size:        206     301
 
Bme1580I G_kGCm'C

Cuts at:      0      22     206     507 
   Size:         22     184     301

  Fragments arranged by size: 

                 301     184      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     507 
   Size:        304     203
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     507 
   Size:        202     305
 
BseYI C'CCAG_C

Cuts at:      0      21     507 
   Size:         21     486
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     507 
   Size:        470      37
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     206     507 
   Size:         22     184     301

  Fragments arranged by size: 

                 301     184      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     253     507 
   Size:        253     254
 
BsrDI GCAATG_nn'

Cuts at:      0      77     507 
   Size:         77     430
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     213     252     507 
   Size:        213      39     255

  Fragments arranged by size: 

                 255     213      39
 
BstYI r'GATC_y

Cuts at:      0     145     507 
   Size:        145     362
 
BtgI C'CryG_G

Cuts at:      0     206     507 
   Size:        206     301
 
EarI CTCTTCn'nnn_

Cuts at:      0      72     507 
   Size:         72     435
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     507 
   Size:        304     203
 
EcoO109I rG'GnC_Cy

Cuts at:      0     202     507 
   Size:        202     305
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     339     371     427     459     507 
   Size:        339      32      56      32      48

  Fragments arranged by size: 

                 339      56      48      32      32
 
HincII GTy'rAC

Cuts at:      0     279     507 
   Size:        279     228
 
MslI CAynn'nnrTG

Cuts at:      0     109     211     507 
   Size:        109     102     296

  Fragments arranged by size: 

                 296     109     102
 
NcoI C'CATG_G

Cuts at:      0     206     507 
   Size:        206     301
 
NspI r_CATG'y

Cuts at:      0     403     507 
   Size:        403     104
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      14     507 
   Size:         14     493
 
PfoI T'CCnGG_A

Cuts at:      0     438     507 
   Size:        438      69
 
PsiI TTA'TAA

Cuts at:      0     469     507 
   Size:        469      38
 
PspOMI G'GGCC_C

Cuts at:      0     202     507 
   Size:        202     305
 
SalI G'TCGA_C

Cuts at:      0     277     507 
   Size:        277     230
 
SapI GCTCTTCn'nnn_

Cuts at:      0      72     507 
   Size:         72     435
 
SexAI A'CCwGG_T

Cuts at:      0      13     507 
   Size:         13     494
 
StyI C'CwwG_G

Cuts at:      0     206     507 
   Size:        206     301
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     507 
   Size:         21     486
 
XbaI T'CTAG_A

Cuts at:      0     228     507 
   Size:        228     279
 
XmnI GAAnn'nnTTC

Cuts at:      0     429     507 
   Size:        429      78
 

 Enzymes that do cut:

     AccI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII Bme1580I
     BpmI    BseRI    BseYI     BsgI Bsp1286I    BspMI    BsrDI    BstXI
    BstYI     BtgI     EarI  Eco57MI EcoO109I    Hin4I   HincII     MslI
     NcoI     NspI    PflMI     PfoI     PsiI   PspOMI     SalI     SapI
    SexAI     StyI    TaqII     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI