(Linear) (Six-base) MAPSORT of: KIBB5282.seq  Check: 2112  from: 1  to: 675



KIBB5282.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     675 
   Size:        600      75
 
AflII C'TTAA_G

Cuts at:      0     265     675 
   Size:        265     410
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424     675 
   Size:        392      32     251

  Fragments arranged by size: 

                 392     251      32
 
ApoI r'AATT_y

Cuts at:      0      78     238     282     675 
   Size:         78     160      44     393

  Fragments arranged by size: 

                 393     160      78      44
 
BanI G'GyrC_C

Cuts at:      0      21     675 
   Size:         21     654
 
BbeI G_GCGC'C

Cuts at:      0      25     675 
   Size:         25     650
 
BglII A'GATC_T

Cuts at:      0     621     675 
   Size:        621      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     123     446     675 
   Size:        123     323     229

  Fragments arranged by size: 

                 323     229     123
 
BsaAI yAC'GTr

Cuts at:      0      89     675 
   Size:         89     586
 
BsaBI GATnn'nnATC

Cuts at:      0     626     675 
   Size:        626      49
 
BsaHI Gr'CG_yC

Cuts at:      0      22      41     675 
   Size:         22      19     634

  Fragments arranged by size: 

                 634      22      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     675 
   Size:         31     644
 
BsiHKAI G_wGCw'C

Cuts at:      0     653     675 
   Size:        653      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     653     675 
   Size:        653      22
 
BsrBI CCG'CTC

Cuts at:      0     162     675 
   Size:        162     513
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     510     675 
   Size:        510     165
 
BstYI r'GATC_y

Cuts at:      0     621     675 
   Size:        621      54
 
BtgI C'CryG_G

Cuts at:      0     350     675 
   Size:        350     325
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     392     675 
   Size:        392     283
 
ClaI AT'CG_AT

Cuts at:      0     291     675 
   Size:        291     384
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     208     675 
   Size:        208     467
 
EaeI y'GGCC_r

Cuts at:      0       2      61     167     675 
   Size:          2      59     106     508

  Fragments arranged by size: 

                 508     106      59       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     511     675 
   Size:        511     164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     675 
   Size:        600      75
 
HaeII r_GCGC'y

Cuts at:      0      25     675 
   Size:         25     650
 
KasI G'GCGC_C

Cuts at:      0      21     675 
   Size:         21     654
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     675 
   Size:        411     264
 
MscI TGG'CCA

Cuts at:      0      63     169     675 
   Size:         63     106     506

  Fragments arranged by size: 

                 506     106      63
 
MslI CAynn'nnrTG

Cuts at:      0     104     675 
   Size:        104     571
 
NarI GG'CG_CC

Cuts at:      0      22     675 
   Size:         22     653
 
NcoI C'CATG_G

Cuts at:      0     350     675 
   Size:        350     325
 
NdeI CA'TA_TG

Cuts at:      0     634     675 
   Size:        634      41
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     351     675 
   Size:        351     324
 
SfoI GGC'GCC

Cuts at:      0      23     675 
   Size:         23     652
 
SmlI C'TyrA_G

Cuts at:      0     265     675 
   Size:        265     410
 
StyI C'CwwG_G

Cuts at:      0     170     350     675 
   Size:        170     180     325

  Fragments arranged by size: 

                 325     180     170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     343     675 
   Size:        343     332
 
XmnI GAAnn'nnTTC

Cuts at:      0      82     675 
   Size:         82     593
 

 Enzymes that do cut:

     AcuI    AflII     AloI     ApoI     BanI     BbeI    BglII     BmrI
    BsaAI    BsaBI    BsaHI    BseRI  BsiHKAI Bsp1286I    BsrBI    BstXI
    BstYI     BtgI    BtgZI     ClaI     DrdI     EaeI     EarI  Eco57MI
    HaeII     KasI     MmeI     MscI     MslI     NarI     NcoI     NdeI
    PflMI     SfoI     SmlI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI   MspA1I     NaeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI