(Linear) (Six-base) MAPSORT of: KIBB5280.seq  Check: 9275  from: 1  to: 738



KIBB5280.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     738 
   Size:        470     268
 
AfeI AGC'GCT

Cuts at:      0      55     738 
   Size:         55     683
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21     675     738 
   Size:         21     654      63

  Fragments arranged by size: 

                 654      63      21
 
BanI G'GyrC_C

Cuts at:      0     160     738 
   Size:        160     578
 
BclI T'GATC_A

Cuts at:      0     323     662     738 
   Size:        323     339      76

  Fragments arranged by size: 

                 339     323      76
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     738 
   Size:         46     692
 
BlpI GC'TnA_GC

Cuts at:      0     617     738 
   Size:        617     121
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     703     738 
   Size:        703      35
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     298     738 
   Size:        198     100     440

  Fragments arranged by size: 

                 440     198     100
 
Bpu10I CC'TnA_GC

Cuts at:      0     569     738 
   Size:        569     169
 
BsaBI GATnn'nnATC

Cuts at:      0     125     738 
   Size:        125     613
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      55      85     738 
   Size:         55      30     653

  Fragments arranged by size: 

                 653      55      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     738 
   Size:        121     617
 
BseYI C'CCAG_C

Cuts at:      0     516     738 
   Size:        516     222
 
BsmI GAATG_Cn'

Cuts at:      0     567     738 
   Size:        567     171
 
BsrDI GCAATG_nn'

Cuts at:      0     297     342     738 
   Size:        297      45     396

  Fragments arranged by size: 

                 396     297      45
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      21     675     738 
   Size:         21     654      63

  Fragments arranged by size: 

                 654      63      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     516     738 
   Size:        516     222
 
BstYI r'GATC_y

Cuts at:      0     554     738 
   Size:        554     184
 
BtgI C'CryG_G

Cuts at:      0     209     738 
   Size:        209     529
 
EaeI y'GGCC_r

Cuts at:      0      38     738 
   Size:         38     700
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64     738 
   Size:         64     674
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     298     470     738 
   Size:        198     100     172     268

  Fragments arranged by size: 

                 268     198     172     100
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     486     738 
   Size:        486     252
 
HaeII r_GCGC'y

Cuts at:      0      57     738 
   Size:         57     681
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     286     318     738 
   Size:         55      32     199      32     420

  Fragments arranged by size: 

                 420     199      55      32      32
 
HindIII A'AGCT_T

Cuts at:      0     248     443     738 
   Size:        248     195     295

  Fragments arranged by size: 

                 295     248     195
 
MspA1I CmG'CkG

Cuts at:      0      18      27     738 
   Size:         18       9     711

  Fragments arranged by size: 

                 711      18       9
 
NcoI C'CATG_G

Cuts at:      0     209     738 
   Size:        209     529
 
PfoI T'CCnGG_A

Cuts at:      0     175     738 
   Size:        175     563
 
PstI C_TGCA'G

Cuts at:      0      26     738 
   Size:         26     712
 
PvuII CAG'CTG

Cuts at:      0      18     738 
   Size:         18     720
 
SfcI C'TryA_G

Cuts at:      0      22     738 
   Size:         22     716
 
StyI C'CwwG_G

Cuts at:      0     209     738 
   Size:        209     529
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     618     738 
   Size:        618     120
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     BanI     BclI     BglI     BlpI     BplI
     BpmI   Bpu10I    BsaBI    BsaXI    BseRI    BseYI     BsmI    BsrDI
   BstAPI    BstXI    BstYI     BtgI     EaeI     EciI  Eco57MI     FalI
    HaeII    Hin4I  HindIII   MspA1I     NcoI     PfoI     PstI    PvuII
     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI