(Linear) (Six-base) MAPSORT of: KIBB5266.seq  Check: 5417  from: 1  to: 1050



KIBB5266.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      34    1050 
   Size:         34    1016
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     416     944     986    1013    1050 
   Size:         48     368     528      42      27      37

  Fragments arranged by size: 

                 528     368      48      42      37      27
 
AfeI AGC'GCT

Cuts at:      0     102    1050 
   Size:        102     948
 
AlwNI CAG_nnn'CTG

Cuts at:      0     232    1036    1050 
   Size:        232     804      14

  Fragments arranged by size: 

                 804     232      14
 
ApoI r'AATT_y

Cuts at:      0     540     706    1008    1050 
   Size:        540     166     302      42

  Fragments arranged by size: 

                 540     302     166      42
 
BsaHI Gr'CG_yC

Cuts at:      0      16    1050 
   Size:         16    1034
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     997    1050 
   Size:        997      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     650    1050 
   Size:        474     176     400

  Fragments arranged by size: 

                 474     400     176
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      34    1050 
   Size:         34    1016
 
BsrBI CCG'CTC

Cuts at:      0      21    1050 
   Size:         21    1029
 
BsrDI GCAATG_nn'

Cuts at:      0      11    1050 
   Size:         11    1039
 
Bsu36I CC'TnA_GG

Cuts at:      0     407     578    1050 
   Size:        407     171     472

  Fragments arranged by size: 

                 472     407     171
 
BtgI C'CryG_G

Cuts at:      0      47    1050 
   Size:         47    1003
 
BtsI GCAGTG_nn'

Cuts at:      0     462    1050 
   Size:        462     588
 
DraI TTT'AAA

Cuts at:      0     735    1050 
   Size:        735     315
 
DraIII CAC_nnn'GTG

Cuts at:      0     810    1050 
   Size:        810     240
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     794    1050 
   Size:        794     256
 
EaeI y'GGCC_r

Cuts at:      0      33    1050 
   Size:         33    1017
 
EarI CTCTTCn'nnn_

Cuts at:      0     695     974    1050 
   Size:        695     279      76

  Fragments arranged by size: 

                 695     279      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     416     944     986    1013    1050 
   Size:         48     368     528      42      27      37

  Fragments arranged by size: 

                 528     368      48      42      37      27
 
EcoRI G'AATT_C

Cuts at:      0    1008    1050 
   Size:       1008      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     727     807    1050 
   Size:        727      80     243

  Fragments arranged by size: 

                 727     243      80
 
HaeII r_GCGC'y

Cuts at:      0     104    1050 
   Size:        104     946
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     276     282     308     804     836    1050 
   Size:        250      26       6      26     496      32     214

  Fragments arranged by size: 

                 496     250     214      32      26      26       6
 
HincII GTy'rAC

Cuts at:      0     347    1050 
   Size:        347     703
 
HpaI GTT'AAC

Cuts at:      0     347    1050 
   Size:        347     703
 
MscI TGG'CCA

Cuts at:      0      35    1050 
   Size:         35    1015
 
MslI CAynn'nnrTG

Cuts at:      0     176    1050 
   Size:        176     874
 
MspA1I CmG'CkG

Cuts at:      0     232    1050 
   Size:        232     818
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     254     958    1050 
   Size:        254     704      92

  Fragments arranged by size: 

                 704     254      92
 
PsiI TTA'TAA

Cuts at:      0     598     743     755    1050 
   Size:        598     145      12     295

  Fragments arranged by size: 

                 598     295     145      12
 
PstI C_TGCA'G

Cuts at:      0     237    1050 
   Size:        237     813
 
PvuII CAG'CTG

Cuts at:      0     232    1050 
   Size:        232     818
 
SfcI C'TryA_G

Cuts at:      0     224     233    1050 
   Size:        224       9     817

  Fragments arranged by size: 

                 817     224       9
 
XmnI GAAnn'nnTTC

Cuts at:      0     572    1050 
   Size:        572     478
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApoI    BsaHI    BseRI     BsgI
    BspMI    BsrBI    BsrDI   Bsu36I     BtgI     BtsI     DraI   DraIII
     DrdI     EaeI     EarI  Eco57MI    EcoRI     FalI    HaeII    Hin4I
   HincII     HpaI     MscI     MslI   MspA1I    PflMI     PsiI     PstI
    PvuII     SfcI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI