Restriction map for
pF1KB5264
(Linear) (Six-base) MAPSORT of: KIBB5264.seq Check: 2213 from: 1 to: 936
KIBB5264.orf
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 0 898 936
Size: 898 38
AfeI AGC'GCT
Cuts at: 0 9 936
Size: 9 927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 936
Size: 592 344
ApoI r'AATT_y
Cuts at: 0 289 936
Size: 289 647
AseI AT'TA_AT
Cuts at: 0 479 936
Size: 479 457
AvaI C'yCGr_G
Cuts at: 0 138 936
Size: 138 798
BanI G'GyrC_C
Cuts at: 0 49 936
Size: 49 887
BanII G_rGCy'C
Cuts at: 0 146 838 909 936
Size: 146 692 71 27
Fragments arranged by size:
692 146 71 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 428 805 936
Size: 428 377 131
Fragments arranged by size:
428 377 131
BclI T'GATC_A
Cuts at: 0 458 936
Size: 458 478
Bme1580I G_kGCm'C
Cuts at: 0 52 936
Size: 52 884
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 936
Size: 45 891
BsaHI Gr'CG_yC
Cuts at: 0 671 936
Size: 671 265
BsaWI w'CCGG_w
Cuts at: 0 725 753 936
Size: 725 28 183
Fragments arranged by size:
725 183 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 936
Size: 667 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 936
Size: 700 236
BsmBI CGTCTCn'nnnn_
Cuts at: 0 751 936
Size: 751 185
Bsp1286I G_dGCh'C
Cuts at: 0 52 146 838 909 936
Size: 52 94 692 71 27
Fragments arranged by size:
692 94 71 52 27
BspEI T'CCGG_A
Cuts at: 0 725 753 936
Size: 725 28 183
Fragments arranged by size:
725 183 28
BspHI T'CATG_A
Cuts at: 0 881 936
Size: 881 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 860 936
Size: 860 76
BsrDI GCAATG_nn'
Cuts at: 0 311 936
Size: 311 625
BsrFI r'CCGG_y
Cuts at: 0 22 936
Size: 22 914
BstYI r'GATC_y
Cuts at: 0 535 936
Size: 535 401
BtgI C'CryG_G
Cuts at: 0 53 936
Size: 53 883
BtsI GCAGTG_nn'
Cuts at: 0 576 936
Size: 576 360
EarI CTCTTCn'nnn_
Cuts at: 0 94 197 936
Size: 94 103 739
Fragments arranged by size:
739 103 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 936
Size: 649 287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 690 936
Size: 690 246
HaeII r_GCGC'y
Cuts at: 0 11 936
Size: 11 925
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 619 651 733 765 803 810
Size: 313 32 274 32 82 32 38 7
Cuts at: 810 835 842 936
Size: 25 7 94
Fragments arranged by size:
313 274 94 82 38 32 32 32
25 7 7
HincII GTy'rAC
Cuts at: 0 321 936
Size: 321 615
MfeI C'AATT_G
Cuts at: 0 508 936
Size: 508 428
MslI CAynn'nnrTG
Cuts at: 0 388 886 936
Size: 388 498 50
Fragments arranged by size:
498 388 50
NaeI GCC'GGC
Cuts at: 0 24 936
Size: 24 912
NdeI CA'TA_TG
Cuts at: 0 195 936
Size: 195 741
NgoMIV G'CCGG_C
Cuts at: 0 22 936
Size: 22 914
NspI r_CATG'y
Cuts at: 0 579 936
Size: 579 357
SapI GCTCTTCn'nnn_
Cuts at: 0 94 936
Size: 94 842
ScaI AGT'ACT
Cuts at: 0 174 936
Size: 174 762
SexAI A'CCwGG_T
Cuts at: 0 263 936
Size: 263 673
SmaI CCC'GGG
Cuts at: 0 140 936
Size: 140 796
SphI G_CATG'C
Cuts at: 0 579 936
Size: 579 357
SspI AAT'ATT
Cuts at: 0 477 483 936
Size: 477 6 453
Fragments arranged by size:
477 453 6
TatI w'GTAC_w
Cuts at: 0 32 172 936
Size: 32 140 764
Fragments arranged by size:
764 140 32
XmaI C'CCGG_G
Cuts at: 0 138 936
Size: 138 798
Enzymes that do cut:
AccI AfeI AlfI ApoI AseI AvaI BanI BanII
BciVI BclI Bme1580I BmrI BsaHI BsaWI BseRI BsgI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstYI
BtgI BtsI EarI EcoNI FalI HaeII Hin4I HincII
MfeI MslI NaeI NdeI NgoMIV NspI SapI ScaI
SexAI SmaI SphI SspI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MluI MmeI MscI MspA1I NarI NcoI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI