Restriction map for
pF1KB5258
(Linear) (Six-base) MAPSORT of: KIBB5258.seq Check: 4923 from: 1 to: 972
KIBB5258.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 612 690 972
Size: 612 78 282
Fragments arranged by size:
612 282 78
Acc65I G'GTAC_C
Cuts at: 0 839 972
Size: 839 133
AhdI GACnn_n'nnGTC
Cuts at: 0 815 972
Size: 815 157
AlwNI CAG_nnn'CTG
Cuts at: 0 382 972
Size: 382 590
ApaI G_GGCC'C
Cuts at: 0 745 972
Size: 745 227
ApoI r'AATT_y
Cuts at: 0 933 972
Size: 933 39
AvaI C'yCGr_G
Cuts at: 0 114 946 972
Size: 114 832 26
Fragments arranged by size:
832 114 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 823 856 972
Size: 823 33 116
Fragments arranged by size:
823 116 33
BanI G'GyrC_C
Cuts at: 0 135 839 972
Size: 135 704 133
Fragments arranged by size:
704 135 133
BanII G_rGCy'C
Cuts at: 0 745 972
Size: 745 227
BbeI G_GCGC'C
Cuts at: 0 139 972
Size: 139 833
BbvCI CC'TCA_GC
Cuts at: 0 58 972
Size: 58 914
BglI GCCn_nnn'nGGC
Cuts at: 0 119 972
Size: 119 853
BlpI GC'TnA_GC
Cuts at: 0 953 972
Size: 953 19
Bme1580I G_kGCm'C
Cuts at: 0 745 972
Size: 745 227
BmgBI CAC'GTC
Cuts at: 0 448 972
Size: 448 524
Bpu10I CC'TnA_GC
Cuts at: 0 58 972
Size: 58 914
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 591 802 972
Size: 10 581 211 170
Fragments arranged by size:
581 211 170 10
BsaHI Gr'CG_yC
Cuts at: 0 136 609 870 972
Size: 136 473 261 102
Fragments arranged by size:
473 261 136 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 972
Size: 308 30 634
Fragments arranged by size:
634 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 737 972
Size: 737 235
BseYI C'CCAG_C
Cuts at: 0 20 972
Size: 20 952
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 695 972
Size: 658 37 277
Fragments arranged by size:
658 277 37
BsiHKAI G_wGCw'C
Cuts at: 0 531 972
Size: 531 441
Bsp1286I G_dGCh'C
Cuts at: 0 531 745 972
Size: 531 214 227
Fragments arranged by size:
531 227 214
BsrBI CCG'CTC
Cuts at: 0 107 943 972
Size: 107 836 29
Fragments arranged by size:
836 107 29
BsrDI GCAATG_nn'
Cuts at: 0 783 972
Size: 783 189
BsrFI r'CCGG_y
Cuts at: 0 142 554 972
Size: 142 412 418
Fragments arranged by size:
418 412 142
BsrGI T'GTAC_A
Cuts at: 0 255 972
Size: 255 717
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 147 972
Size: 147 825
BtsI GCAGTG_nn'
Cuts at: 0 309 972
Size: 309 663
DrdI GACnn_nn'nnGTC
Cuts at: 0 157 972
Size: 157 815
EcoNI CCTnn'n_nnAGG
Cuts at: 0 564 972
Size: 564 408
EcoO109I rG'GnC_Cy
Cuts at: 0 379 457 741 742 972
Size: 379 78 284 1 230
Fragments arranged by size:
379 284 230 78 1
EcoRI G'AATT_C
Cuts at: 0 933 972
Size: 933 39
HaeII r_GCGC'y
Cuts at: 0 65 139 972
Size: 65 74 833
Fragments arranged by size:
833 74 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 801 833 922 954 972
Size: 801 32 89 32 18
Fragments arranged by size:
801 89 32 32 18
HincII GTy'rAC
Cuts at: 0 613 972
Size: 613 359
KasI G'GCGC_C
Cuts at: 0 135 972
Size: 135 837
KpnI G_GTAC'C
Cuts at: 0 843 972
Size: 843 129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 972
Size: 650 322
MspA1I CmG'CkG
Cuts at: 0 95 928 972
Size: 95 833 44
Fragments arranged by size:
833 95 44
NaeI GCC'GGC
Cuts at: 0 144 556 972
Size: 144 412 416
Fragments arranged by size:
416 412 144
NarI GG'CG_CC
Cuts at: 0 136 972
Size: 136 836
NgoMIV G'CCGG_C
Cuts at: 0 142 554 972
Size: 142 412 418
Fragments arranged by size:
418 412 142
NspI r_CATG'y
Cuts at: 0 594 972
Size: 594 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 895 972
Size: 895 77
PpuMI rG'GwC_Cy
Cuts at: 0 379 457 972
Size: 379 78 515
Fragments arranged by size:
515 379 78
PspOMI G'GGCC_C
Cuts at: 0 741 972
Size: 741 231
PvuII CAG'CTG
Cuts at: 0 928 972
Size: 928 44
SalI G'TCGA_C
Cuts at: 0 611 972
Size: 611 361
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 119 972
Size: 119 853
SfoI GGC'GCC
Cuts at: 0 137 972
Size: 137 835
SmaI CCC'GGG
Cuts at: 0 116 972
Size: 116 856
SrfI GCCC'GGGC
Cuts at: 0 116 972
Size: 116 856
StyI C'CwwG_G
Cuts at: 0 239 813 972
Size: 239 574 159
Fragments arranged by size:
574 239 159
TatI w'GTAC_w
Cuts at: 0 255 972
Size: 255 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 972
Size: 308 32 632
Fragments arranged by size:
632 308 32
XmaI C'CCGG_G
Cuts at: 0 114 972
Size: 114 858
Enzymes that do cut:
AccI Acc65I AhdI AlwNI ApaI ApoI AvaI BaeI
BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI
Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrBI BsrDI BsrFI BsrGI BtgZI BtsI DrdI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI
MmeI MspA1I NaeI NarI NgoMIV NspI PflMI PpuMI
PspOMI PvuII SalI SfiI SfoI SmaI SrfI StyI
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BamHI BbsI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BplI BpmI BpuEI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI
ZraI