Restriction map for
pF1KB5251
(Linear) (Six-base) MAPSORT of: KIBB5251.seq Check: 9106 from: 1 to: 825
KIBB5251.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 561 825
Size: 561 264
AccI GT'mk_AC
Cuts at: 0 119 825
Size: 119 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 634 825
Size: 260 374 191
Fragments arranged by size:
374 260 191
AfeI AGC'GCT
Cuts at: 0 77 825
Size: 77 748
AflIII A'CryG_T
Cuts at: 0 122 825
Size: 122 703
AlwNI CAG_nnn'CTG
Cuts at: 0 102 606 739 825
Size: 102 504 133 86
Fragments arranged by size:
504 133 102 86
AvaI C'yCGr_G
Cuts at: 0 454 825
Size: 454 371
BanI G'GyrC_C
Cuts at: 0 742 825
Size: 742 83
BanII G_rGCy'C
Cuts at: 0 791 825
Size: 791 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 825
Size: 392 433
BbvCI CC'TCA_GC
Cuts at: 0 724 825
Size: 724 101
BciVI GTATCCnnnnn_n'
Cuts at: 0 340 825
Size: 340 485
BglI GCCn_nnn'nGGC
Cuts at: 0 61 70 825
Size: 61 9 755
Fragments arranged by size:
755 61 9
BmgBI CAC'GTC
Cuts at: 0 417 825
Size: 417 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 370 825
Size: 124 246 455
Fragments arranged by size:
455 246 124
Bpu10I CC'TnA_GC
Cuts at: 0 704 724 825
Size: 704 20 101
Fragments arranged by size:
704 101 20
BsaAI yAC'GTr
Cuts at: 0 123 825
Size: 123 702
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 825
Size: 580 245
BseYI C'CCAG_C
Cuts at: 0 606 760 825
Size: 606 154 65
Fragments arranged by size:
606 154 65
Bsp1286I G_dGCh'C
Cuts at: 0 791 825
Size: 791 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 561 825
Size: 561 264
BsrDI GCAATG_nn'
Cuts at: 0 506 825
Size: 506 319
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 825
Size: 45 780
BtsI GCAGTG_nn'
Cuts at: 0 347 825
Size: 347 478
EaeI y'GGCC_r
Cuts at: 0 62 80 825
Size: 62 18 745
Fragments arranged by size:
745 62 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 260 370 634 825
Size: 124 136 110 264 191
Fragments arranged by size:
264 191 136 124 110
EcoO109I rG'GnC_Cy
Cuts at: 0 108 495 699 825
Size: 108 387 204 126
Fragments arranged by size:
387 204 126 108
HaeII r_GCGC'y
Cuts at: 0 79 492 756 803 825
Size: 79 413 264 47 22
Fragments arranged by size:
413 264 79 47 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 825
Size: 166 32 627
Fragments arranged by size:
627 166 32
HincII GTy'rAC
Cuts at: 0 462 825
Size: 462 363
HindIII A'AGCT_T
Cuts at: 0 283 825
Size: 283 542
MscI TGG'CCA
Cuts at: 0 82 825
Size: 82 743
MspA1I CmG'CkG
Cuts at: 0 381 450 606 825
Size: 381 69 156 219
Fragments arranged by size:
381 219 156 69
NdeI CA'TA_TG
Cuts at: 0 336 825
Size: 336 489
NspI r_CATG'y
Cuts at: 0 147 825
Size: 147 678
PflMI CCAn_nnn'nTGG
Cuts at: 0 739 809 825
Size: 739 70 16
Fragments arranged by size:
739 70 16
PpuMI rG'GwC_Cy
Cuts at: 0 495 699 825
Size: 495 204 126
Fragments arranged by size:
495 204 126
PspXI vC'TCGA_Gb
Cuts at: 0 454 825
Size: 454 371
PstI C_TGCA'G
Cuts at: 0 524 825
Size: 524 301
PvuII CAG'CTG
Cuts at: 0 381 606 825
Size: 381 225 219
Fragments arranged by size:
381 225 219
SbfI CC_TGCA'GG
Cuts at: 0 524 825
Size: 524 301
SfcI C'TryA_G
Cuts at: 0 465 520 825
Size: 465 55 305
Fragments arranged by size:
465 305 55
SmlI C'TyrA_G
Cuts at: 0 454 825
Size: 454 371
StyI C'CwwG_G
Cuts at: 0 65 825
Size: 65 760
XhoI C'TCGA_G
Cuts at: 0 454 825
Size: 454 371
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI AvaI BanI
BanII BbsI BbvCI BciVI BglI BmgBI BpmI Bpu10I
BsaAI BseRI BseYI Bsp1286I BspMI BsrDI BstAPI BtsI
EaeI Eco57MI EcoO109I HaeII Hin4I HincII HindIII MscI
MspA1I NdeI NspI PflMI PpuMI PspXI PstI PvuII
SbfI SfcI SmlI StyI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI