Restriction map for
pF1KB5235
(Linear) (Six-base) MAPSORT of: KIBB5235.seq Check: 9675 from: 1 to: 1527
KIBB5235.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 713 1527
Size: 713 814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 142 370 589 595 925 1527
Size: 22 120 228 219 6 330 602
Fragments arranged by size:
602 330 228 219 120 22 6
AhdI GACnn_n'nnGTC
Cuts at: 0 729 1527
Size: 729 798
AleI CACnn'nnGTG
Cuts at: 0 997 1527
Size: 997 530
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 833 865 1527
Size: 833 32 662
Fragments arranged by size:
833 662 32
ApoI r'AATT_y
Cuts at: 0 157 508 1527
Size: 157 351 1019
Fragments arranged by size:
1019 351 157
AvaI C'yCGr_G
Cuts at: 0 974 1527
Size: 974 553
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 269 302 1527
Size: 269 33 1225
Fragments arranged by size:
1225 269 33
BamHI G'GATC_C
Cuts at: 0 855 1527
Size: 855 672
BanI G'GyrC_C
Cuts at: 0 1128 1527
Size: 1128 399
BanII G_rGCy'C
Cuts at: 0 371 1052 1145 1527
Size: 371 681 93 382
Fragments arranged by size:
681 382 371 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 1481 1527
Size: 332 1149 46
Fragments arranged by size:
1149 332 46
BciVI GTATCCnnnnn_n'
Cuts at: 0 840 1527
Size: 840 687
BlpI GC'TnA_GC
Cuts at: 0 1144 1313 1527
Size: 1144 169 214
Fragments arranged by size:
1144 214 169
Bme1580I G_kGCm'C
Cuts at: 0 184 1527
Size: 184 1343
BmrI ACTGGGnnnn_n'
Cuts at: 0 1004 1527
Size: 1004 523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 989 1527
Size: 989 538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 835 1097 1527
Size: 40 795 262 430
Fragments arranged by size:
795 430 262 40
Bpu10I CC'TnA_GC
Cuts at: 0 964 1527
Size: 964 563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1396 1527
Size: 46 1350 131
Fragments arranged by size:
1350 131 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 893 1527
Size: 893 634
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 35 65 358 388 833 863 937 967
Size: 35 30 293 30 445 30 74 30
Cuts at: 967 1527
Size: 560
Fragments arranged by size:
560 445 293 74 35 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 109 112 696 1216 1381 1527
Size: 37 72 3 584 520 165 146
Fragments arranged by size:
584 520 165 146 72 37 3
BseYI C'CCAG_C
Cuts at: 0 132 480 535 1029 1300 1527
Size: 132 348 55 494 271 227
Fragments arranged by size:
494 348 271 227 132 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1229 1527
Size: 284 945 298
Fragments arranged by size:
945 298 284
BsiEI CG_ry'CG
Cuts at: 0 639 1527
Size: 639 888
BsiHKAI G_wGCw'C
Cuts at: 0 167 371 1527
Size: 167 204 1156
Fragments arranged by size:
1156 204 167
BsmI GAATG_Cn'
Cuts at: 0 644 1527
Size: 644 883
Bsp1286I G_dGCh'C
Cuts at: 0 167 184 371 1052 1145 1527
Size: 167 17 187 681 93 382
Fragments arranged by size:
681 382 187 167 93 17
BsrBI CCG'CTC
Cuts at: 0 1161 1527
Size: 1161 366
BsrDI GCAATG_nn'
Cuts at: 0 425 530 1527
Size: 425 105 997
Fragments arranged by size:
997 425 105
BsrGI T'GTAC_A
Cuts at: 0 1322 1527
Size: 1322 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 1527
Size: 10 1517
BstXI CCAn_nnnn'nTGG
Cuts at: 0 820 1527
Size: 820 707
BstYI r'GATC_y
Cuts at: 0 590 855 1527
Size: 590 265 672
Fragments arranged by size:
672 590 265
Bsu36I CC'TnA_GG
Cuts at: 0 782 1527
Size: 782 745
BtgI C'CryG_G
Cuts at: 0 258 492 1217 1527
Size: 258 234 725 310
Fragments arranged by size:
725 310 258 234
BtsI GCAGTG_nn'
Cuts at: 0 1019 1071 1527
Size: 1019 52 456
Fragments arranged by size:
1019 456 52
DrdI GACnn_nn'nnGTC
Cuts at: 0 622 1527
Size: 622 905
EaeI y'GGCC_r
Cuts at: 0 495 1527
Size: 495 1032
EarI CTCTTCn'nnn_
Cuts at: 0 734 1040 1448 1464 1527
Size: 734 306 408 16 63
Fragments arranged by size:
734 408 306 63 16
EcoICRI GAG'CTC
Cuts at: 0 369 1527
Size: 369 1158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 40 142 370 589 595 835 925
Size: 22 18 102 228 219 6 240 90
Cuts at: 925 1097 1527
Size: 172 430
Fragments arranged by size:
430 240 228 219 172 102 90 22
18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 625 1483 1527
Size: 625 858 44
Fragments arranged by size:
858 625 44
EcoO109I rG'GnC_Cy
Cuts at: 0 136 771 891 1527
Size: 136 635 120 636
Fragments arranged by size:
636 635 136 120
EcoRI G'AATT_C
Cuts at: 0 508 1527
Size: 508 1019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 930 1527
Size: 900 30 597
Fragments arranged by size:
900 597 30
HaeII r_GCGC'y
Cuts at: 0 19 83 258 1527
Size: 19 64 175 1269
Fragments arranged by size:
1269 175 64 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 715 747 937 969 1527
Size: 358 32 325 32 190 32 558
Fragments arranged by size:
558 358 325 190 32 32 32
HindIII A'AGCT_T
Cuts at: 0 334 1276 1527
Size: 334 942 251
Fragments arranged by size:
942 334 251
MslI CAynn'nnrTG
Cuts at: 0 997 1153 1527
Size: 997 156 374
Fragments arranged by size:
997 374 156
MspA1I CmG'CkG
Cuts at: 0 132 846 1527
Size: 132 714 681
Fragments arranged by size:
714 681 132
NruI TCG'CGA
Cuts at: 0 218 1527
Size: 218 1309
NspI r_CATG'y
Cuts at: 0 1107 1527
Size: 1107 420
PasI CC'CwG_GG
Cuts at: 0 1053 1527
Size: 1053 474
PfoI T'CCnGG_A
Cuts at: 0 851 1527
Size: 851 676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 833 865 1527
Size: 833 32 662
Fragments arranged by size:
833 662 32
PpuMI rG'GwC_Cy
Cuts at: 0 136 1527
Size: 136 1391
PshAI GACnn'nnGTC
Cuts at: 0 194 1527
Size: 194 1333
PspXI vC'TCGA_Gb
Cuts at: 0 974 1527
Size: 974 553
PvuI CG_AT'CG
Cuts at: 0 639 1527
Size: 639 888
PvuII CAG'CTG
Cuts at: 0 132 846 1527
Size: 132 714 681
Fragments arranged by size:
714 681 132
SacI G_AGCT'C
Cuts at: 0 371 1527
Size: 371 1156
SapI GCTCTTCn'nnn_
Cuts at: 0 1040 1527
Size: 1040 487
ScaI AGT'ACT
Cuts at: 0 1360 1527
Size: 1360 167
SfcI C'TryA_G
Cuts at: 0 192 1021 1527
Size: 192 829 506
Fragments arranged by size:
829 506 192
SmlI C'TyrA_G
Cuts at: 0 61 974 1411 1527
Size: 61 913 437 116
Fragments arranged by size:
913 437 116 61
StuI AGG'CCT
Cuts at: 0 59 883 1527
Size: 59 824 644
Fragments arranged by size:
824 644 59
StyI C'CwwG_G
Cuts at: 0 176 884 1527
Size: 176 708 643
Fragments arranged by size:
708 643 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1045 1527
Size: 1045 482
TatI w'GTAC_w
Cuts at: 0 1322 1358 1527
Size: 1322 36 169
Fragments arranged by size:
1322 169 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 1527
Size: 35 32 1460
Fragments arranged by size:
1460 35 32
Tth111I GACn'n_nGTC
Cuts at: 0 808 1527
Size: 808 719
XbaI T'CTAG_A
Cuts at: 0 648 1527
Size: 648 879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 820 1527
Size: 820 707
XhoI C'TCGA_G
Cuts at: 0 974 1527
Size: 974 553
Enzymes that do cut:
AccI AcuI AhdI AleI AloI ApoI AvaI BaeI
BamHI BanI BanII BbsI BciVI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HindIII MslI MspA1I NruI NspI PasI PfoI
PpiI PpuMI PshAI PspXI PvuI PvuII SacI SapI
ScaI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BbeI BbvCI BcgI BclI BfrBI BglI BglII BmgBI
BmtI BsaAI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI
BspHI BspMI BsrFI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI PacI PciI PflMI PmeI PmlI
PsiI PspOMI PsrI PstI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XmaI XmnI ZraI