(Linear) (Six-base) MAPSORT of: KIBB5220.seq  Check: 308  from: 1  to: 2106



KIBB5220.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1753    2106 
   Size:        901     852     353

  Fragments arranged by size: 

                 901     852     353
 
AflIII A'CryG_T

Cuts at:      0    1958    2106 
   Size:       1958     148
 
AleI CACnn'nnGTG

Cuts at:      0     880    2106 
   Size:        880    1226
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     898    2106 
   Size:        898    1208
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1147    1915    2106 
   Size:       1147     768     191

  Fragments arranged by size: 

                1147     768     191
 
ApoI r'AATT_y

Cuts at:      0     936    2106 
   Size:        936    1170
 
AseI AT'TA_AT

Cuts at:      0    1052    2106 
   Size:       1052    1054
 
AvaI C'yCGr_G

Cuts at:      0    1799    2106 
   Size:       1799     307
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1895    1928    2106 
   Size:       1895      33     178

  Fragments arranged by size: 

                1895     178      33
 
BanI G'GyrC_C

Cuts at:      0      59    2106 
   Size:         59    2047
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     904    1850    2106 
   Size:        904     946     256

  Fragments arranged by size: 

                 946     904     256
 
BbvCI CC'TCA_GC

Cuts at:      0    1478    2106 
   Size:       1478     628
 
BfrBI ATG'CAT

Cuts at:      0     717    2106 
   Size:        717    1389
 
BglII A'GATC_T

Cuts at:      0     747    1377    2106 
   Size:        747     630     729

  Fragments arranged by size: 

                 747     729     630
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1903    2106 
   Size:       1903     203
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     800    1753    2106 
   Size:        800     953     353

  Fragments arranged by size: 

                 953     800     353
 
Bpu10I CC'TnA_GC

Cuts at:      0    1478    2106 
   Size:       1478     628
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1582    2106 
   Size:       1582     524
 
BsaAI yAC'GTr

Cuts at:      0    1959    2106 
   Size:       1959     147
 
BsaWI w'CCGG_w

Cuts at:      0     304    2106 
   Size:        304    1802
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1306    1993    2106 
   Size:       1306     687     113

  Fragments arranged by size: 

                1306     687     113
 
BsmI GAATG_Cn'

Cuts at:      0     691    2106 
   Size:        691    1415
 
BsrFI r'CCGG_y

Cuts at:      0      62    2106 
   Size:         62    2044
 
BsrGI T'GTAC_A

Cuts at:      0     141    2106 
   Size:        141    1965
 
BstYI r'GATC_y

Cuts at:      0     747    1059    1377    1932    2106 
   Size:        747     312     318     555     174

  Fragments arranged by size: 

                 747     555     318     312     174
 
Bsu36I CC'TnA_GG

Cuts at:      0     927    2106 
   Size:        927    1179
 
BtgI C'CryG_G

Cuts at:      0     326    2106 
   Size:        326    1780
 
BtsI GCAGTG_nn'

Cuts at:      0     696    2106 
   Size:        696    1410
 
EaeI y'GGCC_r

Cuts at:      0    1469    2106 
   Size:       1469     637
 
EarI CTCTTCn'nnn_

Cuts at:      0    1175    2106 
   Size:       1175     931
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     800     901    1753    2106 
   Size:        800     101     852     353

  Fragments arranged by size: 

                 852     800     353     101
 
EcoRV GAT'ATC

Cuts at:      0    1152    2106 
   Size:       1152     954
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1399    1510    2106 
   Size:       1399     111     596

  Fragments arranged by size: 

                1399     596     111
 
HaeII r_GCGC'y

Cuts at:      0     798    2106 
   Size:        798    1308
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     524     556    1390    1422    1677    1709    1974    2006 
   Size:        524      32     834      32     255      32     265      32

Cuts at:   2006    2106 
   Size:        100

  Fragments arranged by size: 

                 834     524     265     255     100      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1461    2106 
   Size:       1461     645
 
HindIII A'AGCT_T

Cuts at:      0    1117    2106 
   Size:       1117     989
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1173    1722    2106 
   Size:       1173     549     384

  Fragments arranged by size: 

                1173     549     384
 
MscI TGG'CCA

Cuts at:      0    1471    2106 
   Size:       1471     635
 
MslI CAynn'nnrTG

Cuts at:      0     880    1838    2106 
   Size:        880     958     268

  Fragments arranged by size: 

                 958     880     268
 
NaeI GCC'GGC

Cuts at:      0      64    2106 
   Size:         64    2042
 
NcoI C'CATG_G

Cuts at:      0     326    2106 
   Size:        326    1780
 
NdeI CA'TA_TG

Cuts at:      0     294    2106 
   Size:        294    1812
 
NgoMIV G'CCGG_C

Cuts at:      0      62    2106 
   Size:         62    2044
 
NsiI A_TGCA'T

Cuts at:      0     719    2106 
   Size:        719    1387
 
NspI r_CATG'y

Cuts at:      0     717     721    2106 
   Size:        717       4    1385

  Fragments arranged by size: 

                1385     717       4
 
PmlI CAC'GTG

Cuts at:      0    1959    2106 
   Size:       1959     147
 
PsiI TTA'TAA

Cuts at:      0     230    2106 
   Size:        230    1876
 
PstI C_TGCA'G

Cuts at:      0    2105    2106 
   Size:       2105       1
 
SfcI C'TryA_G

Cuts at:      0     680    2101    2106 
   Size:        680    1421       5

  Fragments arranged by size: 

                1421     680       5
 
SmlI C'TyrA_G

Cuts at:      0    1561    2106 
   Size:       1561     545
 
SspI AAT'ATT

Cuts at:      0     963    1032    2106 
   Size:        963      69    1074

  Fragments arranged by size: 

                1074     963      69
 
StuI AGG'CCT

Cuts at:      0    1727    2106 
   Size:       1727     379
 
StyI C'CwwG_G

Cuts at:      0     326     837     857    2106 
   Size:        326     511      20    1249

  Fragments arranged by size: 

                1249     511     326      20
 
TatI w'GTAC_w

Cuts at:      0     141     261    1000    2106 
   Size:        141     120     739    1106

  Fragments arranged by size: 

                1106     739     141     120
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     467    2064    2106 
   Size:        335     132    1597      42

  Fragments arranged by size: 

                1597     335     132      42
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI     AvaI
     BaeI     BanI     BbsI    BbvCI    BfrBI    BglII     BmrI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaWI    BseRI     BsmI    BsrFI    BsrGI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoRV
     FalI    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI     PmlI     PsiI
     PstI     SfcI     SmlI     SspI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI
    BanII     BbeI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NarI
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI