Restriction map for
pF1KB5212
(Linear) (Six-base) MAPSORT of: KIBB5212.seq Check: 3641 from: 1 to: 1182
KIBB5212.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1099 1182
Size: 1099 83
AccI GT'mk_AC
Cuts at: 0 107 1182
Size: 107 1075
AflIII A'CryG_T
Cuts at: 0 659 996 1182
Size: 659 337 186
Fragments arranged by size:
659 337 186
AleI CACnn'nnGTG
Cuts at: 0 166 1182
Size: 166 1016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 355 1182
Size: 355 827
AlwNI CAG_nnn'CTG
Cuts at: 0 588 1182
Size: 588 594
ApoI r'AATT_y
Cuts at: 0 433 940 1182
Size: 433 507 242
Fragments arranged by size:
507 433 242
AvaI C'yCGr_G
Cuts at: 0 10 364 976 1004 1182
Size: 10 354 612 28 178
Fragments arranged by size:
612 354 178 28 10
BamHI G'GATC_C
Cuts at: 0 182 701 1182
Size: 182 519 481
Fragments arranged by size:
519 481 182
BanI G'GyrC_C
Cuts at: 0 339 1182
Size: 339 843
BanII G_rGCy'C
Cuts at: 0 983 1182
Size: 983 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 706 740 1182
Size: 706 34 442
Fragments arranged by size:
706 442 34
BclI T'GATC_A
Cuts at: 0 551 1182
Size: 551 631
BglII A'GATC_T
Cuts at: 0 882 1182
Size: 882 300
Bme1580I G_kGCm'C
Cuts at: 0 344 525 1182
Size: 344 181 657
Fragments arranged by size:
657 344 181
BmgBI CAC'GTC
Cuts at: 0 549 669 1182
Size: 549 120 513
Fragments arranged by size:
549 513 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 519 1182
Size: 519 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1182
Size: 164 1018
BsaI GGTCTCn'nnnn_
Cuts at: 0 619 1182
Size: 619 563
BsaBI GATnn'nnATC
Cuts at: 0 181 1182
Size: 181 1001
BsaHI Gr'CG_yC
Cuts at: 0 1065 1182
Size: 1065 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 1182
Size: 478 30 674
Fragments arranged by size:
674 478 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 1182
Size: 58 1124
BseYI C'CCAG_C
Cuts at: 0 135 907 1182
Size: 135 772 275
Fragments arranged by size:
772 275 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1182
Size: 295 887
Bsp1286I G_dGCh'C
Cuts at: 0 344 525 983 1182
Size: 344 181 458 199
Fragments arranged by size:
458 344 199 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 596 1099 1182
Size: 93 503 503 83
Fragments arranged by size:
503 503 93 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 126 885 1182
Size: 126 759 297
Fragments arranged by size:
759 297 126
BstYI r'GATC_y
Cuts at: 0 182 701 882 1182
Size: 182 519 181 300
Fragments arranged by size:
519 300 182 181
Bsu36I CC'TnA_GG
Cuts at: 0 962 1182
Size: 962 220
BtgI C'CryG_G
Cuts at: 0 347 1182
Size: 347 835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 266 698 1182
Size: 266 432 484
Fragments arranged by size:
484 432 266
BtsI GCAGTG_nn'
Cuts at: 0 1046 1182
Size: 1046 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 103 1182
Size: 103 1079
EaeI y'GGCC_r
Cuts at: 0 320 356 675 743 1182
Size: 320 36 319 68 439
Fragments arranged by size:
439 320 319 68 36
EarI CTCTTCn'nnn_
Cuts at: 0 215 820 1182
Size: 215 605 362
Fragments arranged by size:
605 362 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1105 1182
Size: 1105 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1182
Size: 164 1018
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 799 1182
Size: 298 501 383
Fragments arranged by size:
501 383 298
EcoO109I rG'GnC_Cy
Cuts at: 0 225 585 990 1182
Size: 225 360 405 192
Fragments arranged by size:
405 360 225 192
EcoRI G'AATT_C
Cuts at: 0 433 1182
Size: 433 749
FspI TGC'GCA
Cuts at: 0 847 1182
Size: 847 335
HincII GTy'rAC
Cuts at: 0 651 1182
Size: 651 531
MluI A'CGCG_T
Cuts at: 0 996 1182
Size: 996 186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 1182
Size: 521 661
MscI TGG'CCA
Cuts at: 0 358 677 745 1182
Size: 358 319 68 437
Fragments arranged by size:
437 358 319 68
MslI CAynn'nnrTG
Cuts at: 0 166 194 736 856 1182
Size: 166 28 542 120 326
Fragments arranged by size:
542 326 166 120 28
MspA1I CmG'CkG
Cuts at: 0 87 510 1182
Size: 87 423 672
Fragments arranged by size:
672 423 87
NspI r_CATG'y
Cuts at: 0 193 448 663 1158 1182
Size: 193 255 215 495 24
Fragments arranged by size:
495 255 215 193 24
PciI A'CATG_T
Cuts at: 0 659 1182
Size: 659 523
PfoI T'CCnGG_A
Cuts at: 0 533 770 1182
Size: 533 237 412
Fragments arranged by size:
533 412 237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 616 648 1182
Size: 358 32 226 32 534
Fragments arranged by size:
534 358 226 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 225 585 990 1182
Size: 225 360 405 192
Fragments arranged by size:
405 360 225 192
PstI C_TGCA'G
Cuts at: 0 92 800 1182
Size: 92 708 382
Fragments arranged by size:
708 382 92
PvuII CAG'CTG
Cuts at: 0 87 510 1182
Size: 87 423 672
Fragments arranged by size:
672 423 87
SbfI CC_TGCA'GG
Cuts at: 0 800 1182
Size: 800 382
ScaI AGT'ACT
Cuts at: 0 529 1015 1182
Size: 529 486 167
Fragments arranged by size:
529 486 167
SexAI A'CCwGG_T
Cuts at: 0 335 1182
Size: 335 847
SfcI C'TryA_G
Cuts at: 0 88 796 1182
Size: 88 708 386
Fragments arranged by size:
708 386 88
SmaI CCC'GGG
Cuts at: 0 1006 1182
Size: 1006 176
SphI G_CATG'C
Cuts at: 0 448 1158 1182
Size: 448 710 24
Fragments arranged by size:
710 448 24
StyI C'CwwG_G
Cuts at: 0 449 1182
Size: 449 733
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 127 661 1182
Size: 40 87 534 521
Fragments arranged by size:
534 521 87 40
TatI w'GTAC_w
Cuts at: 0 527 1013 1182
Size: 527 486 169
Fragments arranged by size:
527 486 169
Tth111I GACn'n_nGTC
Cuts at: 0 646 1182
Size: 646 536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 636 735 1182
Size: 333 303 99 447
Fragments arranged by size:
447 333 303 99
XmaI C'CCGG_G
Cuts at: 0 1004 1182
Size: 1004 178
XmnI GAAnn'nnTTC
Cuts at: 0 373 814 1182
Size: 373 441 368
Fragments arranged by size:
441 373 368
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApoI AvaI
BamHI BanI BanII BcgI BclI BglII Bme1580I BmgBI
BmrI BpmI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI Bsp1286I BspMI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HincII MluI MmeI MscI MslI MspA1I
NspI PciI PfoI PpiI PpuMI PstI PvuII SbfI
ScaI SexAI SfcI SmaI SphI StyI TaqII TatI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BciVI BfrBI BglI BlpI BmtI
BplI Bpu10I BpuEI BsaAI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI DraIII EagI EcoICRI EcoRV FalI FseI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TstI XbaI XhoI ZraI