Restriction map for
pF1KB5188
(Linear) (Six-base) MAPSORT of: KIBB5188.seq Check: 1862 from: 1 to: 1266
KIBB5188.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AatII G_ACGT'C
Cuts at: 0 916 1266
Size: 916 350
AccI GT'mk_AC
Cuts at: 0 1076 1266
Size: 1076 190
AleI CACnn'nnGTG
Cuts at: 0 691 736 1266
Size: 691 45 530
Fragments arranged by size:
691 530 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 801 814 967 1266
Size: 801 13 153 299
Fragments arranged by size:
801 299 153 13
AlwNI CAG_nnn'CTG
Cuts at: 0 62 131 814 1266
Size: 62 69 683 452
Fragments arranged by size:
683 452 69 62
ApaI G_GGCC'C
Cuts at: 0 714 1266
Size: 714 552
ApaLI G'TGCA_C
Cuts at: 0 552 1266
Size: 552 714
BamHI G'GATC_C
Cuts at: 0 896 1266
Size: 896 370
BanI G'GyrC_C
Cuts at: 0 349 1014 1261 1266
Size: 349 665 247 5
Fragments arranged by size:
665 349 247 5
BanII G_rGCy'C
Cuts at: 0 169 714 1266
Size: 169 545 552
Fragments arranged by size:
552 545 169
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 990 1266
Size: 86 904 276
Fragments arranged by size:
904 276 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 963 997 1266
Size: 963 34 269
Fragments arranged by size:
963 269 34
BglI GCCn_nnn'nGGC
Cuts at: 0 365 1266
Size: 365 901
BglII A'GATC_T
Cuts at: 0 471 1266
Size: 471 795
BlpI GC'TnA_GC
Cuts at: 0 168 1266
Size: 168 1098
Bme1580I G_kGCm'C
Cuts at: 0 556 714 1017 1266
Size: 556 158 303 249
Fragments arranged by size:
556 303 249 158
BmrI ACTGGGnnnn_n'
Cuts at: 0 1035 1266
Size: 1035 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 183 188 1266
Size: 183 5 1078
Fragments arranged by size:
1078 183 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1266
Size: 153 1113
BsaI GGTCTCn'nnnn_
Cuts at: 0 538 1266
Size: 538 728
BsaAI yAC'GTr
Cuts at: 0 891 1266
Size: 891 375
BsaHI Gr'CG_yC
Cuts at: 0 913 1266
Size: 913 353
BsaWI w'CCGG_w
Cuts at: 0 668 1266
Size: 668 598
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 764 794 1266
Size: 535 30 199 30 472
Fragments arranged by size:
535 472 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 287 466 1255 1266
Size: 287 179 789 11
Fragments arranged by size:
789 287 179 11
BseYI C'CCAG_C
Cuts at: 0 935 1010 1266
Size: 935 75 256
Fragments arranged by size:
935 256 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 797 1266
Size: 403 394 469
Fragments arranged by size:
469 403 394
BsiEI CG_ry'CG
Cuts at: 0 194 401 1266
Size: 194 207 865
Fragments arranged by size:
865 207 194
BsiHKAI G_wGCw'C
Cuts at: 0 169 556 1148 1266
Size: 169 387 592 118
Fragments arranged by size:
592 387 169 118
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1147 1266
Size: 1147 119
Bsp1286I G_dGCh'C
Cuts at: 0 169 556 714 1017 1148 1266
Size: 169 387 158 303 131 118
Fragments arranged by size:
387 303 169 158 131 118
BspEI T'CCGG_A
Cuts at: 0 668 1266
Size: 668 598
BspHI T'CATG_A
Cuts at: 0 500 1266
Size: 500 766
BsrDI GCAATG_nn'
Cuts at: 0 528 1266
Size: 528 738
BsrFI r'CCGG_y
Cuts at: 0 193 1266
Size: 193 1073
BsrGI T'GTAC_A
Cuts at: 0 1136 1266
Size: 1136 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 62 356 1266
Size: 62 294 910
Fragments arranged by size:
910 294 62
BstEII G'GTnAC_C
Cuts at: 0 966 1266
Size: 966 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 993 1101 1266
Size: 993 108 165
Fragments arranged by size:
993 165 108
BstYI r'GATC_y
Cuts at: 0 471 896 1266
Size: 471 425 370
Fragments arranged by size:
471 425 370
BtgI C'CryG_G
Cuts at: 0 383 569 614 1266
Size: 383 186 45 652
Fragments arranged by size:
652 383 186 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 966 1266
Size: 966 300
BtsI GCAGTG_nn'
Cuts at: 0 785 1266
Size: 785 481
DrdI GACnn_nn'nnGTC
Cuts at: 0 1044 1266
Size: 1044 222
EaeI y'GGCC_r
Cuts at: 0 566 617 635 683 1091 1266
Size: 566 51 18 48 408 175
Fragments arranged by size:
566 408 175 51 48 18
EarI CTCTTCn'nnn_
Cuts at: 0 660 1266
Size: 660 606
EcoICRI GAG'CTC
Cuts at: 0 167 1266
Size: 167 1099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1266
Size: 153 1113
EcoO109I rG'GnC_Cy
Cuts at: 0 711 1266
Size: 711 555
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 103 1266
Size: 103 1163
HaeII r_GCGC'y
Cuts at: 0 132 1266
Size: 132 1134
MscI TGG'CCA
Cuts at: 0 619 637 685 1266
Size: 619 18 48 581
Fragments arranged by size:
619 581 48 18
MslI CAynn'nnrTG
Cuts at: 0 331 568 691 736 1266
Size: 331 237 123 45 530
Fragments arranged by size:
530 331 237 123 45
MspA1I CmG'CkG
Cuts at: 0 59 1035 1118 1266
Size: 59 976 83 148
Fragments arranged by size:
976 148 83 59
NcoI C'CATG_G
Cuts at: 0 569 614 1266
Size: 569 45 652
Fragments arranged by size:
652 569 45
NspI r_CATG'y
Cuts at: 0 495 1266
Size: 495 771
PasI CC'CwG_GG
Cuts at: 0 1197 1266
Size: 1197 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 1266
Size: 516 750
PfoI T'CCnGG_A
Cuts at: 0 111 1266
Size: 111 1155
PspOMI G'GGCC_C
Cuts at: 0 710 1266
Size: 710 556
PstI C_TGCA'G
Cuts at: 0 905 1194 1266
Size: 905 289 72
Fragments arranged by size:
905 289 72
PvuII CAG'CTG
Cuts at: 0 1035 1118 1266
Size: 1035 83 148
Fragments arranged by size:
1035 148 83
SacI G_AGCT'C
Cuts at: 0 169 1266
Size: 169 1097
SfcI C'TryA_G
Cuts at: 0 901 1030 1190 1266
Size: 901 129 160 76
Fragments arranged by size:
901 160 129 76
SphI G_CATG'C
Cuts at: 0 495 1266
Size: 495 771
StyI C'CwwG_G
Cuts at: 0 569 614 1266
Size: 569 45 652
Fragments arranged by size:
652 569 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 1266
Size: 144 1122
TatI w'GTAC_w
Cuts at: 0 1136 1266
Size: 1136 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 764 796 1266
Size: 535 32 197 32 470
Fragments arranged by size:
535 470 197 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 1266
Size: 360 906
ZraI GAC'GTC
Cuts at: 0 914 1266
Size: 914 352
Enzymes that do cut:
AatII AccI AleI AlfI AlwNI ApaI ApaLI BamHI
BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII MscI MslI MspA1I NcoI NspI
PasI PflMI PfoI PspOMI PstI PvuII SacI SfcI
SphI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI
Bpu10I BpuEI BsaBI BsiWI BsmI BspMI BsrBI BssHII
BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI