Restriction map for
pF1KB5182
(Linear) (Six-base) MAPSORT of: KIBB5182.seq Check: 9171 from: 1 to: 1515
KIBB5182.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1451 1513 1515
Size: 1451 62 2
Fragments arranged by size:
1451 62 2
AflIII A'CryG_T
Cuts at: 0 179 1058 1515
Size: 179 879 457
Fragments arranged by size:
879 457 179
AleI CACnn'nnGTG
Cuts at: 0 1051 1515
Size: 1051 464
AlwNI CAG_nnn'CTG
Cuts at: 0 1089 1515
Size: 1089 426
ApaI G_GGCC'C
Cuts at: 0 231 378 1167 1515
Size: 231 147 789 348
Fragments arranged by size:
789 348 231 147
ApoI r'AATT_y
Cuts at: 0 949 1515
Size: 949 566
AvaI C'yCGr_G
Cuts at: 0 162 519 1455 1515
Size: 162 357 936 60
Fragments arranged by size:
936 357 162 60
BanI G'GyrC_C
Cuts at: 0 574 1515
Size: 574 941
BanII G_rGCy'C
Cuts at: 0 231 378 1167 1515
Size: 231 147 789 348
Fragments arranged by size:
789 348 231 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 796 817 1515
Size: 796 21 698
Fragments arranged by size:
796 698 21
BciVI GTATCCnnnnn_n'
Cuts at: 0 326 508 1515
Size: 326 182 1007
Fragments arranged by size:
1007 326 182
BclI T'GATC_A
Cuts at: 0 749 1515
Size: 749 766
BglI GCCn_nnn'nGGC
Cuts at: 0 235 1515
Size: 235 1280
BglII A'GATC_T
Cuts at: 0 756 1496 1515
Size: 756 740 19
Fragments arranged by size:
756 740 19
Bme1580I G_kGCm'C
Cuts at: 0 231 257 378 551 998 1167 1515
Size: 231 26 121 173 447 169 348
Fragments arranged by size:
447 348 231 173 169 121 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 811 1515
Size: 552 259 704
Fragments arranged by size:
704 552 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 452 1273 1515
Size: 327 125 821 242
Fragments arranged by size:
821 327 242 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1515
Size: 1270 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 1381 1515
Size: 1381 134
BsaBI GATnn'nnATC
Cuts at: 0 889 1366 1515
Size: 889 477 149
Fragments arranged by size:
889 477 149
BsaWI w'CCGG_w
Cuts at: 0 1352 1478 1515
Size: 1352 126 37
Fragments arranged by size:
1352 126 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 325 355 573 603 1515
Size: 325 30 218 30 912
Fragments arranged by size:
912 325 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 212 1000 1270 1515
Size: 77 135 788 270 245
Fragments arranged by size:
788 270 245 135 77
BseYI C'CCAG_C
Cuts at: 0 94 287 1296 1515
Size: 94 193 1009 219
Fragments arranged by size:
1009 219 193 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1515
Size: 32 1483
BsiEI CG_ry'CG
Cuts at: 0 1100 1515
Size: 1100 415
BsiHKAI G_wGCw'C
Cuts at: 0 917 1153 1515
Size: 917 236 362
Fragments arranged by size:
917 362 236
Bsp1286I G_dGCh'C
Cuts at: 0 231 257 378 551 917 998 1153 1167
Size: 231 26 121 173 366 81 155 14
Cuts at: 1167 1515
Size: 348
Fragments arranged by size:
366 348 231 173 155 121 81 26
14
BsrDI GCAATG_nn'
Cuts at: 0 915 1515
Size: 915 600
BstAPI GCAn_nnn'nTGC
Cuts at: 0 498 1515
Size: 498 1017
BstBI TT'CG_AA
Cuts at: 0 978 1515
Size: 978 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 1515
Size: 114 1401
BstYI r'GATC_y
Cuts at: 0 710 756 890 1496 1515
Size: 710 46 134 606 19
Fragments arranged by size:
710 606 134 46 19
BtgI C'CryG_G
Cuts at: 0 1271 1515
Size: 1271 244
DraIII CAC_nnn'GTG
Cuts at: 0 55 1515
Size: 55 1460
EaeI y'GGCC_r
Cuts at: 0 125 173 1097 1268 1515
Size: 125 48 924 171 247
Fragments arranged by size:
924 247 171 125 48
EagI C'GGCC_G
Cuts at: 0 1097 1515
Size: 1097 418
EarI CTCTTCn'nnn_
Cuts at: 0 962 1216 1515
Size: 962 254 299
Fragments arranged by size:
962 299 254
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 350 1515
Size: 80 270 1165
Fragments arranged by size:
1165 270 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 452 1273 1451 1513 1515
Size: 327 125 821 178 62 2
Fragments arranged by size:
821 327 178 125 62 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 967 1255 1515
Size: 628 339 288 260
Fragments arranged by size:
628 339 288 260
EcoO109I rG'GnC_Cy
Cuts at: 0 227 228 374 375 425 621 1248 1515
Size: 227 1 146 1 50 196 627 267
Fragments arranged by size:
627 267 227 196 146 50 1 1
EcoRI G'AATT_C
Cuts at: 0 949 1515
Size: 949 566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 813 1515
Size: 813 702
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 563 595 741 748 773 780
Size: 145 32 386 32 146 7 25 7
Cuts at: 780 1393 1425 1515
Size: 613 32 90
Fragments arranged by size:
613 386 146 145 90 32 32 32
25 7 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1515
Size: 716 799
MscI TGG'CCA
Cuts at: 0 127 1515
Size: 127 1388
MslI CAynn'nnrTG
Cuts at: 0 1051 1372 1515
Size: 1051 321 143
Fragments arranged by size:
1051 321 143
MspA1I CmG'CkG
Cuts at: 0 46 544 702 1005 1515
Size: 46 498 158 303 510
Fragments arranged by size:
510 498 303 158 46
NspI r_CATG'y
Cuts at: 0 102 183 210 1062 1266 1515
Size: 102 81 27 852 204 249
Fragments arranged by size:
852 249 204 102 81 27
PasI CC'CwG_GG
Cuts at: 0 65 223 232 370 421 433 481 1515
Size: 65 158 9 138 51 12 48 1034
Fragments arranged by size:
1034 158 138 65 51 48 12 9
PciI A'CATG_T
Cuts at: 0 179 1058 1515
Size: 179 879 457
Fragments arranged by size:
879 457 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 58 481 1515
Size: 58 423 1034
Fragments arranged by size:
1034 423 58
PfoI T'CCnGG_A
Cuts at: 0 343 1153 1397 1515
Size: 343 810 244 118
Fragments arranged by size:
810 343 244 118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1515
Size: 1117 32 366
Fragments arranged by size:
1117 366 32
PpuMI rG'GwC_Cy
Cuts at: 0 621 1248 1515
Size: 621 627 267
Fragments arranged by size:
627 621 267
PshAI GACnn'nnGTC
Cuts at: 0 817 1515
Size: 817 698
PspOMI G'GGCC_C
Cuts at: 0 227 374 1163 1515
Size: 227 147 789 352
Fragments arranged by size:
789 352 227 147
PvuII CAG'CTG
Cuts at: 0 46 1515
Size: 46 1469
SapI GCTCTTCn'nnn_
Cuts at: 0 1216 1515
Size: 1216 299
SexAI A'CCwGG_T
Cuts at: 0 51 1169 1515
Size: 51 1118 346
Fragments arranged by size:
1118 346 51
SmaI CCC'GGG
Cuts at: 0 521 1515
Size: 521 994
SmlI C'TyrA_G
Cuts at: 0 1285 1515
Size: 1285 230
SphI G_CATG'C
Cuts at: 0 102 1266 1515
Size: 102 1164 249
Fragments arranged by size:
1164 249 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1405 1515
Size: 1405 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 573 605 1515
Size: 573 32 910
Fragments arranged by size:
910 573 32
XmaI C'CCGG_G
Cuts at: 0 519 1515
Size: 519 996
XmnI GAAnn'nnTTC
Cuts at: 0 643 1515
Size: 643 872
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BciVI BclI BglI BglII Bme1580I BmrI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrDI BstAPI BstBI BstXI
BstYI BtgI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I MmeI MscI MslI
MspA1I NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PvuII SapI SexAI SmaI SmlI SphI
TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BlpI BmgBI
BmtI BplI Bpu10I BsaAI BsaHI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
StyI SwaI TatI Tth111I XbaI XcmI XhoI ZraI