(Linear) (Six-base) MAPSORT of: KIBB5162.seq  Check: 6449  from: 1  to: 651



KIBB5162.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AfeI AGC'GCT

Cuts at:      0     270     651 
   Size:        270     381
 
AleI CACnn'nnGTG

Cuts at:      0     174     634     651 
   Size:        174     460      17

  Fragments arranged by size: 

                 460     174      17
 
BanI G'GyrC_C

Cuts at:      0     459     651 
   Size:        459     192
 
BclI T'GATC_A

Cuts at:      0      96     651 
   Size:         96     555
 
BglII A'GATC_T

Cuts at:      0     406     651 
   Size:        406     245
 
BlpI GC'TnA_GC

Cuts at:      0      49     220     651 
   Size:         49     171     431

  Fragments arranged by size: 

                 431     171      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     651 
   Size:        405     246
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     651 
   Size:         58     593
 
BsaBI GATnn'nnATC

Cuts at:      0     411     651 
   Size:        411     240
 
BsaWI w'CCGG_w

Cuts at:      0     641     651 
   Size:        641      10
 
BseYI C'CCAG_C

Cuts at:      0     522     651 
   Size:        522     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     651 
   Size:        334     317
 
BsiHKAI G_wGCw'C

Cuts at:      0     332     651 
   Size:        332     319
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     651 
   Size:        332     319
 
BsrFI r'CCGG_y

Cuts at:      0     604     651 
   Size:        604      47
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     176     514     651 
   Size:        176     338     137

  Fragments arranged by size: 

                 338     176     137
 
BstYI r'GATC_y

Cuts at:      0     406     651 
   Size:        406     245
 
BtgI C'CryG_G

Cuts at:      0     175     594     651 
   Size:        175     419      57

  Fragments arranged by size: 

                 419     175      57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     520     651 
   Size:        520     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     651 
   Size:        405     246
 
EcoO109I rG'GnC_Cy

Cuts at:      0     242     486     651 
   Size:        242     244     165

  Fragments arranged by size: 

                 244     242     165
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     235     651 
   Size:        235     416
 
HaeII r_GCGC'y

Cuts at:      0     272     651 
   Size:        272     379
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     530     562     651 
   Size:        530      32      89

  Fragments arranged by size: 

                 530      89      32
 
MslI CAynn'nnrTG

Cuts at:      0     160     174     634     651 
   Size:        160      14     460      17

  Fragments arranged by size: 

                 460     160      17      14
 
MspA1I CmG'CkG

Cuts at:      0     231     651 
   Size:        231     420
 
NspI r_CATG'y

Cuts at:      0     612     651 
   Size:        612      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     555     578     651 
   Size:        555      23      73

  Fragments arranged by size: 

                 555      73      23
 
PshAI GACnn'nnGTC

Cuts at:      0     478     651 
   Size:        478     173
 
PstI C_TGCA'G

Cuts at:      0      39     651 
   Size:         39     612
 
ScaI AGT'ACT

Cuts at:      0     352     651 
   Size:        352     299
 
SfcI C'TryA_G

Cuts at:      0      35     651 
   Size:         35     616
 
SmlI C'TyrA_G

Cuts at:      0      73     651 
   Size:         73     578
 
StyI C'CwwG_G

Cuts at:      0     203     651 
   Size:        203     448
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     351     651 
   Size:        351     300
 
TatI w'GTAC_w

Cuts at:      0     350     651 
   Size:        350     301
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     296     328     651 
   Size:        296      32     323

  Fragments arranged by size: 

                 323     296      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     352     651 
   Size:        352     299
 

 Enzymes that do cut:

     AfeI     AleI     BanI     BclI    BglII     BlpI     BpmI    BpuEI
    BsaBI    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI    BstXI
    BstYI     BtgI     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
     MslI   MspA1I     NspI    PflMI    PshAI     PstI     ScaI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI