Restriction map for
pF1KB5155
(Linear) (Six-base) MAPSORT of: KIBB5155.seq Check: 9943 from: 1 to: 1533
KIBB5155.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 0 1474 1533
Size: 1474 59
AleI CACnn'nnGTG
Cuts at: 0 745 1421 1533
Size: 745 676 112
Fragments arranged by size:
745 676 112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 1533
Size: 709 32 792
Fragments arranged by size:
792 709 32
ApoI r'AATT_y
Cuts at: 0 1225 1533
Size: 1225 308
BanI G'GyrC_C
Cuts at: 0 1396 1533
Size: 1396 137
BanII G_rGCy'C
Cuts at: 0 56 576 1533
Size: 56 520 957
Fragments arranged by size:
957 520 56
BbvCI CC'TCA_GC
Cuts at: 0 847 1533
Size: 847 686
BglI GCCn_nnn'nGGC
Cuts at: 0 169 1533
Size: 169 1364
BglII A'GATC_T
Cuts at: 0 433 1302 1533
Size: 433 869 231
Fragments arranged by size:
869 433 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 743 1533
Size: 21 722 790
Fragments arranged by size:
790 722 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1533
Size: 649 884
Bpu10I CC'TnA_GC
Cuts at: 0 847 1533
Size: 847 686
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 239 269 1188 1218 1533
Size: 239 30 919 30 315
Fragments arranged by size:
919 315 239 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 260 1421 1533
Size: 1 259 1161 112
Fragments arranged by size:
1161 259 112 1
BseYI C'CCAG_C
Cuts at: 0 48 89 1533
Size: 48 41 1444
Fragments arranged by size:
1444 48 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1533
Size: 793 740
BsiHKAI G_wGCw'C
Cuts at: 0 576 742 1533
Size: 576 166 791
Fragments arranged by size:
791 576 166
BsmI GAATG_Cn'
Cuts at: 0 493 1533
Size: 493 1040
Bsp1286I G_dGCh'C
Cuts at: 0 56 576 742 1533
Size: 56 520 166 791
Fragments arranged by size:
791 520 166 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 728 1533
Size: 728 805
BsrDI GCAATG_nn'
Cuts at: 0 701 1181 1533
Size: 701 480 352
Fragments arranged by size:
701 480 352
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1305 1533
Size: 627 678 228
Fragments arranged by size:
678 627 228
BstYI r'GATC_y
Cuts at: 0 287 433 983 1302 1533
Size: 287 146 550 319 231
Fragments arranged by size:
550 319 287 231 146
BtgI C'CryG_G
Cuts at: 0 1000 1533
Size: 1000 533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 284 519 1533
Size: 284 235 1014
Fragments arranged by size:
1014 284 235
BtsI GCAGTG_nn'
Cuts at: 0 463 518 908 1052 1073 1449 1533
Size: 463 55 390 144 21 376 84
Fragments arranged by size:
463 390 376 144 84 55 21
DraI TTT'AAA
Cuts at: 0 1224 1533
Size: 1224 309
EaeI y'GGCC_r
Cuts at: 0 438 698 894 1167 1533
Size: 438 260 196 273 366
Fragments arranged by size:
438 366 273 260 196
EarI CTCTTCn'nnn_
Cuts at: 0 877 1235 1533
Size: 877 358 298
Fragments arranged by size:
877 358 298
EcoICRI GAG'CTC
Cuts at: 0 574 1533
Size: 574 959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1533
Size: 649 884
EcoO109I rG'GnC_Cy
Cuts at: 0 412 1005 1533
Size: 412 593 528
Fragments arranged by size:
593 528 412
EcoRV GAT'ATC
Cuts at: 0 372 1533
Size: 372 1161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1493 1533
Size: 1493 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 401 433 1188 1220 1533
Size: 401 32 755 32 313
Fragments arranged by size:
755 401 313 32 32
HincII GTy'rAC
Cuts at: 0 1365 1533
Size: 1365 168
HindIII A'AGCT_T
Cuts at: 0 303 1533
Size: 303 1230
MfeI C'AATT_G
Cuts at: 0 1151 1533
Size: 1151 382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 459 502 601 779 1396 1533
Size: 180 279 43 99 178 617 137
Fragments arranged by size:
617 279 180 178 137 99 43
MscI TGG'CCA
Cuts at: 0 440 700 896 1169 1533
Size: 440 260 196 273 364
Fragments arranged by size:
440 364 273 260 196
MslI CAynn'nnrTG
Cuts at: 0 538 595 694 745 1421 1533
Size: 538 57 99 51 676 112
Fragments arranged by size:
676 538 112 99 57 51
MspA1I CmG'CkG
Cuts at: 0 892 1533
Size: 892 641
NcoI C'CATG_G
Cuts at: 0 1000 1533
Size: 1000 533
NspI r_CATG'y
Cuts at: 0 1056 1533
Size: 1056 477
PflMI CCAn_nnn'nTGG
Cuts at: 0 1378 1533
Size: 1378 155
PpuMI rG'GwC_Cy
Cuts at: 0 412 1005 1533
Size: 412 593 528
Fragments arranged by size:
593 528 412
PvuII CAG'CTG
Cuts at: 0 892 1533
Size: 892 641
SacI G_AGCT'C
Cuts at: 0 576 1533
Size: 576 957
SanDI GG'GwC_CC
Cuts at: 0 1005 1533
Size: 1005 528
ScaI AGT'ACT
Cuts at: 0 296 1533
Size: 296 1237
SfcI C'TryA_G
Cuts at: 0 1491 1533
Size: 1491 42
StuI AGG'CCT
Cuts at: 0 473 973 1533
Size: 473 500 560
Fragments arranged by size:
560 500 473
StyI C'CwwG_G
Cuts at: 0 620 1000 1515 1533
Size: 620 380 515 18
Fragments arranged by size:
620 515 380 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 1533
Size: 337 1196
TatI w'GTAC_w
Cuts at: 0 294 1460 1480 1533
Size: 294 1166 20 53
Fragments arranged by size:
1166 294 53 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 293 325 743 775 1162 1194 1533
Size: 293 32 418 32 387 32 339
Fragments arranged by size:
418 387 339 293 32 32 32
Enzymes that do cut:
AccI AleI AloI ApoI BanI BanII BbvCI BglI
BglII BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BstXI BstYI BtgI
BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NspI PflMI PpuMI PvuII SacI
SanDI ScaI SfcI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI