Restriction map for
pF1KB5147
(Linear) (Six-base) MAPSORT of: KIBB5147.seq Check: 7129 from: 1 to: 1062
KIBB5147.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 373 522 1062
Size: 373 149 540
Fragments arranged by size:
540 373 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1062
Size: 76 986
AflIII A'CryG_T
Cuts at: 0 1050 1062
Size: 1050 12
AlwNI CAG_nnn'CTG
Cuts at: 0 294 1062
Size: 294 768
BanI G'GyrC_C
Cuts at: 0 373 522 1062
Size: 373 149 540
Fragments arranged by size:
540 373 149
BanII G_rGCy'C
Cuts at: 0 93 1062
Size: 93 969
BbsI GAAGACnn'nnnn_
Cuts at: 0 931 1062
Size: 931 131
BbvCI CC'TCA_GC
Cuts at: 0 299 839 1062
Size: 299 540 223
Fragments arranged by size:
540 299 223
BciVI GTATCCnnnnn_n'
Cuts at: 0 688 1062
Size: 688 374
BfrBI ATG'CAT
Cuts at: 0 760 1062
Size: 760 302
BmgBI CAC'GTC
Cuts at: 0 600 1062
Size: 600 462
BmrI ACTGGGnnnn_n'
Cuts at: 0 355 1013 1062
Size: 355 658 49
Fragments arranged by size:
658 355 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 236 1062
Size: 236 826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 159 206 764 1062
Size: 16 143 47 558 298
Fragments arranged by size:
558 298 143 47 16
Bpu10I CC'TnA_GC
Cuts at: 0 299 839 1062
Size: 299 540 223
Fragments arranged by size:
540 299 223
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 1062
Size: 347 715
BsaAI yAC'GTr
Cuts at: 0 1051 1062
Size: 1051 11
BsaBI GATnn'nnATC
Cuts at: 0 665 1062
Size: 665 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 515 545 762 777 792 807 1062
Size: 515 30 217 15 15 15 255
Fragments arranged by size:
515 255 217 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 365 490 1062
Size: 19 346 125 572
Fragments arranged by size:
572 346 125 19
BsmI GAATG_Cn'
Cuts at: 0 45 1062
Size: 45 1017
BsmBI CGTCTCn'nnnn_
Cuts at: 0 31 590 1062
Size: 31 559 472
Fragments arranged by size:
559 472 31
Bsp1286I G_dGCh'C
Cuts at: 0 93 1062
Size: 93 969
BstYI r'GATC_y
Cuts at: 0 876 1062
Size: 876 186
Bsu36I CC'TnA_GG
Cuts at: 0 7 1062
Size: 7 1055
BtgI C'CryG_G
Cuts at: 0 74 477 1062
Size: 74 403 585
Fragments arranged by size:
585 403 74
BtsI GCAGTG_nn'
Cuts at: 0 396 1062
Size: 396 666
DraI TTT'AAA
Cuts at: 0 626 1062
Size: 626 436
DraIII CAC_nnn'GTG
Cuts at: 0 75 1062
Size: 75 987
EaeI y'GGCC_r
Cuts at: 0 384 902 1062
Size: 384 518 160
Fragments arranged by size:
518 384 160
EarI CTCTTCn'nnn_
Cuts at: 0 570 911 1062
Size: 570 341 151
Fragments arranged by size:
570 341 151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 76 159 206 764 1062
Size: 16 60 83 47 558 298
Fragments arranged by size:
558 298 83 60 47 16
EcoRV GAT'ATC
Cuts at: 0 639 1062
Size: 639 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 662 694 1062
Size: 246 32 384 32 368
Fragments arranged by size:
384 368 246 32 32
HindIII A'AGCT_T
Cuts at: 0 963 1062
Size: 963 99
KpnI G_GTAC'C
Cuts at: 0 377 526 1062
Size: 377 149 536
Fragments arranged by size:
536 377 149
MscI TGG'CCA
Cuts at: 0 386 904 1062
Size: 386 518 158
Fragments arranged by size:
518 386 158
MspA1I CmG'CkG
Cuts at: 0 139 335 1062
Size: 139 196 727
Fragments arranged by size:
727 196 139
NcoI C'CATG_G
Cuts at: 0 477 1062
Size: 477 585
NsiI A_TGCA'T
Cuts at: 0 762 1062
Size: 762 300
NspI r_CATG'y
Cuts at: 0 831 1062
Size: 831 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 294 363 1062
Size: 75 219 69 699
Fragments arranged by size:
699 219 75 69
PfoI T'CCnGG_A
Cuts at: 0 544 1062
Size: 544 518
PmlI CAC'GTG
Cuts at: 0 1051 1062
Size: 1051 11
PvuII CAG'CTG
Cuts at: 0 335 1062
Size: 335 727
SfcI C'TryA_G
Cuts at: 0 330 511 929 1062
Size: 330 181 418 133
Fragments arranged by size:
418 330 181 133
StyI C'CwwG_G
Cuts at: 0 477 1062
Size: 477 585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 777 809 1062
Size: 777 32 253
Fragments arranged by size:
777 253 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 291 542 1062
Size: 291 251 520
Fragments arranged by size:
520 291 251
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI BanI BanII BbsI BbvCI
BciVI BfrBI BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaXI BseRI BsmI BsmBI Bsp1286I BstYI
Bsu36I BtgI BtsI DraI DraIII EaeI EarI Eco57MI
EcoRV Hin4I HindIII KpnI MscI MspA1I NcoI NsiI
NspI PflMI PfoI PmlI PvuII SfcI StyI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BclI
BglI BglII BlpI Bme1580I BmtI BpuEI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII HincII HpaI KasI MfeI MluI MmeI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI