Restriction map for
pF1KB5140
(Linear) (Six-base) MAPSORT of: KIBB5140.seq Check: 2843 from: 1 to: 1179
KIBB5140.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 884 1179
Size: 884 295
AfeI AGC'GCT
Cuts at: 0 67 1179
Size: 67 1112
AleI CACnn'nnGTG
Cuts at: 0 154 916 993 1179
Size: 154 762 77 186
Fragments arranged by size:
762 186 154 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 1179
Size: 427 752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 1179
Size: 640 32 507
Fragments arranged by size:
640 507 32
AlwNI CAG_nnn'CTG
Cuts at: 0 645 1021 1179
Size: 645 376 158
Fragments arranged by size:
645 376 158
AvaI C'yCGr_G
Cuts at: 0 28 397 793 826 1004 1179
Size: 28 369 396 33 178 175
Fragments arranged by size:
396 369 178 175 33 28
BanI G'GyrC_C
Cuts at: 0 50 170 226 371 551 981 1179
Size: 50 120 56 145 180 430 198
Fragments arranged by size:
430 198 180 145 120 56 50
BanII G_rGCy'C
Cuts at: 0 439 874 1179
Size: 439 435 305
Fragments arranged by size:
439 435 305
BbeI G_GCGC'C
Cuts at: 0 54 174 230 555 1179
Size: 54 120 56 325 624
Fragments arranged by size:
624 325 120 56 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 507 1179
Size: 507 672
BbvCI CC'TCA_GC
Cuts at: 0 175 682 1179
Size: 175 507 497
Fragments arranged by size:
507 497 175
BglI GCCn_nnn'nGGC
Cuts at: 0 48 401 1179
Size: 48 353 778
Fragments arranged by size:
778 353 48
BglII A'GATC_T
Cuts at: 0 479 1179
Size: 479 700
Bme1580I G_kGCm'C
Cuts at: 0 374 397 792 986 1179
Size: 374 23 395 194 193
Fragments arranged by size:
395 374 194 193 23
BmgBI CAC'GTC
Cuts at: 0 914 1179
Size: 914 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 295 667 949 1179
Size: 286 9 372 282 230
Fragments arranged by size:
372 286 282 230 9
Bpu10I CC'TnA_GC
Cuts at: 0 175 682 1179
Size: 175 507 497
Fragments arranged by size:
507 497 175
BsaI GGTCTCn'nnnn_
Cuts at: 0 340 346 1179
Size: 340 6 833
Fragments arranged by size:
833 340 6
BsaAI yAC'GTr
Cuts at: 0 72 1179
Size: 72 1107
BsaBI GATnn'nnATC
Cuts at: 0 1040 1179
Size: 1040 139
BsaHI Gr'CG_yC
Cuts at: 0 51 171 227 292 552 755 778 1179
Size: 51 120 56 65 260 203 23 401
Fragments arranged by size:
401 260 203 120 65 56 51 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 640 670 1179
Size: 571 30 39 30 509
Fragments arranged by size:
571 509 39 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 510 579 1099 1102 1179
Size: 52 458 69 520 3 77
Fragments arranged by size:
520 458 77 69 52 3
BseYI C'CCAG_C
Cuts at: 0 1060 1120 1179
Size: 1060 60 59
Fragments arranged by size:
1060 60 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 652 1017 1179
Size: 118 534 365 162
Fragments arranged by size:
534 365 162 118
BsiEI CG_ry'CG
Cuts at: 0 60 255 711 1179
Size: 60 195 456 468
Fragments arranged by size:
468 456 195 60
BsiHKAI G_wGCw'C
Cuts at: 0 272 874 1005 1179
Size: 272 602 131 174
Fragments arranged by size:
602 272 174 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 698 729 1179
Size: 698 31 450
Fragments arranged by size:
698 450 31
Bsp1286I G_dGCh'C
Cuts at: 0 272 374 397 439 792 874 986 1005
Size: 272 102 23 42 353 82 112 19
Cuts at: 1005 1179
Size: 174
Fragments arranged by size:
353 272 174 112 102 82 42 23
19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 1179
Size: 170 1009
BsrBI CCG'CTC
Cuts at: 0 632 1179
Size: 632 547
BsrFI r'CCGG_y
Cuts at: 0 86 1064 1179
Size: 86 978 115
Fragments arranged by size:
978 115 86
BssHII G'CGCG_C
Cuts at: 0 498 1179
Size: 498 681
BssSI C'ACGA_G
Cuts at: 0 745 967 1179
Size: 745 222 212
Fragments arranged by size:
745 222 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1021 1179
Size: 1021 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 1068 1179
Size: 156 912 111
Fragments arranged by size:
912 156 111
BstYI r'GATC_y
Cuts at: 0 479 1179
Size: 479 700
BtgI C'CryG_G
Cuts at: 0 46 54 255 440 530 845 1179
Size: 46 8 201 185 90 315 334
Fragments arranged by size:
334 315 201 185 90 46 8
BtsI GCAGTG_nn'
Cuts at: 0 172 1005 1179
Size: 172 833 174
Fragments arranged by size:
833 174 172
DraIII CAC_nnn'GTG
Cuts at: 0 287 531 600 1179
Size: 287 244 69 579
Fragments arranged by size:
579 287 244 69
EaeI y'GGCC_r
Cuts at: 0 57 84 252 323 1179
Size: 57 27 168 71 856
Fragments arranged by size:
856 168 71 57 27
EagI C'GGCC_G
Cuts at: 0 57 252 1179
Size: 57 195 927
Fragments arranged by size:
927 195 57
EarI CTCTTCn'nnn_
Cuts at: 0 223 340 721 1179
Size: 223 117 381 458
Fragments arranged by size:
458 381 223 117
EcoICRI GAG'CTC
Cuts at: 0 872 1179
Size: 872 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 295 667 949 1179
Size: 286 9 372 282 230
Fragments arranged by size:
372 286 282 230 9
HaeII r_GCGC'y
Cuts at: 0 54 69 174 230 555 1179
Size: 54 15 105 56 325 624
Fragments arranged by size:
624 325 105 56 54 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 1153 1179
Size: 571 32 550 26
Fragments arranged by size:
571 550 32 26
HincII GTy'rAC
Cuts at: 0 303 1179
Size: 303 876
KasI G'GCGC_C
Cuts at: 0 50 170 226 551 1179
Size: 50 120 56 325 628
Fragments arranged by size:
628 325 120 56 50
MslI CAynn'nnrTG
Cuts at: 0 154 850 916 993 1179
Size: 154 696 66 77 186
Fragments arranged by size:
696 186 154 77 66
MspA1I CmG'CkG
Cuts at: 0 48 56 257 666 732 1179
Size: 48 8 201 409 66 447
Fragments arranged by size:
447 409 201 66 48 8
NaeI GCC'GGC
Cuts at: 0 1066 1179
Size: 1066 113
NarI GG'CG_CC
Cuts at: 0 51 171 227 552 1179
Size: 51 120 56 325 627
Fragments arranged by size:
627 325 120 56 51
NcoI C'CATG_G
Cuts at: 0 440 1179
Size: 440 739
NgoMIV G'CCGG_C
Cuts at: 0 1064 1179
Size: 1064 115
NotI GC'GGCC_GC
Cuts at: 0 57 1179
Size: 57 1122
NspI r_CATG'y
Cuts at: 0 471 860 1179
Size: 471 389 319
Fragments arranged by size:
471 389 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 236 645 1179
Size: 236 409 534
Fragments arranged by size:
534 409 236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 1179
Size: 640 32 507
Fragments arranged by size:
640 507 32
PspXI vC'TCGA_Gb
Cuts at: 0 826 1179
Size: 826 353
PstI C_TGCA'G
Cuts at: 0 167 425 1179
Size: 167 258 754
Fragments arranged by size:
754 258 167
SacI G_AGCT'C
Cuts at: 0 874 1179
Size: 874 305
SacII CC_GC'GG
Cuts at: 0 49 57 258 1179
Size: 49 8 201 921
Fragments arranged by size:
921 201 49 8
SapI GCTCTTCn'nnn_
Cuts at: 0 223 1179
Size: 223 956
SexAI A'CCwGG_T
Cuts at: 0 878 1179
Size: 878 301
SfcI C'TryA_G
Cuts at: 0 163 421 1179
Size: 163 258 758
Fragments arranged by size:
758 258 163
SfoI GGC'GCC
Cuts at: 0 52 172 228 553 1179
Size: 52 120 56 325 626
Fragments arranged by size:
626 325 120 56 52
SmaI CCC'GGG
Cuts at: 0 30 1179
Size: 30 1149
SmlI C'TyrA_G
Cuts at: 0 793 826 1004 1179
Size: 793 33 178 175
Fragments arranged by size:
793 178 175 33
SrfI GCCC'GGGC
Cuts at: 0 30 1179
Size: 30 1149
StuI AGG'CCT
Cuts at: 0 607 1179
Size: 607 572
StyI C'CwwG_G
Cuts at: 0 440 1172 1179
Size: 440 732 7
Fragments arranged by size:
732 440 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 691 1179
Size: 364 327 488
Fragments arranged by size:
488 364 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 151 183 271 303 1179
Size: 73 32 46 32 88 32 876
Fragments arranged by size:
876 88 73 46 32 32 32
XhoI C'TCGA_G
Cuts at: 0 793 826 1004 1179
Size: 793 33 178 175
Fragments arranged by size:
793 178 175 33
XmaI C'CCGG_G
Cuts at: 0 28 1179
Size: 28 1151
XmnI GAAnn'nnTTC
Cuts at: 0 451 1179
Size: 451 728
Enzymes that do cut:
AccI AfeI AleI AlfI AloI AlwNI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
DraIII EaeI EagI EarI EcoICRI Eco57MI HaeII Hin4I
HincII KasI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PflMI PpiI PspXI PstI SacI SacII
SapI SexAI SfcI SfoI SmaI SmlI SrfI StuI
StyI TaqII TstI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmrI
BmtI BplI BpuEI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NheI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PsrI PvuI PvuII RsrII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI TatI Tth111I XbaI XcmI ZraI