Restriction map for
pF1KB5134
(Linear) (Six-base) MAPSORT of: KIBB5134.seq Check: 5003 from: 1 to: 2388
KIBB5134.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 98 126 2388
Size: 98 28 2262
Fragments arranged by size:
2262 98 28
AccI GT'mk_AC
Cuts at: 0 815 2388
Size: 815 1573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1569 2388
Size: 533 1036 819
Fragments arranged by size:
1036 819 533
AfeI AGC'GCT
Cuts at: 0 148 2388
Size: 148 2240
AflIII A'CryG_T
Cuts at: 0 371 2388
Size: 371 2017
AlwNI CAG_nnn'CTG
Cuts at: 0 1192 2388
Size: 1192 1196
ApaLI G'TGCA_C
Cuts at: 0 1054 2388
Size: 1054 1334
ApoI r'AATT_y
Cuts at: 0 430 1617 2388
Size: 430 1187 771
Fragments arranged by size:
1187 771 430
AseI AT'TA_AT
Cuts at: 0 455 1298 2388
Size: 455 843 1090
Fragments arranged by size:
1090 843 455
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2080 2113 2388
Size: 2080 33 275
Fragments arranged by size:
2080 275 33
BanI G'GyrC_C
Cuts at: 0 86 110 1195 1852 2388
Size: 86 24 1085 657 536
Fragments arranged by size:
1085 657 536 86 24
BanII G_rGCy'C
Cuts at: 0 366 1139 2123 2248 2269 2388
Size: 366 773 984 125 21 119
Fragments arranged by size:
984 773 366 125 119 21
BbeI G_GCGC'C
Cuts at: 0 1856 2388
Size: 1856 532
BbsI GAAGACnn'nnnn_
Cuts at: 0 2018 2388
Size: 2018 370
BciVI GTATCCnnnnn_n'
Cuts at: 0 610 2388
Size: 610 1778
BclI T'GATC_A
Cuts at: 0 1497 1718 2388
Size: 1497 221 670
Fragments arranged by size:
1497 670 221
Bme1580I G_kGCm'C
Cuts at: 0 89 113 1058 1800 2388
Size: 89 24 945 742 588
Fragments arranged by size:
945 742 588 89 24
BmgBI CAC'GTC
Cuts at: 0 1788 2388
Size: 1788 600
BmrI ACTGGGnnnn_n'
Cuts at: 0 360 1411 2331 2388
Size: 360 1051 920 57
Fragments arranged by size:
1051 920 360 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 2026 2388
Size: 436 1590 362
Fragments arranged by size:
1590 436 362
Bpu10I CC'TnA_GC
Cuts at: 0 383 1843 2263 2388
Size: 383 1460 420 125
Fragments arranged by size:
1460 420 383 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 2388
Size: 478 1910
BsaAI yAC'GTr
Cuts at: 0 699 2388
Size: 699 1689
BsaBI GATnn'nnATC
Cuts at: 0 1864 2388
Size: 1864 524
BsaHI Gr'CG_yC
Cuts at: 0 1853 2388
Size: 1853 535
BsaWI w'CCGG_w
Cuts at: 0 468 2388
Size: 468 1920
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 2105 2135 2187 2217 2388
Size: 409 30 1666 30 52 30 171
Fragments arranged by size:
1666 409 171 52 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 703 2192 2388
Size: 205 498 1489 196
Fragments arranged by size:
1489 498 205 196
BseYI C'CCAG_C
Cuts at: 0 161 2388
Size: 161 2227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1562 2388
Size: 463 1099 826
Fragments arranged by size:
1099 826 463
BsiEI CG_ry'CG
Cuts at: 0 1266 2388
Size: 1266 1122
BsiHKAI G_wGCw'C
Cuts at: 0 1058 1849 2269 2388
Size: 1058 791 420 119
Fragments arranged by size:
1058 791 420 119
Bsp1286I G_dGCh'C
Cuts at: 0 89 113 366 1058 1139 1800 1849 2123
Size: 89 24 253 692 81 661 49 274
Cuts at: 2123 2248 2269 2388
Size: 125 21 119
Fragments arranged by size:
692 661 274 253 125 119 89 81
49 24 21
BspEI T'CCGG_A
Cuts at: 0 468 2388
Size: 468 1920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 126 2388
Size: 98 28 2262
Fragments arranged by size:
2262 98 28
BsrBI CCG'CTC
Cuts at: 0 78 2388
Size: 78 2310
BsrDI GCAATG_nn'
Cuts at: 0 1086 2388
Size: 1086 1302
BsrFI r'CCGG_y
Cuts at: 0 1837 2388
Size: 1837 551
BsrGI T'GTAC_A
Cuts at: 0 593 2388
Size: 593 1795
BssSI C'ACGA_G
Cuts at: 0 481 2166 2388
Size: 481 1685 222
Fragments arranged by size:
1685 481 222
BstAPI GCAn_nnn'nTGC
Cuts at: 0 50 2388
Size: 50 2338
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 720 2142 2388
Size: 345 375 1422 246
Fragments arranged by size:
1422 375 345 246
BstYI r'GATC_y
Cuts at: 0 879 2388
Size: 879 1509
BstZ17I GTA'TAC
Cuts at: 0 816 2388
Size: 816 1572
BtgI C'CryG_G
Cuts at: 0 105 1199 2195 2388
Size: 105 1094 996 193
Fragments arranged by size:
1094 996 193 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1739 2388
Size: 1739 649
BtsI GCAGTG_nn'
Cuts at: 0 1798 2388
Size: 1798 590
ClaI AT'CG_AT
Cuts at: 0 1262 2388
Size: 1262 1126
DraIII CAC_nnn'GTG
Cuts at: 0 702 2388
Size: 702 1686
EaeI y'GGCC_r
Cuts at: 0 409 1421 1562 2125 2252 2279 2388
Size: 409 1012 141 563 127 27 109
Fragments arranged by size:
1012 563 409 141 127 109 27
EarI CTCTTCn'nnn_
Cuts at: 0 350 858 2388
Size: 350 508 1530
Fragments arranged by size:
1530 508 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 752 2388
Size: 752 1636
EcoICRI GAG'CTC
Cuts at: 0 2267 2388
Size: 2267 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 533 1569 2026 2388
Size: 436 97 1036 457 362
Fragments arranged by size:
1036 457 436 362 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1475 2388
Size: 1475 913
EcoO109I rG'GnC_Cy
Cuts at: 0 1431 2258 2326 2388
Size: 1431 827 68 62
Fragments arranged by size:
1431 827 68 62
EcoRI G'AATT_C
Cuts at: 0 430 2388
Size: 430 1958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1713 2388
Size: 1713 675
HaeII r_GCGC'y
Cuts at: 0 150 584 1856 2134 2388
Size: 150 434 1272 278 254
Fragments arranged by size:
1272 434 278 254 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 301 333 365 409 441 2105
Size: 229 32 40 32 32 44 32 1664
Cuts at: 2105 2137 2388
Size: 32 251
Fragments arranged by size:
1664 251 229 44 40 32 32 32
32 32
HincII GTy'rAC
Cuts at: 0 1443 2388
Size: 1443 945
KasI G'GCGC_C
Cuts at: 0 1852 2388
Size: 1852 536
MfeI C'AATT_G
Cuts at: 0 1526 2388
Size: 1526 862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 407 1541 2388
Size: 326 81 1134 847
Fragments arranged by size:
1134 847 326 81
MscI TGG'CCA
Cuts at: 0 1423 2388
Size: 1423 965
MslI CAynn'nnrTG
Cuts at: 0 59 301 376 2388
Size: 59 242 75 2012
Fragments arranged by size:
2012 242 75 59
MspA1I CmG'CkG
Cuts at: 0 1189 2388
Size: 1189 1199
NaeI GCC'GGC
Cuts at: 0 1839 2388
Size: 1839 549
NarI GG'CG_CC
Cuts at: 0 1853 2388
Size: 1853 535
NcoI C'CATG_G
Cuts at: 0 105 2388
Size: 105 2283
NdeI CA'TA_TG
Cuts at: 0 731 2388
Size: 731 1657
NgoMIV G'CCGG_C
Cuts at: 0 1837 2388
Size: 1837 551
NspI r_CATG'y
Cuts at: 0 48 1219 2109 2388
Size: 48 1171 890 279
Fragments arranged by size:
1171 890 279 48
PasI CC'CwG_GG
Cuts at: 0 847 2388
Size: 847 1541
PflMI CCAn_nnn'nTGG
Cuts at: 0 889 1309 2388
Size: 889 420 1079
Fragments arranged by size:
1079 889 420
PfoI T'CCnGG_A
Cuts at: 0 446 2388
Size: 446 1942
PmlI CAC'GTG
Cuts at: 0 699 2388
Size: 699 1689
PpuMI rG'GwC_Cy
Cuts at: 0 1431 2258 2326 2388
Size: 1431 827 68 62
Fragments arranged by size:
1431 827 68 62
PshAI GACnn'nnGTC
Cuts at: 0 778 2388
Size: 778 1610
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 250 282 2388
Size: 250 32 2106
Fragments arranged by size:
2106 250 32
PvuI CG_AT'CG
Cuts at: 0 1266 2388
Size: 1266 1122
PvuII CAG'CTG
Cuts at: 0 1189 2388
Size: 1189 1199
SacI G_AGCT'C
Cuts at: 0 2269 2388
Size: 2269 119
SanDI GG'GwC_CC
Cuts at: 0 2258 2388
Size: 2258 130
SfcI C'TryA_G
Cuts at: 0 142 1001 1026 2314 2388
Size: 142 859 25 1288 74
Fragments arranged by size:
1288 859 142 74 25
SfoI GGC'GCC
Cuts at: 0 1854 2388
Size: 1854 534
SphI G_CATG'C
Cuts at: 0 48 1219 2388
Size: 48 1171 1169
Fragments arranged by size:
1171 1169 48
SspI AAT'ATT
Cuts at: 0 918 2388
Size: 918 1470
StuI AGG'CCT
Cuts at: 0 1822 2388
Size: 1822 566
StyI C'CwwG_G
Cuts at: 0 105 683 713 2388
Size: 105 578 30 1675
Fragments arranged by size:
1675 578 105 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 790 2388
Size: 712 78 1598
Fragments arranged by size:
1598 712 78
TatI w'GTAC_w
Cuts at: 0 194 368 593 2102 2388
Size: 194 174 225 1509 286
Fragments arranged by size:
1509 286 225 194 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1284 1316 2187 2219 2388
Size: 1284 32 871 32 169
Fragments arranged by size:
1284 871 169 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 411 1416 2050 2388
Size: 342 69 1005 634 338
Fragments arranged by size:
1005 634 342 338 69
XmnI GAAnn'nnTTC
Cuts at: 0 175 1604 2388
Size: 175 1429 784
Fragments arranged by size:
1429 784 175
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaLI ApoI
AseI BaeI BanI BanII BbeI BbsI BciVI BclI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PshAI PsrI
PvuI PvuII SacI SanDI SfcI SfoI SphI SspI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvaI AvrII BamHI BbvCI
BcgI BfrBI BglI BglII BlpI BmtI BplI BpuEI
BsiWI BsmI BsmBI BspHI BssHII BstBI BstEII Bsu36I
CspCI DraI DrdI EagI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MluI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PsiI PspOMI PspXI PstI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI
Tth111I XbaI XhoI XmaI ZraI