Restriction map for
pF1KB5128
(Linear) (Six-base) MAPSORT of: KIBB5128.seq Check: 2450 from: 1 to: 2385
KIBB5128.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1496 2385
Size: 1496 889
AatII G_ACGT'C
Cuts at: 0 1328 2385
Size: 1328 1057
AccI GT'mk_AC
Cuts at: 0 2060 2385
Size: 2060 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2317 2385
Size: 2317 68
AfeI AGC'GCT
Cuts at: 0 1239 2385
Size: 1239 1146
AlwNI CAG_nnn'CTG
Cuts at: 0 750 2385
Size: 750 1635
ApaI G_GGCC'C
Cuts at: 0 2188 2385
Size: 2188 197
ApaLI G'TGCA_C
Cuts at: 0 724 2385
Size: 724 1661
ApoI r'AATT_y
Cuts at: 0 17 1103 1191 2385
Size: 17 1086 88 1194
Fragments arranged by size:
1194 1086 88 17
AvaI C'yCGr_G
Cuts at: 0 79 188 1434 1727 1782 2179 2385
Size: 79 109 1246 293 55 397 206
Fragments arranged by size:
1246 397 293 206 109 79 55
AvrII C'CTAG_G
Cuts at: 0 658 2385
Size: 658 1727
BanI G'GyrC_C
Cuts at: 0 1438 1591 1722 2175 2218 2385
Size: 1438 153 131 453 43 167
Fragments arranged by size:
1438 453 167 153 131 43
BanII G_rGCy'C
Cuts at: 0 281 2188 2385
Size: 281 1907 197
Fragments arranged by size:
1907 281 197
BbeI G_GCGC'C
Cuts at: 0 1595 2385
Size: 1595 790
BbsI GAAGACnn'nnnn_
Cuts at: 0 487 2385
Size: 487 1898
BbvCI CC'TCA_GC
Cuts at: 0 467 2385
Size: 467 1918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 245 2385
Size: 211 34 2140
Fragments arranged by size:
2140 211 34
BclI T'GATC_A
Cuts at: 0 378 689 2385
Size: 378 311 1696
Fragments arranged by size:
1696 378 311
BglI GCCn_nnn'nGGC
Cuts at: 0 139 1570 2385
Size: 139 1431 815
Fragments arranged by size:
1431 815 139
BlpI GC'TnA_GC
Cuts at: 0 1885 2385
Size: 1885 500
Bme1580I G_kGCm'C
Cuts at: 0 728 1441 1727 2180 2188 2385
Size: 728 713 286 453 8 197
Fragments arranged by size:
728 713 453 286 197 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 2385
Size: 138 2247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 2385
Size: 260 2125
Bpu10I CC'TnA_GC
Cuts at: 0 467 2385
Size: 467 1918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1801 2385
Size: 1801 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 1209 1225 1323 1512 2385
Size: 1209 16 98 189 873
Fragments arranged by size:
1209 873 189 98 16
BsaAI yAC'GTr
Cuts at: 0 1974 2385
Size: 1974 411
BsaHI Gr'CG_yC
Cuts at: 0 1325 1592 2385
Size: 1325 267 793
Fragments arranged by size:
1325 793 267
BsaWI w'CCGG_w
Cuts at: 0 1120 2385
Size: 1120 1265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 983 2157 2385
Size: 619 364 1174 228
Fragments arranged by size:
1174 619 364 228
BseYI C'CCAG_C
Cuts at: 0 1295 1997 2385
Size: 1295 702 388
Fragments arranged by size:
1295 702 388
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 763 1333 1384 1695 1954 2157 2385
Size: 601 162 570 51 311 259 203 228
Fragments arranged by size:
601 570 311 259 228 203 162 51
BsiEI CG_ry'CG
Cuts at: 0 1736 2385
Size: 1736 649
BsiHKAI G_wGCw'C
Cuts at: 0 281 413 728 1979 2114 2385
Size: 281 132 315 1251 135 271
Fragments arranged by size:
1251 315 281 271 135 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1075 2385
Size: 1075 1310
Bsp1286I G_dGCh'C
Cuts at: 0 281 413 728 1441 1727 1979 2114 2180
Size: 281 132 315 713 286 252 135 66
Cuts at: 2180 2188 2385
Size: 8 197
Fragments arranged by size:
713 315 286 281 252 197 135 132
66 8
BspEI T'CCGG_A
Cuts at: 0 1120 2385
Size: 1120 1265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 735 1305 1496 2015 2385
Size: 735 570 191 519 370
Fragments arranged by size:
735 570 519 370 191
BsrBI CCG'CTC
Cuts at: 0 119 2385
Size: 119 2266
BsrDI GCAATG_nn'
Cuts at: 0 495 2385
Size: 495 1890
BsrFI r'CCGG_y
Cuts at: 0 333 2385
Size: 333 2052
BssSI C'ACGA_G
Cuts at: 0 631 1915 2385
Size: 631 1284 470
Fragments arranged by size:
1284 631 470
BstAPI GCAn_nnn'nTGC
Cuts at: 0 219 2385
Size: 219 2166
BstBI TT'CG_AA
Cuts at: 0 681 923 2385
Size: 681 242 1462
Fragments arranged by size:
1462 681 242
BstEII G'GTnAC_C
Cuts at: 0 650 1452 1752 2385
Size: 650 802 300 633
Fragments arranged by size:
802 650 633 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 1764 2385
Size: 150 1614 621
Fragments arranged by size:
1614 621 150
BstYI r'GATC_y
Cuts at: 0 147 2385
Size: 147 2238
Bsu36I CC'TnA_GG
Cuts at: 0 1543 2018 2385
Size: 1543 475 367
Fragments arranged by size:
1543 475 367
BtgI C'CryG_G
Cuts at: 0 1602 1823 2385
Size: 1602 221 562
Fragments arranged by size:
1602 562 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1242 2385
Size: 1242 1143
BtsI GCAGTG_nn'
Cuts at: 0 1416 2385
Size: 1416 969
EaeI y'GGCC_r
Cuts at: 0 140 1527 1733 1955 2385
Size: 140 1387 206 222 430
Fragments arranged by size:
1387 430 222 206 140
EagI C'GGCC_G
Cuts at: 0 1733 2385
Size: 1733 652
EarI CTCTTCn'nnn_
Cuts at: 0 269 2101 2107 2385
Size: 269 1832 6 278
Fragments arranged by size:
1832 278 269 6
EcoICRI GAG'CTC
Cuts at: 0 279 2385
Size: 279 2106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 2317 2385
Size: 260 2057 68
Fragments arranged by size:
2057 260 68
EcoO109I rG'GnC_Cy
Cuts at: 0 747 1479 1563 2184 2185 2225 2385
Size: 747 732 84 621 1 40 160
Fragments arranged by size:
747 732 621 160 84 40 1
EcoRI G'AATT_C
Cuts at: 0 17 1103 2385
Size: 17 1086 1282
Fragments arranged by size:
1282 1086 17
FspI TGC'GCA
Cuts at: 0 1870 2385
Size: 1870 515
FspAI rTGC'GCAy
Cuts at: 0 1870 2385
Size: 1870 515
HaeII r_GCGC'y
Cuts at: 0 640 1241 1421 1595 1633 2385
Size: 640 601 180 174 38 752
Fragments arranged by size:
752 640 601 180 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 634 666 1417 1449 1765 1797
Size: 6 32 596 32 751 32 316 32
Cuts at: 1797 1978 2010 2385
Size: 181 32 375
Fragments arranged by size:
751 596 375 316 181 32 32 32
32 32 6
KasI G'GCGC_C
Cuts at: 0 1591 2385
Size: 1591 794
MfeI C'AATT_G
Cuts at: 0 776 2385
Size: 776 1609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1346 2385
Size: 83 1263 1039
Fragments arranged by size:
1263 1039 83
MscI TGG'CCA
Cuts at: 0 142 2385
Size: 142 2243
MslI CAynn'nnrTG
Cuts at: 0 673 1003 1309 2385
Size: 673 330 306 1076
Fragments arranged by size:
1076 673 330 306
MspA1I CmG'CkG
Cuts at: 0 225 1604 1689 1812 2385
Size: 225 1379 85 123 573
Fragments arranged by size:
1379 573 225 123 85
NarI GG'CG_CC
Cuts at: 0 1592 2385
Size: 1592 793
NdeI CA'TA_TG
Cuts at: 0 735 2385
Size: 735 1650
PfoI T'CCnGG_A
Cuts at: 0 323 365 749 2385
Size: 323 42 384 1636
Fragments arranged by size:
1636 384 323 42
PmlI CAC'GTG
Cuts at: 0 1974 2385
Size: 1974 411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2275 2307 2385
Size: 2275 32 78
Fragments arranged by size:
2275 78 32
PpuMI rG'GwC_Cy
Cuts at: 0 747 1479 2225 2385
Size: 747 732 746 160
Fragments arranged by size:
747 746 732 160
PshAI GACnn'nnGTC
Cuts at: 0 1087 2385
Size: 1087 1298
PspOMI G'GGCC_C
Cuts at: 0 2184 2385
Size: 2184 201
PspXI vC'TCGA_Gb
Cuts at: 0 79 2385
Size: 79 2306
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 287 319 2385
Size: 287 32 2066
Fragments arranged by size:
2066 287 32
PstI C_TGCA'G
Cuts at: 0 1571 1694 2385
Size: 1571 123 691
Fragments arranged by size:
1571 691 123
PvuII CAG'CTG
Cuts at: 0 225 1812 2385
Size: 225 1587 573
Fragments arranged by size:
1587 573 225
SacI G_AGCT'C
Cuts at: 0 281 2385
Size: 281 2104
SacII CC_GC'GG
Cuts at: 0 1605 2385
Size: 1605 780
SanDI GG'GwC_CC
Cuts at: 0 2225 2385
Size: 2225 160
SapI GCTCTTCn'nnn_
Cuts at: 0 269 2101 2385
Size: 269 1832 284
Fragments arranged by size:
1832 284 269
SbfI CC_TGCA'GG
Cuts at: 0 1571 2385
Size: 1571 814
SexAI A'CCwGG_T
Cuts at: 0 1748 2063 2385
Size: 1748 315 322
Fragments arranged by size:
1748 322 315
SfcI C'TryA_G
Cuts at: 0 992 1567 1690 2385
Size: 992 575 123 695
Fragments arranged by size:
992 695 575 123
SfoI GGC'GCC
Cuts at: 0 1593 2385
Size: 1593 792
SmaI CCC'GGG
Cuts at: 0 190 1729 2385
Size: 190 1539 656
Fragments arranged by size:
1539 656 190
SmlI C'TyrA_G
Cuts at: 0 79 1816 2385
Size: 79 1737 569
Fragments arranged by size:
1737 569 79
SspI AAT'ATT
Cuts at: 0 392 2306 2385
Size: 392 1914 79
Fragments arranged by size:
1914 392 79
StuI AGG'CCT
Cuts at: 0 974 2385
Size: 974 1411
StyI C'CwwG_G
Cuts at: 0 143 658 1757 2385
Size: 143 515 1099 628
Fragments arranged by size:
1099 628 515 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1241 1296 1366 2385
Size: 1241 55 70 1019
Fragments arranged by size:
1241 1019 70 55
TatI w'GTAC_w
Cuts at: 0 303 1244 2385
Size: 303 941 1141
Fragments arranged by size:
1141 941 303
Tth111I GACn'n_nGTC
Cuts at: 0 2229 2385
Size: 2229 156
XhoI C'TCGA_G
Cuts at: 0 79 2385
Size: 79 2306
XmaI C'CCGG_G
Cuts at: 0 188 1727 2385
Size: 188 1539 658
Fragments arranged by size:
1539 658 188
ZraI GAC'GTC
Cuts at: 0 1326 2385
Size: 1326 1059
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AlwNI ApaI ApaLI
ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I KasI MfeI
MmeI MscI MslI MspA1I NarI NdeI PfoI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SacII SanDI SapI SbfI SexAI SfcI SfoI
SmaI SmlI SspI StuI StyI TaqII TatI Tth111I
XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI BaeI BamHI BciVI BfrBI
BglII BmgBI BmrI BmtI BsaBI BsaXI BsiWI BsmI
BspHI BsrGI BssHII BstZ17I ClaI CspCI DraI DraIII
DrdI EciI EcoNI EcoRV FalI FseI HincII HindIII
HpaI KpnI MluI NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PsiI PvuI RsrII SalI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI TstI XbaI XcmI
XmnI