Restriction map for
pF1KB5120
(Linear) (Six-base) MAPSORT of: KIBB5120.seq Check: 7567 from: 1 to: 1260
KIBB5120.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1077 1260
Size: 1077 183
AccI GT'mk_AC
Cuts at: 0 416 967 1260
Size: 416 551 293
Fragments arranged by size:
551 416 293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1260
Size: 44 1216
AleI CACnn'nnGTG
Cuts at: 0 178 1260
Size: 178 1082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 1260
Size: 64 1196
ApaLI G'TGCA_C
Cuts at: 0 1004 1260
Size: 1004 256
ApoI r'AATT_y
Cuts at: 0 868 874 1260
Size: 868 6 386
Fragments arranged by size:
868 386 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 566 599 1260
Size: 566 33 661
Fragments arranged by size:
661 566 33
BamHI G'GATC_C
Cuts at: 0 567 1260
Size: 567 693
BanI G'GyrC_C
Cuts at: 0 669 1260
Size: 669 591
BanII G_rGCy'C
Cuts at: 0 293 1260
Size: 293 967
BbsI GAAGACnn'nnnn_
Cuts at: 0 903 1260
Size: 903 357
BlpI GC'TnA_GC
Cuts at: 0 743 1260
Size: 743 517
Bme1580I G_kGCm'C
Cuts at: 0 672 1008 1260
Size: 672 336 252
Fragments arranged by size:
672 336 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 336 982 1226 1260
Size: 277 59 646 244 34
Fragments arranged by size:
646 277 244 59 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1260
Size: 1076 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 1202 1260
Size: 1202 58
BsaWI w'CCGG_w
Cuts at: 0 405 930 1260
Size: 405 525 330
Fragments arranged by size:
525 405 330
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 227 257 440 470 1260
Size: 227 30 183 30 790
Fragments arranged by size:
790 227 183 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 641 1127 1260
Size: 641 486 133
Fragments arranged by size:
641 486 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1055 1260
Size: 70 985 205
Fragments arranged by size:
985 205 70
BsiHKAI G_wGCw'C
Cuts at: 0 293 1008 1260
Size: 293 715 252
Fragments arranged by size:
715 293 252
Bsp1286I G_dGCh'C
Cuts at: 0 293 672 1008 1260
Size: 293 379 336 252
Fragments arranged by size:
379 336 293 252
BspEI T'CCGG_A
Cuts at: 0 405 1260
Size: 405 855
BspHI T'CATG_A
Cuts at: 0 299 1260
Size: 299 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1077 1260
Size: 1077 183
BsrFI r'CCGG_y
Cuts at: 0 45 1260
Size: 45 1215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 523 1260
Size: 523 737
BstYI r'GATC_y
Cuts at: 0 567 1260
Size: 567 693
BstZ17I GTA'TAC
Cuts at: 0 417 968 1260
Size: 417 551 292
Fragments arranged by size:
551 417 292
BtgI C'CryG_G
Cuts at: 0 1212 1260
Size: 1212 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 9 44 101 136 1260
Size: 9 35 57 35 1124
Fragments arranged by size:
1124 57 35 35 9
DraI TTT'AAA
Cuts at: 0 873 1260
Size: 873 387
DrdI GACnn_nn'nnGTC
Cuts at: 0 437 1039 1260
Size: 437 602 221
Fragments arranged by size:
602 437 221
EarI CTCTTCn'nnn_
Cuts at: 0 1081 1260
Size: 1081 179
EcoICRI GAG'CTC
Cuts at: 0 291 1260
Size: 291 969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 277 336 982 1226 1260
Size: 44 233 59 646 244 34
Fragments arranged by size:
646 244 233 59 44 34
EcoO109I rG'GnC_Cy
Cuts at: 0 813 1032 1260
Size: 813 219 228
Fragments arranged by size:
813 228 219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 238 1260
Size: 238 1022
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 688 706 720 738 1260
Size: 227 32 429 18 14 18 522
Fragments arranged by size:
522 429 227 32 18 18 14
HindIII A'AGCT_T
Cuts at: 0 998 1260
Size: 998 262
MfeI C'AATT_G
Cuts at: 0 938 1260
Size: 938 322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 850 939 1260
Size: 545 305 89 321
Fragments arranged by size:
545 321 305 89
MslI CAynn'nnrTG
Cuts at: 0 178 1260
Size: 178 1082
MspA1I CmG'CkG
Cuts at: 0 492 618 1159 1260
Size: 492 126 541 101
Fragments arranged by size:
541 492 126 101
PpuMI rG'GwC_Cy
Cuts at: 0 813 1032 1260
Size: 813 219 228
Fragments arranged by size:
813 228 219
PshAI GACnn'nnGTC
Cuts at: 0 907 1260
Size: 907 353
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 478 510 844 876 1260
Size: 478 32 334 32 384
Fragments arranged by size:
478 384 334 32 32
PvuII CAG'CTG
Cuts at: 0 492 618 1159 1260
Size: 492 126 541 101
Fragments arranged by size:
541 492 126 101
SacI G_AGCT'C
Cuts at: 0 293 1260
Size: 293 967
SanDI GG'GwC_CC
Cuts at: 0 1032 1260
Size: 1032 228
SfcI C'TryA_G
Cuts at: 0 435 663 1260
Size: 435 228 597
Fragments arranged by size:
597 435 228
SmlI C'TyrA_G
Cuts at: 0 1091 1260
Size: 1091 169
SspI AAT'ATT
Cuts at: 0 767 1260
Size: 767 493
StyI C'CwwG_G
Cuts at: 0 898 1260
Size: 898 362
SwaI ATTT'AAAT
Cuts at: 0 873 1260
Size: 873 387
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApaLI ApoI BaeI
BamHI BanI BanII BbsI BlpI Bme1580I BpmI BpuEI
BsaI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrFI BstXI BstYI BstZ17I BtgI CspCI
DraI DrdI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I
HindIII MfeI MmeI MslI MspA1I PpuMI PshAI PsrI
PvuII SacI SanDI SfcI SmlI SspI StyI SwaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BseYI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI
ClaI DraIII EaeI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI