Restriction map for
pF1KB5107
(Linear) (Six-base) MAPSORT of: KIBB5107.seq Check: 8191 from: 1 to: 2205
KIBB5107.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 539 2205
Size: 539 1666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 307 844 881 1119 1352 1627 1777
Size: 79 228 537 37 238 233 275 150
Cuts at: 1777 2161 2205
Size: 384 44
Fragments arranged by size:
537 384 275 238 233 228 150 79
44 37
AfeI AGC'GCT
Cuts at: 0 1107 1300 2205
Size: 1107 193 905
Fragments arranged by size:
1107 905 193
AflIII A'CryG_T
Cuts at: 0 1449 1451 1968 2205
Size: 1449 2 517 237
Fragments arranged by size:
1449 517 237 2
AhdI GACnn_n'nnGTC
Cuts at: 0 735 1953 2205
Size: 735 1218 252
Fragments arranged by size:
1218 735 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 287 2205
Size: 287 1918
AlwNI CAG_nnn'CTG
Cuts at: 0 852 2007 2205
Size: 852 1155 198
Fragments arranged by size:
1155 852 198
ApaI G_GGCC'C
Cuts at: 0 885 1116 2205
Size: 885 231 1089
Fragments arranged by size:
1089 885 231
ApaLI G'TGCA_C
Cuts at: 0 1594 2205
Size: 1594 611
AvaI C'yCGr_G
Cuts at: 0 1648 2205
Size: 1648 557
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1438 1471 2205
Size: 1438 33 734
Fragments arranged by size:
1438 734 33
BamHI G'GATC_C
Cuts at: 0 211 1362 1974 2205
Size: 211 1151 612 231
Fragments arranged by size:
1151 612 231 211
BanI G'GyrC_C
Cuts at: 0 263 1191 1743 2205
Size: 263 928 552 462
Fragments arranged by size:
928 552 462 263
BanII G_rGCy'C
Cuts at: 0 26 191 775 885 1116 1282 1709 2205
Size: 26 165 584 110 231 166 427 496
Fragments arranged by size:
584 496 427 231 166 165 110 26
BbeI G_GCGC'C
Cuts at: 0 1747 2205
Size: 1747 458
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 948 1169 2205
Size: 219 729 221 1036
Fragments arranged by size:
1036 729 221 219
BglI GCCn_nnn'nGGC
Cuts at: 0 511 1189 2205
Size: 511 678 1016
Fragments arranged by size:
1016 678 511
BglII A'GATC_T
Cuts at: 0 128 2205
Size: 128 2077
BlpI GC'TnA_GC
Cuts at: 0 1028 1997 2205
Size: 1028 969 208
Fragments arranged by size:
1028 969 208
Bme1580I G_kGCm'C
Cuts at: 0 266 885 1116 1185 1598 2205
Size: 266 619 231 69 413 607
Fragments arranged by size:
619 607 413 266 231 69
BmgBI CAC'GTC
Cuts at: 0 147 1067 2205
Size: 147 920 1138
Fragments arranged by size:
1138 920 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 840 1847 2205
Size: 840 1007 358
Fragments arranged by size:
1007 840 358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1812 2205
Size: 1812 393
Bpu10I CC'TnA_GC
Cuts at: 0 622 2205
Size: 622 1583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 106 178 547 2205
Size: 1 105 72 369 1658
Fragments arranged by size:
1658 369 105 72 1
BsaI GGTCTCn'nnnn_
Cuts at: 0 551 799 2205
Size: 551 248 1406
Fragments arranged by size:
1406 551 248
BsaAI yAC'GTr
Cuts at: 0 310 1254 1452 1971 2102 2205
Size: 310 944 198 519 131 103
Fragments arranged by size:
944 519 310 198 131 103
BsaBI GATnn'nnATC
Cuts at: 0 133 356 2205
Size: 133 223 1849
Fragments arranged by size:
1849 223 133
BsaHI Gr'CG_yC
Cuts at: 0 116 1744 2205
Size: 116 1628 461
Fragments arranged by size:
1628 461 116
BsaWI w'CCGG_w
Cuts at: 0 320 797 2205
Size: 320 477 1408
Fragments arranged by size:
1408 477 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1202 1232 2205
Size: 1202 30 973
Fragments arranged by size:
1202 973 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 496 604 1699 2205
Size: 181 315 108 1095 506
Fragments arranged by size:
1095 506 315 181 108
BseYI C'CCAG_C
Cuts at: 0 11 1102 1108 1612 2135 2168 2205
Size: 11 1091 6 504 523 33 37
Fragments arranged by size:
1091 523 504 37 33 11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 517 1183 1531 1561 2205
Size: 364 153 666 348 30 644
Fragments arranged by size:
666 644 364 348 153 30
BsiHKAI G_wGCw'C
Cuts at: 0 191 859 1598 2205
Size: 191 668 739 607
Fragments arranged by size:
739 668 607 191
BsmI GAATG_Cn'
Cuts at: 0 1802 2205
Size: 1802 403
Bsp1286I G_dGCh'C
Cuts at: 0 26 191 266 775 859 885 1116 1185
Size: 26 165 75 509 84 26 231 69
Cuts at: 1185 1282 1598 1709 2205
Size: 97 316 111 496
Fragments arranged by size:
509 496 316 231 165 111 97 84
75 69 26 26
BspEI T'CCGG_A
Cuts at: 0 320 2205
Size: 320 1885
BspHI T'CATG_A
Cuts at: 0 1712 2205
Size: 1712 493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 1992 2205
Size: 539 1453 213
Fragments arranged by size:
1453 539 213
BsrBI CCG'CTC
Cuts at: 0 6 33 1527 2205
Size: 6 27 1494 678
Fragments arranged by size:
1494 678 27 6
BsrFI r'CCGG_y
Cuts at: 0 908 1150 2205
Size: 908 242 1055
Fragments arranged by size:
1055 908 242
BsrGI T'GTAC_A
Cuts at: 0 1811 2205
Size: 1811 394
BssSI C'ACGA_G
Cuts at: 0 643 1178 2205
Size: 643 535 1027
Fragments arranged by size:
1027 643 535
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2007 2205
Size: 2007 198
BstEII G'GTnAC_C
Cuts at: 0 2022 2205
Size: 2022 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1323 1740 1950 2205
Size: 1323 417 210 255
Fragments arranged by size:
1323 417 255 210
BstYI r'GATC_y
Cuts at: 0 128 211 1362 1499 1974 2205
Size: 128 83 1151 137 475 231
Fragments arranged by size:
1151 475 231 137 128 83
Bsu36I CC'TnA_GG
Cuts at: 0 75 692 1748 2205
Size: 75 617 1056 457
Fragments arranged by size:
1056 617 457 75
BtgI C'CryG_G
Cuts at: 0 1221 2017 2090 2205
Size: 1221 796 73 115
Fragments arranged by size:
1221 796 115 73
BtsI GCAGTG_nn'
Cuts at: 0 1028 1896 2205
Size: 1028 868 309
Fragments arranged by size:
1028 868 309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1256 1291 2205
Size: 1256 35 914
Fragments arranged by size:
1256 914 35
DraIII CAC_nnn'GTG
Cuts at: 0 1206 2205
Size: 1206 999
EaeI y'GGCC_r
Cuts at: 0 28 503 1145 1398 2205
Size: 28 475 642 253 807
Fragments arranged by size:
807 642 475 253 28
EarI CTCTTCn'nnn_
Cuts at: 0 451 1367 2205
Size: 451 916 838
Fragments arranged by size:
916 838 451
EcoICRI GAG'CTC
Cuts at: 0 189 2205
Size: 189 2016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 307 844 881 1119 1352 1627 1777
Size: 79 228 537 37 238 233 275 150
Cuts at: 1777 1812 2161 2205
Size: 35 349 44
Fragments arranged by size:
537 349 275 238 233 228 150 79
44 37 35
EcoO109I rG'GnC_Cy
Cuts at: 0 441 882 1112 1602 1947 2067 2205
Size: 441 441 230 490 345 120 138
Fragments arranged by size:
490 441 441 345 230 138 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 1026 2056 2205
Size: 109 917 1030 149
Fragments arranged by size:
1030 917 149 109
FspI TGC'GCA
Cuts at: 0 1660 2205
Size: 1660 545
HaeII r_GCGC'y
Cuts at: 0 1109 1302 1747 1764 1989 2205
Size: 1109 193 445 17 225 216
Fragments arranged by size:
1109 445 225 216 193 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 721 753 1051 1083 1939 1971 2205
Size: 721 32 298 32 856 32 234
Fragments arranged by size:
856 721 298 234 32 32 32
HincII GTy'rAC
Cuts at: 0 702 2205
Size: 702 1503
HindIII A'AGCT_T
Cuts at: 0 2038 2205
Size: 2038 167
KasI G'GCGC_C
Cuts at: 0 1743 2205
Size: 1743 462
MfeI C'AATT_G
Cuts at: 0 1268 2205
Size: 1268 937
MscI TGG'CCA
Cuts at: 0 1147 1400 2205
Size: 1147 253 805
Fragments arranged by size:
1147 805 253
MslI CAynn'nnrTG
Cuts at: 0 269 1321 2205
Size: 269 1052 884
Fragments arranged by size:
1052 884 269
MspA1I CmG'CkG
Cuts at: 0 51 1122 1689 2055 2139 2172 2205
Size: 51 1071 567 366 84 33 33
Fragments arranged by size:
1071 567 366 84 51 33 33
NarI GG'CG_CC
Cuts at: 0 1744 2205
Size: 1744 461
NcoI C'CATG_G
Cuts at: 0 1221 2017 2090 2205
Size: 1221 796 73 115
Fragments arranged by size:
1221 796 115 73
NspI r_CATG'y
Cuts at: 0 843 955 2205
Size: 843 112 1250
Fragments arranged by size:
1250 843 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 974 2205
Size: 974 1231
PfoI T'CCnGG_A
Cuts at: 0 1639 2205
Size: 1639 566
PmlI CAC'GTG
Cuts at: 0 1452 1971 2205
Size: 1452 519 234
Fragments arranged by size:
1452 519 234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 2205
Size: 457 32 1716
Fragments arranged by size:
1716 457 32
PpuMI rG'GwC_Cy
Cuts at: 0 441 1602 1947 2067 2205
Size: 441 1161 345 120 138
Fragments arranged by size:
1161 441 345 138 120
PspOMI G'GGCC_C
Cuts at: 0 881 1112 2205
Size: 881 231 1093
Fragments arranged by size:
1093 881 231
PstI C_TGCA'G
Cuts at: 0 2012 2205
Size: 2012 193
PvuII CAG'CTG
Cuts at: 0 1122 1689 2055 2139 2205
Size: 1122 567 366 84 66
Fragments arranged by size:
1122 567 366 84 66
SacI G_AGCT'C
Cuts at: 0 191 2205
Size: 191 2014
SexAI A'CCwGG_T
Cuts at: 0 1232 1457 1949 2205
Size: 1232 225 492 256
Fragments arranged by size:
1232 492 256 225
SfcI C'TryA_G
Cuts at: 0 2008 2071 2205
Size: 2008 63 134
Fragments arranged by size:
2008 134 63
SfoI GGC'GCC
Cuts at: 0 1745 2205
Size: 1745 460
SmlI C'TyrA_G
Cuts at: 0 16 121 193 562 2205
Size: 16 105 72 369 1643
Fragments arranged by size:
1643 369 105 72 16
SphI G_CATG'C
Cuts at: 0 843 2205
Size: 843 1362
StuI AGG'CCT
Cuts at: 0 655 2205
Size: 655 1550
StyI C'CwwG_G
Cuts at: 0 104 1079 1221 1943 2017 2090 2205
Size: 104 975 142 722 74 73 115
Fragments arranged by size:
975 722 142 115 104 74 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 377 412 1092 2041 2205
Size: 377 35 680 949 164
Fragments arranged by size:
949 680 377 164 35
TatI w'GTAC_w
Cuts at: 0 680 1811 2205
Size: 680 1131 394
Fragments arranged by size:
1131 680 394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1875 1907 2205
Size: 1875 32 298
Fragments arranged by size:
1875 298 32
Tth111I GACn'n_nGTC
Cuts at: 0 316 2205
Size: 316 1889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 151 214 1155 2205
Size: 151 63 941 1050
Fragments arranged by size:
1050 941 151 63
XmnI GAAnn'nnTTC
Cuts at: 0 1516 2205
Size: 1516 689
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI
ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
KasI MfeI MscI MslI MspA1I NarI NcoI NspI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SexAI SfcI SfoI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BbvCI BcgI BciVI
BclI BfrBI BmtI BplI BsiEI BsiWI BsmBI BsrDI
BssHII BstBI BstZ17I BtgZI ClaI DraI DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspAI HpaI KpnI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmaI ZraI