Restriction map for
pF1KB5103
(Linear) (Six-base) MAPSORT of: KIBB5103.seq Check: 127 from: 1 to: 2100
KIBB5103.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1487 2100
Size: 1487 613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 788 1799 2100
Size: 25 763 1011 301
Fragments arranged by size:
1011 763 301 25
AflIII A'CryG_T
Cuts at: 0 1199 1526 2100
Size: 1199 327 574
Fragments arranged by size:
1199 574 327
AlwNI CAG_nnn'CTG
Cuts at: 0 1809 1832 2100
Size: 1809 23 268
Fragments arranged by size:
1809 268 23
ApaLI G'TGCA_C
Cuts at: 0 335 2100
Size: 335 1765
ApoI r'AATT_y
Cuts at: 0 439 776 1391 1444 1548 2100
Size: 439 337 615 53 104 552
Fragments arranged by size:
615 552 439 337 104 53
AvaI C'yCGr_G
Cuts at: 0 252 2100
Size: 252 1848
BanI G'GyrC_C
Cuts at: 0 113 263 691 2100
Size: 113 150 428 1409
Fragments arranged by size:
1409 428 150 113
BanII G_rGCy'C
Cuts at: 0 1286 2100
Size: 1286 814
BbeI G_GCGC'C
Cuts at: 0 267 2100
Size: 267 1833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1289 2100
Size: 1255 34 811
Fragments arranged by size:
1255 811 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 987 2100
Size: 987 1113
BclI T'GATC_A
Cuts at: 0 2046 2100
Size: 2046 54
BglI GCCn_nnn'nGGC
Cuts at: 0 1085 2100
Size: 1085 1015
BlpI GC'TnA_GC
Cuts at: 0 853 2100
Size: 853 1247
Bme1580I G_kGCm'C
Cuts at: 0 339 696 2100
Size: 339 357 1404
Fragments arranged by size:
1404 357 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 289 340 1546 1809 2100
Size: 196 93 51 1206 263 291
Fragments arranged by size:
1206 291 263 196 93 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 392 2100
Size: 392 1708
BsaAI yAC'GTr
Cuts at: 0 1527 2100
Size: 1527 573
BsaBI GATnn'nnATC
Cuts at: 0 1316 2100
Size: 1316 784
BsaHI Gr'CG_yC
Cuts at: 0 264 2100
Size: 264 1836
BsaWI w'CCGG_w
Cuts at: 0 1642 2100
Size: 1642 458
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 848 2100
Size: 120 728 1252
Fragments arranged by size:
1252 728 120
BseYI C'CCAG_C
Cuts at: 0 170 1883 2100
Size: 170 1713 217
Fragments arranged by size:
1713 217 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1784 1833 2100
Size: 1784 49 267
Fragments arranged by size:
1784 267 49
BsiHKAI G_wGCw'C
Cuts at: 0 339 1825 2100
Size: 339 1486 275
Fragments arranged by size:
1486 339 275
BsmI GAATG_Cn'
Cuts at: 0 862 1783 2100
Size: 862 921 317
Fragments arranged by size:
921 862 317
BsmBI CGTCTCn'nnnn_
Cuts at: 0 727 2100
Size: 727 1373
Bsp1286I G_dGCh'C
Cuts at: 0 339 696 1286 1825 2100
Size: 339 357 590 539 275
Fragments arranged by size:
590 539 357 339 275
BspHI T'CATG_A
Cuts at: 0 942 2100
Size: 942 1158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1806 2026 2100
Size: 1806 220 74
Fragments arranged by size:
1806 220 74
BsrBI CCG'CTC
Cuts at: 0 95 2100
Size: 95 2005
BsrGI T'GTAC_A
Cuts at: 0 1094 2100
Size: 1094 1006
BstYI r'GATC_y
Cuts at: 0 1749 2079 2100
Size: 1749 330 21
Fragments arranged by size:
1749 330 21
BstZ17I GTA'TAC
Cuts at: 0 1488 2100
Size: 1488 612
Bsu36I CC'TnA_GG
Cuts at: 0 1772 2100
Size: 1772 328
BtgI C'CryG_G
Cuts at: 0 38 1787 1856 2100
Size: 38 1749 69 244
Fragments arranged by size:
1749 244 69 38
BtsI GCAGTG_nn'
Cuts at: 0 1591 2100
Size: 1591 509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 907 942 2100
Size: 907 35 1158
Fragments arranged by size:
1158 907 35
EaeI y'GGCC_r
Cuts at: 0 35 2100
Size: 35 2065
EarI CTCTTCn'nnn_
Cuts at: 0 611 730 2100
Size: 611 119 1370
Fragments arranged by size:
1370 611 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 196 289 340 788 1546 1799 1809
Size: 25 171 93 51 448 758 253 10
Cuts at: 1809 2100
Size: 291
Fragments arranged by size:
758 448 291 253 171 93 51 25
10
EcoO109I rG'GnC_Cy
Cuts at: 0 1622 2100
Size: 1622 478
EcoRI G'AATT_C
Cuts at: 0 439 1391 1444 2100
Size: 439 952 53 656
Fragments arranged by size:
952 656 439 53
EcoRV GAT'ATC
Cuts at: 0 1981 2100
Size: 1981 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1999 2100
Size: 1999 101
HaeII r_GCGC'y
Cuts at: 0 141 267 2100
Size: 141 126 1833
Fragments arranged by size:
1833 141 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 1704 1736 2050 2082 2100
Size: 1055 32 617 32 314 32 18
Fragments arranged by size:
1055 617 314 32 32 32 18
KasI G'GCGC_C
Cuts at: 0 263 2100
Size: 263 1837
MslI CAynn'nnrTG
Cuts at: 0 1198 1937 2059 2100
Size: 1198 739 122 41
Fragments arranged by size:
1198 739 122 41
MspA1I CmG'CkG
Cuts at: 0 174 2100
Size: 174 1926
NarI GG'CG_CC
Cuts at: 0 264 2100
Size: 264 1836
NspI r_CATG'y
Cuts at: 0 1203 1928 2100
Size: 1203 725 172
Fragments arranged by size:
1203 725 172
PciI A'CATG_T
Cuts at: 0 1199 2100
Size: 1199 901
PpuMI rG'GwC_Cy
Cuts at: 0 1622 2100
Size: 1622 478
PstI C_TGCA'G
Cuts at: 0 1876 1903 2100
Size: 1876 27 197
Fragments arranged by size:
1876 197 27
PvuII CAG'CTG
Cuts at: 0 174 2100
Size: 174 1926
ScaI AGT'ACT
Cuts at: 0 407 2100
Size: 407 1693
SfcI C'TryA_G
Cuts at: 0 1396 1626 1872 1899 2100
Size: 1396 230 246 27 201
Fragments arranged by size:
1396 246 230 201 27
SfoI GGC'GCC
Cuts at: 0 265 2100
Size: 265 1835
SmaI CCC'GGG
Cuts at: 0 254 2100
Size: 254 1846
SpeI A'CTAG_T
Cuts at: 0 1615 2100
Size: 1615 485
StyI C'CwwG_G
Cuts at: 0 1225 2100
Size: 1225 875
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1968 2100
Size: 1968 132
TatI w'GTAC_w
Cuts at: 0 405 1094 2100
Size: 405 689 1006
Fragments arranged by size:
1006 689 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1511 1543 2100
Size: 1511 32 557
Fragments arranged by size:
1511 557 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1723 2100
Size: 1723 377
XmaI C'CCGG_G
Cuts at: 0 252 2100
Size: 252 1848
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BcgI BciVI BclI BglI BlpI Bme1580I
BpmI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrGI BstYI BstZ17I Bsu36I BtgI BtsI CspCI EaeI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
KasI MslI MspA1I NarI NspI PciI PpuMI PstI
PvuII ScaI SfcI SfoI SmaI SpeI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BfrBI BglII BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaXI BsiEI BsiWI BspEI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI