Restriction map for
pF1KB5099
(Linear) (Six-base) MAPSORT of: KIBB5099.seq Check: 33 from: 1 to: 2310
KIBB5099.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1424 1673 1681 2281 2310
Size: 640 784 249 8 600 29
Fragments arranged by size:
784 640 600 249 29 8
AflII C'TTAA_G
Cuts at: 0 1069 2191 2310
Size: 1069 1122 119
Fragments arranged by size:
1122 1069 119
AleI CACnn'nnGTG
Cuts at: 0 428 2310
Size: 428 1882
ApaI G_GGCC'C
Cuts at: 0 2202 2310
Size: 2202 108
ApoI r'AATT_y
Cuts at: 0 175 414 640 660 1005 1219 1541 2310
Size: 175 239 226 20 345 214 322 769
Fragments arranged by size:
769 345 322 239 226 214 175 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 591 624 2310
Size: 591 33 1686
Fragments arranged by size:
1686 591 33
BanII G_rGCy'C
Cuts at: 0 2202 2310
Size: 2202 108
BciVI GTATCCnnnnn_n'
Cuts at: 0 1378 2310
Size: 1378 932
BfrBI ATG'CAT
Cuts at: 0 184 1779 2310
Size: 184 1595 531
Fragments arranged by size:
1595 531 184
Bme1580I G_kGCm'C
Cuts at: 0 2202 2310
Size: 2202 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 2310
Size: 140 2170
BmtI G_CTAG'C
Cuts at: 0 37 2310
Size: 37 2273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 2310
Size: 422 1888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1486 2310
Size: 320 1166 824
Fragments arranged by size:
1166 824 320
BsaBI GATnn'nnATC
Cuts at: 0 1853 2310
Size: 1853 457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1598 2310
Size: 1598 712
BseYI C'CCAG_C
Cuts at: 0 1720 2153 2310
Size: 1720 433 157
Fragments arranged by size:
1720 433 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 387 2310
Size: 30 357 1923
Fragments arranged by size:
1923 357 30
BsiHKAI G_wGCw'C
Cuts at: 0 263 2131 2310
Size: 263 1868 179
Fragments arranged by size:
1868 263 179
BsmI GAATG_Cn'
Cuts at: 0 324 2232 2310
Size: 324 1908 78
Fragments arranged by size:
1908 324 78
Bsp1286I G_dGCh'C
Cuts at: 0 263 2131 2202 2310
Size: 263 1868 71 108
Fragments arranged by size:
1868 263 108 71
BspHI T'CATG_A
Cuts at: 0 551 629 1845 2310
Size: 551 78 1216 465
Fragments arranged by size:
1216 551 465 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 875 915 1698 2053 2148 2310
Size: 875 40 783 355 95 162
Fragments arranged by size:
875 783 355 162 95 40
BsrDI GCAATG_nn'
Cuts at: 0 1539 1837 1897 2234 2310
Size: 1539 298 60 337 76
Fragments arranged by size:
1539 337 298 76 60
BsrFI r'CCGG_y
Cuts at: 0 569 2310
Size: 569 1741
BstYI r'GATC_y
Cuts at: 0 141 1854 2310
Size: 141 1713 456
Fragments arranged by size:
1713 456 141
Bsu36I CC'TnA_GG
Cuts at: 0 2038 2310
Size: 2038 272
BtsI GCAGTG_nn'
Cuts at: 0 263 695 779 2310
Size: 263 432 84 1531
Fragments arranged by size:
1531 432 263 84
DraI TTT'AAA
Cuts at: 0 1323 1547 2310
Size: 1323 224 763
Fragments arranged by size:
1323 763 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1177 2310
Size: 1177 1133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 640 1424 1673 1681 2281 2310
Size: 422 218 784 249 8 600 29
Fragments arranged by size:
784 600 422 249 218 29 8
EcoO109I rG'GnC_Cy
Cuts at: 0 2042 2076 2198 2199 2310
Size: 2042 34 122 1 111
Fragments arranged by size:
2042 122 111 34 1
EcoRI G'AATT_C
Cuts at: 0 414 2310
Size: 414 1896
EcoRV GAT'ATC
Cuts at: 0 627 2310
Size: 627 1683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1017 2310
Size: 1017 1293
FspI TGC'GCA
Cuts at: 0 2049 2310
Size: 2049 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 959 991 2310
Size: 959 32 1319
Fragments arranged by size:
1319 959 32
HindIII A'AGCT_T
Cuts at: 0 392 743 2310
Size: 392 351 1567
Fragments arranged by size:
1567 392 351
MfeI C'AATT_G
Cuts at: 0 1565 2310
Size: 1565 745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1769 1882 2310
Size: 1769 113 428
Fragments arranged by size:
1769 428 113
MslI CAynn'nnrTG
Cuts at: 0 428 767 2310
Size: 428 339 1543
Fragments arranged by size:
1543 428 339
MspA1I CmG'CkG
Cuts at: 0 1813 2310
Size: 1813 497
NheI G'CTAG_C
Cuts at: 0 33 2310
Size: 33 2277
NsiI A_TGCA'T
Cuts at: 0 186 1781 2310
Size: 186 1595 529
Fragments arranged by size:
1595 529 186
PfoI T'CCnGG_A
Cuts at: 0 438 2310
Size: 438 1872
PpuMI rG'GwC_Cy
Cuts at: 0 2042 2076 2310
Size: 2042 34 234
Fragments arranged by size:
2042 234 34
PsiI TTA'TAA
Cuts at: 0 1331 2310
Size: 1331 979
PspOMI G'GGCC_C
Cuts at: 0 2198 2310
Size: 2198 112
PstI C_TGCA'G
Cuts at: 0 702 2310
Size: 702 1608
PvuII CAG'CTG
Cuts at: 0 1813 2310
Size: 1813 497
SfcI C'TryA_G
Cuts at: 0 698 2310
Size: 698 1612
SmlI C'TyrA_G
Cuts at: 0 335 1069 1465 2191 2310
Size: 335 734 396 726 119
Fragments arranged by size:
734 726 396 335 119
SspI AAT'ATT
Cuts at: 0 1207 1318 2310
Size: 1207 111 992
Fragments arranged by size:
1207 992 111
StyI C'CwwG_G
Cuts at: 0 720 2310
Size: 720 1590
TatI w'GTAC_w
Cuts at: 0 331 1282 1938 1980 2310
Size: 331 951 656 42 330
Fragments arranged by size:
951 656 331 330 42
Tth111I GACn'n_nGTC
Cuts at: 0 889 2310
Size: 889 1421
XmnI GAAnn'nnTTC
Cuts at: 0 395 1133 1455 2310
Size: 395 738 322 855
Fragments arranged by size:
855 738 395 322
Enzymes that do cut:
AcuI AflII AleI ApaI ApoI BaeI BanII BciVI
BfrBI Bme1580I BmrI BmtI BpmI BpuEI BsaBI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BstYI Bsu36I BtsI DraI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI Hin4I HindIII MfeI MmeI
MslI MspA1I NheI NsiI PfoI PpuMI PsiI PspOMI
PstI PvuII SfcI SmlI SspI StyI TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI BmgBI BplI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI EcoNI FseI FspAI HaeII HincII
HpaI KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TstI XbaI XcmI
XhoI XmaI ZraI